A New Lung Cancer Biomarker—A Preliminary Report

Masilamani, V.; Vijmasi, Trinka; Salhi, Mohamad Al; Elangovan, M.; Raghavan, Vijaya; Diab, Abdul Rahman; Hajjar, Waseem; Ainia, Mohammad; Al-Mustafa, Abdulaziz; Al-Nachawati, H.

doi:10.1089/pho.2009.2615

Cited by 10 publications

(10 citation statements)

References 20 publications

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“…Figure 2 gives a representation of scatter plot of ratio parameters R 1 (a measure of porphyrin concentration in the erythrocyte, as shown in) 10 11 12 13 14 for the control and smoker sets. It was observed that most of the R 1 values for the smoker group were less than the cutoff value, R 1 = 1 (arbitrarily chosen).…”

Section: Resultsmentioning

confidence: 99%

“…Out of a large number of such bio-molecules such as proteins or amino acids, only a dozen of them fluorescence in the range of 200 to 800 nm. The relative proportion of such fluorescent molecules acts as biomarkers of a certain set of well-defined diseases 9 10 11 12 13 14 .…”

Section: Methodsmentioning

confidence: 99%

“…These molecules include nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), porphyrin, etc., which have partially overlapping emission profiles in complex systems such as the blood plasma. It is important to emphasize that FES and SES are variants of fluorescence spectroscopy 9 10 11 and that both spectra can be considered to be optical analogues of X-ray radiography and computed tomography (CT) scan 10 11 12 13 14 .…”

Section: Methodsmentioning

confidence: 99%

“…Similar studies have focused on the detection of malignancy of lung, liver, etc. 11 12 (and inherited blood disorders, such as thalassemia and sickle cell anemia) 13 from the spectral features of blood and urine 14 . The fluorescent spectral technique can be used for molecular diagnosis, and the present study can be considered as a logical extension of the spectral technique of blood components for monitoring smoking-induced damage on erythrocytes.…”

mentioning

confidence: 99%

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Smoking Induced Hemolysis: Spectral and microscopic investigations

Masilamani

Alzahrani

Devanesan

et al. 2016

Sci Rep

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Smoking is one of the major causes of lifestyle associated mortality and morbidity such as cancer of the oral cavity and lungs, and also cardiovascular diseases. In this study, we have provided evidences for the smoking-induced hemolysis using two methods: spectra of blood components and atomic force microscopic analysis of surface morphology. A total of 62 subjects (control = 31; smoker = 31: 21 male; 10 female in each set) were considered for the study. The findings indicate that smoking leads to potholes on the surface, swelling of shape, rupturing of erythrocytes, removal of hematoporphyrin and flushing into the plasma as metabolites of the erythrocyte. The overall morphology of the erythrocytes of the smoker group appears more like a Mexican hat. The mean surface roughness was 5.5 ± 3 nm for the smoker group, but 1.2 ± 0.2 nm for the control group. Such damages might help the toxins, (CO, peroxidants, aldehydes etc.,) to gain easy access and get strongly absorbed by the hemoglobin, leading to enhanced rates of hemolysis as shown by the spectral features of metabolites. This indicates that the average life span of the smoker’s erythrocytes is significantly less than that of the control group.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Smoking Induced Hemolysis: Spectral and microscopic investigations

Masilamani

Alzahrani

Devanesan

et al. 2016

Sci Rep

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“…These two signals are fingerprints of biomolecules that are commonly found in tissue or fluid. The nature, quality, and quantity of biomolecules can be correlated to the specific disease of the subjects, as can be shown by the large number of studies on cancer tissues and the blood of inherited disorders [ 7 , 8 , 9 , 10 , 11 , 12 ]. The fluorescent spectral technique can be regarded as a new technique of molecular diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Impact of Diabetes Mellitus on Human Erythrocytes: Atomic Force Microscopy and Spectral Investigations

AlSalhi

Devanesan

Alzahrani

et al. 2018

IJERPH

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Diabetes mellitus (DM) is a common metabolic disease indicated by high sugar levels in the blood over a prolonged period. When left untreated, it can lead to long-term complications, such as cardiovascular disease, stroke, and diabetic retinopathy or foot ulcers. Approximately 415 million people (about 8.3% of the world’s population) had diabetes worldwide in 2015, with 90% of the cases classified as Type 2 DM, which is caused by insulin resistance that arises mostly from being overweight and from a lack of exercise. DM affects every part of the body, including the erythrocytes. The aim of the present report is to gain insight into the damage done to the erythrocytes of patients classified with pre-diabetes and diabetes (plenty are found in the Kingdom of Saudi Arabia, a country where young people encompass a large segment of the population). The study presents results on the morphological analysis of erythrocytes by atomic force microscopy (AFM) and molecular investigations by fluorescence spectroscopy (FS). Our results indicate significant differences (in the morphology, size, and hemolytic end products) between the erythrocytes of diabetic patients (HbA1C, glycated hemoglobin, levels of 8–10%) and normal controls. It is well-known that DM and smoking are two major contributory factors for cardiovascular diseases (CVDs), and our observations presented in this study suggest that diabetes plays a relatively less damaging role than smoking for CVD.

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Autofluorescence of blood and its application in biomedical and clinical research

Shrirao

Schloss

Fritz

et al. 2021

Biotech & Bioengineering

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Autofluorescence of blood has been explored as a label free approach for detection of cell types, as well as for diagnosis and detection of infection, cancer, and other diseases.Although blood autofluorescence is used to indicate the presence of several physiological abnormalities with high sensitivity, it often lacks disease specificity due to use of a limited number of fluorophores in the detection of several abnormal conditions. In addition, the measurement of autofluorescence is sensitive to the type of sample, sample preparation, and spectroscopy method used for the measurement. Therefore, while current blood autofluorescence detection approaches may not be suitable for primary clinical diagnosis, it certainly has tremendous potential in developing methods for large scale screening that can identify high risk groups for further diagnosis using highly specific diagnostic tests. This review discusses the source of blood autofluorescence, the role of spectroscopy methods, and various applications that have used autofluorescence of blood, to explore the potential of blood autofluorescence in biomedical research and clinical applications.

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A New Lung Cancer Biomarker—A Preliminary Report

Cited by 10 publications

References 20 publications

Smoking Induced Hemolysis: Spectral and microscopic investigations

Smoking Induced Hemolysis: Spectral and microscopic investigations

Impact of Diabetes Mellitus on Human Erythrocytes: Atomic Force Microscopy and Spectral Investigations

Autofluorescence of blood and its application in biomedical and clinical research

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