According to the PVT high pressure physical parameters of oil and gas, Using the PVTI in reservoir numerical simulation software Eclipse to determine the phase characteristics of the fluid, fitting the basic parameters and building component model, using the GEM modular program of CMG to calculate the oil displacement efficiency under different inject pressure, according to oil displacement efficiency and injection pressure relationship curve presents two lines before and after the inflection point, regressing the equation of two lines, and the intersection of the pressure be determined the minimum miscibility pressure of carbon dioxide-crude oil; in order to confirm the results, at the same time using slim tube experiment method and empirical formula methods to determine the minimum miscibility pressure of carbon dioxide-crude oil, and the slim tube experiment should be treated as the reference standard, Calculating the relative error of CMG method and the empirical formula method which are compared with the measured value of slim tube method. The results show that when the reservoir temperature are 90℃and 108℃, the carbon dioxide-crude oil minimum miscibility pressure of Shu 101 block in Yushulin oilfield is predicted by CMG, comparing with measured value of slim tube method, the relative error are 1.54% and 0.72%, the average relative error is 1.13%, which is less than the commonly used empirical formula of relative error average value, showing that carbon dioxide-oil minimum miscibility pressure is accurate and reliable by CMG.