A new liver-tumor model in the rat

Nagel, Judith; Kort, Will J.

doi:10.1007/bf00686488

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…In the literature four different routes have been reported as potential ways to induce, experimentally, liver neoplastic disease: tumour implantation in the liver [9,23,29,30,31,33]; neoplastic cell infusion through the portal route [10,11,12,13,14,34]; liver metastasis development after intracaecal implantation of tumour [35,36]; and chemical liver tumour induction [37].…”

Section: Discussionmentioning

confidence: 99%

An experimental approach of the Doppler perfusion index of the liver in detecting occult hepatic metastases: histological findings related to the hemodynamic measurements in Wistar rats

et al. 2000

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Image-directed colour Doppler sonography has been successfully introduced for the detection of hepatic haemodynamic changes in the presence of liver metastases. The aim of our study was to correlate these haemodynamic changes with the liver histology at the time of measurement. We experimentally induced liver metastases in 30 male Wistar rats by inoculating Walker 256 tumour subcutaneously. The animals were assigned into three groups of ten and were studied sonographically at 4, 7 and 15 days after tumour implantation. Another group of ten normal animals were used as controls. Portal vein and hepatic artery measurements included resistance index (PVRI, HARI) and flow volume (PVFV, HAFV). Doppler perfusion index (DPI) of the liver was calculated as the ratio of HAFV/PVFV + HAFV. Liver histology followed each Doppler measurement. Metastases were first encountered on day 4, as small groups of cells in the connective tissue of the porta hepatis and the portal triads without apparent vascular association. Distinct elevation of HAFV and DPI was recorded in comparison with the controls (p = 0.0004 and p = 0.0005, respectively). PVFV reduction was subtle. Up to day 15 there were no significant changes in the measurements. Our data suggest that HAFV and DPI can efficiently detect early liver metastases and this is in accordance with existing clinical reports. Haemodynamic changes seem to originate from the early non-vascular phase of the metastases.

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Section: Discussionmentioning

confidence: 99%

An experimental approach of the Doppler perfusion index of the liver in detecting occult hepatic metastases: histological findings related to the hemodynamic measurements in Wistar rats

et al. 2000

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“…After collection of livers in anaesthetised mice, they were then killed by exsanguinations through division of the abdominal aorta. The difference in tumour growth between groups was confirmed using the liver to body weight ratio as a surrogate for tumour burden [27].…”

Section: Experimental Designmentioning

confidence: 85%

Tumour growth stimulation following partial hepatectomy in mice is associated with increased upregulation of c-Met

Harun

Costa

Christophi

2013

Clin Exp Metastasis

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Hepatic resection is the preferred option for curative treatment of colorectal liver metastasis (CLM). However, this is associated with significant recurrence rates in both hepatic and extrahepatic sites. The upregulation of growth factors required for liver regeneration after resection is thought to stimulate the growth of micrometastases. The current study describes temporal changes in the expression of hepatocyte growth factor receptor (c-Met), epidermal growth factor receptor (EGFR) and insulin growth factor I receptor (IGF-IR) in an orthotopic mouse model of liver resection and tumour induction. Mice underwent 70% hepatectomy and induction of liver metastases through intrasplenic injection of colorectal cancer cells. Control groups included sham-operated mice and 70% hepatectomy alone. The expression levels of liver and tumour c-Met, EGFR and IGF-IR were quantified by quantitative RT-PCR at different time points. 70% liver resection stimulates tumour growth; increases the expression of c-Met within established tumours and surrounding liver parenchyma; downregulates EGFR expression and increases IGF-IR expression within the liver parenchyma. In conclusion, we demonstrate in our mouse model that major hepatectomy stimulates engraftment and growth of CLM and that this effect is probably due to the upregulation of c-Met as a result of the liver regeneration process. Liver IGF-IR may also contribute to this phenomenon through a paracrine effect on tumour growth. This study provides support for the role of c-Met in the stimulation of tumour growth after resection possibly through the promotion of tumour cell proliferation.

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“…Modelos animais de tumor hepático vêem sendo utilizados para o desenvolvimento e aprimoramento de meios diagnósticos por imagem 12,13,14,15 e para testes de novas terapias farmacológicas 16,17 , cirúrgicas e meios físicos de hipertermia e hipotermia.…”

Section: Discussionunclassified

Quimioembolização transarterial hepática: modelo experimental de tumor em ratos

Zarur¹,

Barreto

Diogenes

et al. 2004

Acta Cir. Bras.

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OBJETIVO: Estabelecer um modelo de tumor no fígado de ratos para estudo do comportamento tumoral e avaliar o uso da quimiembolização transarterial. MÉTODOS: Utilizou-se oitenta e oito ratos Wistar, fêmeas, adultos, pesando entre 175- 284 g . Realizado incisão abdominal de 3 cm e implantou-se o carcinossarcoma de Walker 256 no lóbulo esquerdo do fígado. Dividiu-se em três grupos que receberam respectivamente 100x 10³ , 200x 10³ e 300x 10³ células tumorais, avaliado a pega do tumor e a sobrevida. Em outro grupo de experimento com 39 animais inoculados com tumor de Walker foi avaliado a sobrevida dos animais após infusão do 5-Flourouracil (5-FU) por via intra-peritoneal e intra-arterial. RESULTADOS: O implante do carcinossarcoma de Walker no fígado de ratos apresentou desenvolvimento de 100 %, teve um crescimento rápido e desenvolvimento de metástases tardiamente, levando os animais ao óbito entre o sétimo e décimo quinto dia. A quimiembolização transarterial é possível de ser realizada experimentalmente. O uso do 5-FU aumentou a sobrevida em comparação ao grupo controle. CONCLUSÃO: O modelo de implante do tumor de Walker no fígado de ratos é eficiente, de fácil reprodutibilidade, e sobrevida média de 9,96±0.3 dias. A quimioterapia transarterial hepática pode ser realizada experimentalmente para avaliar diversas drogas.

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A new liver-tumor model in the rat

Cited by 4 publications

References 6 publications

An experimental approach of the Doppler perfusion index of the liver in detecting occult hepatic metastases: histological findings related to the hemodynamic measurements in Wistar rats

An experimental approach of the Doppler perfusion index of the liver in detecting occult hepatic metastases: histological findings related to the hemodynamic measurements in Wistar rats

Tumour growth stimulation following partial hepatectomy in mice is associated with increased upregulation of c-Met

Quimioembolização transarterial hepática: modelo experimental de tumor em ratos

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