A new laccase-catalyzed domino process and its application to the efficient synthesis of 2-aryl-1H-benzimidazoles

Optimization of Laccase Fermentation and Evaluation of Kinetic and Thermodynamic Parameters of a Partially Purified Laccase Produced byDaedalea flavida

“…[48] DH Ã of laccase has been calculated using the Eyring equation as per the method described earlier. Equation (5) gives the values of DG Ã . DS Ã has been calculated using Eq.…”

“…Thus, this enzyme has wide applications in paper manufacture, food processing, bioremediation, biosensors, fuel cells, organic synthesis, and so on. [1][2][3][4][5] For practical applications of laccase, an efficient production system is necessary along with the thermodynamic and kinetic information of the enzyme produced in optimal conditions. O 8), which is a partial modification of the composition reported by Chander et al [2] A 10-mL aliquot of seed culture containing uniform pellets ($0.02 g dry cell weight) was used in all the experiments.…”

Oxidation of o‐phenylenediamine to 2,3‐diaminophenazine in the presence of cubic ferrites MFe2O4 (M = Mn, Co, Ni, Zn) and the application in colorimetric detection of H2O2

“…[9] The oxidation of OPD to DAP is of great importance in immunoassay determination of enzyme-catalyzed reactions such as oxygenase/oxidases and peroxidases. For instance, this oxidation reaction has been used for measuring the catalytic activity of the multi-copper containing oxidase (Laccase) [10] and the horseradish peroxidase (HRP). [11] Zhang et al have developed a new method for the determination of hemoglobin (Hb) based on its enzymatic activity for the oxidation of OPD to DAP.…”

2,3-Diaminophenazine

“…Similar to HRP, laccase (EC 1.10.3.2), a copper-containing polyphenol oxidases produced dominantly in plants and microorganisms, also carries the ability to catalyze the oxidation of phenols and amines [5]. Recently, the potential of laccase-catalyzed polymerization of OP has been demonstrated [6] but the main products from laccase/OP reaction still needs to be further characterized. The difference between HRP and laccase-catalyzed reactions is that laccase in acetic buffer needs oxygen as an oxidant while HRP requires the involvement of hydrogen peroxide ( Figure 1).…”