Food supply, safety and quality have become major concerns worldwide. Agri-food supply chains (ASC) possess special characteristics due to the perishability of their products and the high uncertainty of supply and demand. Furthermore, different sources of CO 2 emissions exist in an ASC due to storage, transportation, and disposal of fresh produce. Thus to ensure the sustainability of the supply chain, planning decisions have to be made with consideration of both economic and environmental aspects. This work studies the effect of changing the order quantity in a two-echelon agri-food supply chain on costs, emissions, and service level. A discrete-event simulation model is developed to include stochastic demand and lead-time, the amount of CO 2 emissions along the supply chain, service levels, and product lifetime effects. Simulation results show that reducing the order quantities can reduce costs and emissions by 27.42 % and 18.21 %; respectively, without sacrificing high service levels. Also, relying on costs or service level as sole objectives of the supply chain without consideration of emissions can result in greater economic and environmental inefficiencies in management of inventory levels.