A New Interpretation of Serum Albumin Surface Passivation

Sweryda-Krawiec, Beata; Devaraj, Halagowder; Jacob, George; Hickman, James J.

doi:10.1021/la034870g

Cited by 122 publications

(115 citation statements)

References 24 publications

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“…Such findings were consistent with the fouling mechanisms of protein or lipopolysaccharide against a hydrophobic or hydrophilic surface. [61,62] More importantly, amphiphilic crosslinked…”

Section: Anti-fouling and Fouling-release Performance Of Hbfp (I) /Pementioning

confidence: 99%

Hyperbranched Fluoropolymers and their Hybridization into Complex Amphiphilic Crosslinked Copolymer Networks

Bartels¹,

Cheng²,

Powell³

et al. 2007

Macro Chemistry & Physics

100

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This feature article highlights three types of hyperbranched fluoropolymers (HBFPs) with different structural features, which were synthesized by either polycondensation of fluorinated ABx monomers or self‐condensing vinyl (co)polymerization of fluorinated inimers and/or fluorinated comonomers. Amphiphilic crosslinked networks with hybridization of these hydrophobic HBFPs and linear hydrophilic poly(ethylene glycol)s are also discussed. As microphase‐segregated materials with nanoscale surface heterogeneities, these networks possessed unusual anti‐biofouling abilities, atypical sequestration and release behaviors for guest molecules, and special mechanical properties.magnified image

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“…Such findings were consistent with the fouling mechanisms of protein or lipopolysaccharide against a hydrophobic or hydrophilic surface. [61,62] More importantly, amphiphilic crosslinked…”

Section: Anti-fouling and Fouling-release Performance Of Hbfp (I) /Pementioning

confidence: 99%

Hyperbranched Fluoropolymers and their Hybridization into Complex Amphiphilic Crosslinked Copolymer Networks

Bartels¹,

Cheng²,

Powell³

et al. 2007

Macro Chemistry & Physics

100

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“…Further, tests like SEM, TEM and XPS must be performed in vacuum, where water is removed from the sample [6][7][8][9]. Although AFM works at ambient temperature, even in liquid the surface is visualized only over a very small area [7,8,[10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…BSA is thought to compete with the polymerase for adsorption at the chip walls and, thus, to improve PCR yields [2]. The adsorption mechanism of Taq polymerase [11] and BSA protein [9,23] has been extensively studied. BSA also acts as a polymerase competitor in the inhibitor chelation [24].…”

Section: Introductionmentioning

confidence: 99%

“…In subsequent years, many more adjuvants, along with passive and active coating strategies, have been investigated [21]. Jeyachandran et al showed that BSA molecules can be adsorbed to both hydrophobic and hydrophilic surfaces [23] with differing adsorption mechanisms and rates [9]. Prakash et al systematically studied polymerase adsorption on 13 materials [11], but this study did not use actual PCR conditions, and thus the results are not necessarily translatable to PCR compatibility due to the temperature dependence of adsorption.…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory effect of common microfluidic materials on PCR outcome

Kodzius

Xiao

et al. 2012

Sensors and Actuators B: Chemical

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a b s t r a c tIn this study, we established a simple method for evaluating the PCR compatibility of various common materials employed when fabricating microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most cases, adding bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, although they noticeably interacted with the polymerase. We provide a simple method of performing PCR-compatibility testing of materials using inexpensive instrumentation that is common in molecular biology laboratories. Furthermore, our method is direct, being performed under actual PCR conditions with high temperature. Our results provide an overview of materials that are PCR-friendly for fabricating microfluidic devices. The PCR reaction, without any additives, performed best with pyrex glass, and it performed worst with PMMA or acrylic glue materials.

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“…This should form a continuous monolayer of BSA at the quartz/water interface. 44,45 Excess protein molecules were washed away with copious amounts of purified water. Next, the substrate was exposed to deep UV radiation from a mercury Pen-Ray lamp (UVP Inc., Upland, CA) in a quartz envelope.…”

Section: Resultsmentioning

confidence: 99%

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

2008

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A method has been designed for patterning supported phospholipid bilayers (SLBs) on planar substrates and inside microfluidic channels. To do this, bovine serum albumin (BSA) monolayers were formed via adsorption at the liquid/solid interface. Next, this interfacial protein film was selectively patterned by using deep UV lithography. Subsequently, SLBs could be deposited in the patterned locations by vesicle fusion. By cycling through this process several times, spatially addressed bilayer arrays could be formed with intervening protein molecules serving as twodimensional corrals. By employing this method, phospholipid bilayers containing various concentrations of ganglioside GM1 were addressed along the length of individual microfluidic channels. Therefore, the binding of GM1 with pentameric cholera toxin B (CTB) subunits could be probed. A seven-channel microfluidic device was fabricated for this purpose. Each channel was simultaneously patterned with four chemically distinct SLBs containing 0, 0.2, 0.5, and 2.0 mol % GM1, respectively. Varying concentrations of CTB were then introduced into each of the channels. With the use of total internal reflection fluorescence microscopy, it was possible to simultaneously abstract multiple equilibrium dissociation constants as a function of ligand density for the CTB-GM1 system in a single shot.Multivalent ligand-receptor interactions are ubiquitous on cell surfaces. They have a wide variety of consequences including the modulation of equilibrium dissociation constants, high receptor selectivity, and receptor clustering. 1 Multivalency can also play a direct role in signal transduction processes. 2 Examination of the underlying thermodynamics of ligandreceptor binding may lead to a greater understanding of its biological role and could provide insight into biomedical applications involving inhibitory drug design. 1,3,4 Unfortunately, high-throughput, low-protein consumption assays are not presently well enough developed in this field to afford rapid, systematic studies of binding data at two-dimensionally fluid bilayer interfaces. 5 In previous work it has been demonstrated that microfluidic devices can be designed for measuring binding affinities in multivalent systems. [5][6][7][8][9][10][11][12][13][14] In our previous setup, ligands were incorporated into supported lipid bilayers (SLBs) coated on the walls and floors of polydimethylsiloxane/glass microchannels. Linear arrays of channels were then monitored by total internal reflection fluorescence microscopy (TIRFM) 15 to obtain equilibrium dissociation constants in one-shot assays. This could be done by using the same surface chemistry in each microchannel while varying the solution concentration of the aqueous proteins. Such a setup made these assays relatively rapid, afforded high accuracy, and used only a few microliters of protein solution. Nevertheless, such platforms could be markedly improved by measuring multiple binding constants with various membrane chemistries simultaneously. This could be achie...

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A New Interpretation of Serum Albumin Surface Passivation

Cited by 122 publications

References 24 publications

Hyperbranched Fluoropolymers and their Hybridization into Complex Amphiphilic Crosslinked Copolymer Networks

Hyperbranched Fluoropolymers and their Hybridization into Complex Amphiphilic Crosslinked Copolymer Networks

Inhibitory effect of common microfluidic materials on PCR outcome

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

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