A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor

Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells

“…Cells cultivated in AEM and EXCELL MDCK achieved maximal densities of 5.0×10 6 cells/mL, with a mean viability of 76 and 80 %, respectively. The mean viability determined for SMIF8 cultures was slightly higher (83 %), and the maximal cell density was lower (2.7×10 6 cells/mL), which is in accordance with the values reported before (Lohr et al 2010). …”

“…DKCre and MDCK cells were cultivated in Dulbecco's modified Eagle's medium (DMEM; Life Technologies Ltd, Paisley, UK) supplemented with 10 % (v/ v) of fetal bovine serum (FBS; Life Technologies Ltd, Paisley, UK) and maintained at 37°C in humidified atmosphere containing 5 % of CO 2 . MDCK.SUS2, a new suspension cell line derived from adherent MDCK cells as described before (Lohr et al 2010), was evaluated in growth and CAV-2 production studies. MDCK.SUS2 cells were subcultured twice a week in Adenovirus Expression Medium (AEM; Life Technologies Ltd, Paisley, UK) supplemented with 4 mM of L-glutamine (Life Technologies Ltd, Paisley, UK).…”

“…MDCK.SUS2 suspension cultures are characterized by cell aggregation (Lohr et al 2010). In this study, we compare the size and shape of the MDCK.SUS2 cell aggregates for the three chosen culture media (Fig.…”

“…The advantages of using serum-free media for the industrial production of biopharmaceuticals have been intensively discussed and include the reduction of contamination risks with pathogenic agents present in the serum, the reduction of production costs, and the ease in downstream processing (Falkner et al 2006). The establishment of two suspension-adapted MDCK cell lines, MDCK.SUS2 and MDCK-SFS, has been recently reported (Lohr et al 2010;van Wielink et al 2011), and their use as cell substrate for influenza virus production is also described.…”

Cell culture-based influenza vaccines: A necessary and indispensable investment for the future

“…The cells can be easily adapted to and be grown in serum-free media, and in suspension, as well as on various microcarriers maintained under various bioreactor conditions. 93,[101][102][103][104][105][106][107][108] Subclones of MDCK cells adopted to grow in suspension and support robust virus production have also been described, 91,108,109 although adherent MDCK cells appear to support more robust virus production than suspension MDCK cells. 110 Influenza vaccines derived from MDCK cells are also safe and immunogenic.…”