A new integrated genetic linkage map of the soybean

Abstract

A total of 391 simple sequence repeat

Mapping soybean aphid resistance genes in PI 567598B

“…The DNA was extracted with the CTAB (hexadecyltrimethylammonium bromide) method as described by Kisha et al (1997), and the concentration was determined with a ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). Simple sequence repeat (SSR) markers (Song et al 2004) were used to amplify the genomic DNA according to the PCR protocol described by Cregan and Quigley (1997), using a MJ Tetrad TM thermal cycler (MJ Research, Waltham, MA). PCR products were detected on 6 % non-denaturing polyacrylamide gels using a DASG-400-50 electrophoresis system (C.B.S.…”

“…Based on the 2010 phenotypic data, 10 resistant lines with the lowest DI values and 10 susceptible lines with the highest DI values were selected to form a resistant pool and a susceptible pool, respectively. Parental polymorphic SSR markers at approximately every 15 cM of the integrated soybean map of Song et al (2004) were selected to test the polymorphism between the two bulked DNA pools. The polymorphic markers between the two pools were chosen to genotype the individual lines in the two pools together with the two parents.…”

BARCSoySNP23: a panel of 23 selected SNPs for soybean cultivar identification

“…The sequence data from each amplicon were analyzed with PolyBayes SNP discovery software as described by Zhu et al (2003). SNPs were mapped in the Minsoy · Noir 1 recombinant inbred line soybean mapping population (Song et al 2004). The single base extension (SBE) method was used for the detection of SNPs (see below).…”

“…A total of 58 SNPs on the linkage map was selected ( Table 2). The SNPs were spaced at intervals of approximately 40 cM across the 20 consensus soybean linkage groups (Song et al 2004). …”

“…This is one of the five mapping populations recently used by Song et al (2004) to derive a consensus soybean genetic linkage map comprised of SSR, RFLP and other markers. A preliminary positioning of the SNPs relative to the known soybean map was deemed useful to ensure that the final selection of SNPs would not be closely linked and would generally span as much of the soybean genome as possible.…”