A new labyrinthulid isolate, which solely produces n -6 docosapentaenoic acid

Kumon, Yasuyuki; Yokoyama, Rinka; Yokochi, Toshihiro; Honda, Daiske; Nakahara, Toro

doi:10.1007/s00253-003-1333-4

Cited by 23 publications

(8 citation statements)

References 27 publications

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“…Given Lohan et al (2020), we would expect that seagrass pathogen diversity to be lower in coastal waters in Alaska than Florida. We also note that Japan hosts a rich diversity of seagrass species (Miki 1934a, b;Aioi and Nakaoka 2003;Kuo et al 2006), certainly richer than Alaska, and at least four species of Zostera (Z. marina, Z. asiatica, Z. japonica, and Z. caespitosa) and at least one species of Phyllospadix (P. iwatensis) are known from Notsuke Bay and other locales on Hokkaido (Nakaoka and Aioi 2001;Kumon et al 2003). However, while pathogenic, non-pathogenic, and undetermined Labyrinthula strains were all detected in Notsuke Bay, no blight lineages were detected.…”

Section: Discussionmentioning

confidence: 77%

Metabarcoding of environmental samples suggest wide distribution of eelgrass (Zostera marina) pathogens in the north Pacific

Menning¹,

Gravley²,

Cady³

et al. 2021

MBMG

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Seagrass meadows provide important ecological services to the marine environment but are declining worldwide. Although eelgrass meadows in the north Pacific are thought to be relatively healthy, few studies have assessed the presence of known disease pathogens in these meadows. In a pilot study to test the efficacy of the methods and to provide foundational disease biodiversity data in the north Pacific, we leveraged metabarcoding of environmental DNA extracted from water, sediment, and eelgrass tissue samples collected from five widely distributed eelgrass meadows in Alaska and one in Japan and uncovered wide prevalence of two classes of pathogenic organisms – Labyrinthula zosterae and other associated strains of Labyrinthula, and the Phytophthora/Halophytophthora blight species complex – known to have caused decline in eelgrass (Zostera marina) elsewhere in the species’ global distribution. Although the distribution of these disease organisms is not well understood in the north Pacific, we uncovered the presence of at least one eelgrass pathogen at every locality sampled.

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Section: Discussionmentioning

confidence: 77%

Metabarcoding of environmental samples suggest wide distribution of eelgrass (Zostera marina) pathogens in the north Pacific

Menning¹,

Gravley²,

Cady³

et al. 2021

MBMG

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“…A was a database sequence designated Labyrinthula sp. isolated from Japan (Kumon et al 2003) with 99% identity (BLAST E-value < 10e −2000 ). Interestingly, the Laby rinthula sp.…”

Section: Genetic Identification and Phylogenetic Analysismentioning

confidence: 99%

“…Additional sequences from phylogeneticallyrelated genera were chosen from published phylogenies (Ku mon et al 2003, Tsui et al 2009, ColladoMercado et al 2010. As an outgroup we selected the chrysophyceae Ochromonas danica based on the phylogenetic ana lysis given in Kumon et al (2003). We also included several recently identified terrestrial pathogenic Labyrinthula taxa (Craven et al 2005, Douhan et al 2009).…”

Section: Sequencingmentioning

confidence: 99%

Widespread occurrence of endophytic Labyrinthula spp. in northern European eelgrass Zostera marina beds

Bockelmann¹,

Beining²,

Reusch³

2012

Mar. Ecol. Prog. Ser.

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Seagrasses worldwide are commonly infected by endophytic protists of the genus Labyrinthula. To date, the nature of the interaction of endophyte and host is not well understood. In eelgrass Zostera marina, the endophyte Labyrinthula zosterae may become virulent (pathogenic) and lead to the loss of entire seagrass beds. One of the best known examples of any marine epidemic was a series of outbreaks of the 'wasting disease' on both sides of the Atlantic in the 1930s, but smaller infestations have been reported more recently. Until now, detection of infection by Labyrinthula spp. was based on the wasting index (i.e. the relative area of leaf lesions) or microscopy, while genetic data were virtually absent. We characterized a ~1400 bp portion of the 18S small subunit rDNA in L. zosterae isolates (N = 41) from 6 northern European sites and 1 southern location (Adriatic Sea) in order to assess the identity and potential diversity of endophytic protists. Because there are indications that low salinity impedes Labyrinthula growth, sampling sites included a wide range of salinities from 5 to 34 psu. A search against the non-redundant GenBank database revealed that most isolates are 99% similar to the only L. zosterae 18S sequence available from the database at all but the Finnish site (salinity values 5 to 7 psu). At this site, a different Labyrinthula species occurred, which was also found in fully marine Wadden Sea cultures. A third species was detected in Skagerrak, south-western Baltic and North Sea samples (20 to 25 psu). We conclude that L. zosterae is widespread among northern European eelgrass sites across wide ranges of salinity. KEY WORDS: Labyrinthula infection · Eelgrass · 18S sequence analysis · Salinity · Wasting diseaseResale or republication not permitted without written consent of the publisher

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“…AN‐1565 (AB022105) and Labyrinthula sp. L59 (AB095092), known to produce n‐6 docosapentaenoic acid (n‐6 DPA), a long chain polyunsaturated fatty acid (Kumon et al , 2003).…”

Section: Resultsmentioning

confidence: 99%

An in situ method for cultivating microorganisms using a double encapsulation technique

Ben‐Dov

Kramarsky‐Winter

Kushmaro

2009

FEMS Microbiology Ecology

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The lack of cultured microorganisms represents a bottleneck for advancement in microbiology. The development of novel culturing techniques is, therefore, a crucial step in our understanding of microbial diversity in general, and the role of such diversity in the environment, in particular. This study presents an innovative method for cultivating microorganisms by encapsulating them within agar spheres, which are then encased in a polysulfonic polymeric membrane and incubated in a simulated or natural environment. This method stimulates growth of the entrapped microorganisms by allowing them access to essential nutrients and cues from the environment. It allows for the discovery of microorganisms from dilutions that are 10-100-fold greater than possible with conventional plating techniques. Analysis of microorganisms grown in such spheres incubated in and on a number of different substrates yielded numerous novel ribotypes. For example, spheres incubated on the mucus surface of a Fungiid coral yielded numerous ribotypes, with only 50% sharing similarity (85-96%) to previously identified microorganisms. This suggests that many of the species represent novel ribotypes. Hence, the technique reported here advances our ability to retrieve and successfully culture microorganisms and provides an innovative tool to access unknown microbial diversity.

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A new labyrinthulid isolate, which solely produces n -6 docosapentaenoic acid

Cited by 23 publications

References 27 publications

Metabarcoding of environmental samples suggest wide distribution of eelgrass (Zostera marina) pathogens in the north Pacific

Metabarcoding of environmental samples suggest wide distribution of eelgrass (Zostera marina) pathogens in the north Pacific

Widespread occurrence of endophytic Labyrinthula spp. in northern European eelgrass Zostera marina beds

An in situ method for cultivating microorganisms using a double encapsulation technique

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