A new membrane antigen revealed by monoclonal antibodies is associated with motoneuron axonal pathways

Tanaka, Hideaki; Agata, Akemi; Obata, Kunihiko

doi:10.1016/0012-1606(89)90238-8

Cited by 46 publications

(30 citation statements)

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“…9C) despite the reported differential expression of members of the trk family of receptors in the chicken retina , we tested whether p75 was distributed in the same layer in which the exogenous BDNF accumulated. Immunocytochemistry with a monoclonal antibody to p75 (Tanaka et al, 1989;von Bartheld et al, 1995) showed distinct patterns of p75 label in the retina at E14 -E16. Most neuropil label was in the IPL with punctate label, particularly in the outer third of the IPL (Fig.…”

Section: Distribution Of P75 Receptor Protein In the Retinamentioning

confidence: 94%

“…Sections were rinsed three times and incubated with a biotinylated goat anti-rabbit antibody diluted in blocking serum. For immunostaining with monoclonal p75 antibody (Tanaka et al, 1989;von Bartheld et al, 1995), a biotinylated horse anti-mouse antibody was used. After three washes ABC reagent was applied to the sections for 30 min, rinsed three times, and preincubated for 10 min in 0.1% diaminobenzidine (DAB) in 0.04% nickel in TBS (100 mM Tris, pH 7.4, and 150 mM NaC l).…”

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confidence: 99%

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Contributions of the Optic Tectum and the Retina as Sources of Brain-Derived Neurotrophic Factor for Retinal Ganglion Cells in the Chick Embryo

1998

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Retinal ganglion cells (RGC) are supported by brain-derived neurotrophic factor (BDNF), but it is not known if BDNF acts as a target-derived factor or as an afferent or autocrine trophic factor. Here we demonstrate that BDNF mRNA is expressed in the retinorecipient layer of the chick optic tectum as well as in the inner nuclear layer and ganglion cell layer of the retina. Amacrine cells rather than RGC were the main source of BDNF mRNA in the ganglion cell layer, as determined by in situ hybridization that was combined with retrograde labeling of RGC and destruction of RGC by optic stalk transection, followed by quantitative RT-PCR. Cells in the ganglion cell layer as well as the retinorecipient layers of the optic tectum were BDNF-immunolabeled. After injections into the tectum, radioiodinated BDNF was transported to the retina where autoradiographic label accumulated in the inner plexiform and ganglion cell layers. After intraocular injection, iodinated BDNF accumulated in these same retinal layers and correlated with the distribution of p75 neurotrophin receptor protein. The majority of cross-linked receptor-bound BDNF in the retina immunoprecipitated with p75 antibodies. No difference in the intensity of BDNF immunolabel was observed in the experimental retina or tectum after optic stalk transection, indicating that most of the BDNF in the RGC was not derived from the optic tectum. These data indicate that a substantial fraction of the BDNF in the ganglion cell layer is derived from local sources, afferents within the retina, rather than from the optic tectum via retrograde transport.

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Section: Distribution Of P75 Receptor Protein In the Retinamentioning

confidence: 94%

mentioning

confidence: 99%

Contributions of the Optic Tectum and the Retina as Sources of Brain-Derived Neurotrophic Factor for Retinal Ganglion Cells in the Chick Embryo

1998

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“…Neither of the trk antibodies labeled a distinct band of neuropil in the retinorecipient tectal layers. Immunocytochemistry with a monoclonal antibody specific for chicken p75 (Tanaka et al, 1989;von Bartheld et al, 1995) showed that strong p75 immunoreactivity was present in the neuropil-containing retinotectal fibers and terminals (tectal layers SO and SGFSa-d, Fig. 8 D).…”

Section: Localization Of Neurotrophin Receptors In the Retinotectal Pmentioning

confidence: 97%

Expression of Neurotrophin-3 (NT-3) and Anterograde Axonal Transport of Endogenous NT-3 by Retinal Ganglion Cells in Chick Embryos

Bartheld

Butowt

2000

J. Neurosci.

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Anterograde axonal transport of neurotrophins has been demonstrated recently, but to date such transport has only been shown for brain-derived neurotrophic factor and no other endogenous neurotrophin. Endogenous neurotrophin-3 (NT-3) protein is present in the ganglion cell layer of the chicken retina, as well as the superficial layers of the optic tectum. NT-3 immunolabel in these tectal layers is largely reduced or abolished after treatment of the eye with colchicine or monensin, demonstrating that endogenous NT-3 is transported to the optic tectum by retinal ganglion cells (RGCs). Reverse transcription-PCR analysis of RGCs purified to 100% shows that RGCs, but not tectal cells, express NT-3 mRNA. Blockade of the intercellular transfer of NT-3 within the retina does not reduce the anterograde transport of endogenous NT-3 to the tectum, indicating that a major fraction of the anterogradely transported NT-3 is produced by RGCs rather than taken up from other retinal cells. Immunolabel for the neurotrophin receptor p75, but not trkB or trkC, in the superficial tectum coincides with the NT-3 label. The p75 label in the neuropil of superficial tectal layers is largely reduced or eliminated by injection of monensin in the eye, indicating that p75 protein is exported along RGC axons to the retinotectal terminals and may act as a neurotrophin carrier. These results show that NT-3 is produced by RGCs and that some of this NT-3 is transported anterogradely along the axons to the superficial layers of the tectum, possibly to regulate the survival, synapse formation, or dendritic growth of tectal neurons. Key words: anterograde transport; NT-3; BDNF; retina; optic tectum; p75 neurotrophin receptor; neurotrophic factor; visual system; RT-PCRNeurotrophins are well known as target-derived, retrograde survival-promoting molecules that require the transport of the neurotrophic factor from the axon terminus to the cell body (Hendry et al., 1974;Johnson et al., 1978;Purves, 1988;Barde, 1989;Oppenheim, 1996). Neurotrophins are also important regulators of neuronal differentiation and synaptic plasticity (Snider, 1994;Thoenen, 1995). Recent studies have shown that neurotrophins can be transported anterogradely along axons (von Bartheld et al., 1996a;Z hou and Rush, 1996;Altar et al., 1997;Conner et al., , 1998Heymach and Barres, 1997;Johnson et al., 1997;Michael et al., 1997;Smith et al., 1997;Yan et al., 1997;Altar and DiStefano, 1998). Such anterograde transport may f unction to provide trophic support from afferents (Linden, 1994;von Bartheld et al., 1996a;Altar et al., 1997), to mediate fast, local effects at synaptic sites (L ohof et al., 1993;Kang and Schuman, 1995;Thoenen, 1995;Berninger and Poo, 1996), or to regulate dendritic growth, neuronal cytoarchitecture, and phenotypes (Purves, 1988;Altar et al., 1997;McAllister et al., 1999). Brainderived neurotrophic factor (BDNF) is stored in axon terminals Fawcett et al., 1997;Michael et al., 1997) and may be released into the synaptic cleft to activate postsynaptic neurotrophin ...

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“…At the level of the hindlimb, motor axons of the LMC exit the neural tube beginning at E2.75 (HH18) through the ventral roots and extend ventrolaterally through the anterior portion of the adjacent sclerotome into the somatopleure at the base of the hindlimb (Hollyday 1980a;Keynes and Stern, 1984;Tosney, 1988;Tanaka et al, 1989;Wang and Scott, 2000;Turney et al, 2003). Neural crest cells are present with the dorsal portion of the anterior sclerotome at E2.75 (HH18; Spence and Poole, 1994).…”

Section: Nervous Development Of the Japanese Quail Hindlimbmentioning

confidence: 99%

“…Within the LMC itself, cell bodies in the medial portion of the LMC provide innervation to the ventral portion of the hindlimb while cell bodies in the lateral portion of the LMC supply the dorsal portion of the hindlimb (Tosney and Landmesser, 1985;Tyrrell et al, 1990;Tosney et al, 1995;Turney et al, 2003). By E3.5 (HH21), some motor neurites have reached the hindlimb plexus region where they undergo extensive axon sorting with axons from neighboring spinal cord segments (Tosney and Landmesser, 1985;Tanaka et al, 1989;Wang and Scott, 1999). Axons of the DRG arise through the ventrolateral migration of cells from the neural crest and come to occupy a position corresponding to the rostral half of the particular somitic level (Teillet et al, 1987).…”

Section: Nervous Development Of the Japanese Quail Hindlimbmentioning

confidence: 99%

Neurovascular Anatomy of the Embryonic Quail Hindlimb

Bentley

Poole

2009

The Anatomical Record

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Blood vessel and nerve development in the vertebrate embryo possess certain similarities in pattern and molecular guidance cues. To study the specific influence of shared guidance molecules on nervous and vascular development, an understanding of the normal neurovascular anatomy must be in place. The present study documents the pattern of nervous and vascular development in the Japanese quail hindlimb using immunohistochemistry and fluorescently labeled intravital injection combined with confocal and epifluorescent microscopy. The developmental patterns of major nerves and blood vessels of embryonic hindlimbs between stages E2.75 (HH18) and E6.0 (HH29) are described. By E2.75, the dorsal aortae have begun to fuse into a single vessel at the level of the hindlimb, and have completely fused by E3 (HH20). The posterior cardinal vein is formed at the level of the hindlimb by E3, as is the main artery of the early hindlimb, the ischiadic artery, as an offshoot of the dorsal aorta. Our data suggest that eight spinal segments, versus seven as reported by others (Tanaka and Landmesser, 1986a;Tyrrell et al., 1990), contribute to innervation of the quail hindlimb. Lumbosacral neurites reach the plexus region by E3.5 (HH21 & 22), pause for $24 hr, and then enter the hindlimb along with the ischiadic and crural arteries through shared foramina in the pelvic anlage. The degree of anterior-posterior spatial congruency between major nerves and blood vessels of the quail hindlimb was found to be highest medial to the pelvic girdle precursor, versus in the hindlimb proper. Anat Rec, 292:1559Rec, 292: -1568Rec, 292: , 2009. V V C 2009 Wiley-Liss, Inc.

show abstract

A new membrane antigen revealed by monoclonal antibodies is associated with motoneuron axonal pathways

Cited by 46 publications

References 45 publications

Contributions of the Optic Tectum and the Retina as Sources of Brain-Derived Neurotrophic Factor for Retinal Ganglion Cells in the Chick Embryo

Contributions of the Optic Tectum and the Retina as Sources of Brain-Derived Neurotrophic Factor for Retinal Ganglion Cells in the Chick Embryo

Expression of Neurotrophin-3 (NT-3) and Anterograde Axonal Transport of Endogenous NT-3 by Retinal Ganglion Cells in Chick Embryos

Neurovascular Anatomy of the Embryonic Quail Hindlimb

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