A New Membrane-bound Cytochrome c Works as an Electron Donor to the Photosynthetic Reaction Center Complex in the Purple Bacterium, Rhodovulum sulfidophilum

Kimura, Yasuaki; Alric, Jean; Vermeglio, André; Masuda, Shinji; Hagiwara, Yuuki; Matsuura, Kiyotaka; Shimada, Keizo; Nagashima, Kenji V. P.

doi:10.1074/jbc.m604452200

Cited by 7 publications

(3 citation statements)

References 37 publications

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“…Samples were processed as previously described ( Kimura et al., 2007 ), with the exception that 4% paraformaldehyde and 2 mmol/L CaCl 2 were used in the primary fixative and 2% OsO 4 in the secondary fixative. Images were acquired using the Orius CDD SC1000 camera (Gatan, Oxon, UK).…”

Section: Methodsmentioning

confidence: 99%

Cutaneous Nod2 Expression Regulates the Skin Microbiome and Wound Healing in a Murine Model

Williams

Crompton

Thomason

et al. 2017

Journal of Investigative Dermatology

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The skin microbiome exists in dynamic equilibrium with the host, but when the skin is compromised, bacteria can colonize the wound and impair wound healing. Thus, the interplay between normal skin microbial interactions versus pathogenic microbial interactions in wound repair is important. Bacteria are recognized by innate host pattern recognition receptors, and we previously showed an important role for the pattern recognition receptor NOD2 in skin wound repair. NOD2 is implicated in changes in the composition of the intestinal microbiota in Crohn’s disease, but its role on skin microbiota is unknown. Nod2-deficient (Nod2–/–) mice had an inherently altered skin microbiome compared with wild-type controls. Furthermore, we found that Nod2–/– skin microbiome dominated and caused impaired healing, shown in cross-fostering experiments of wild-type pups with Nod2–/– pups, which then acquired altered cutaneous bacteria and delayed healing. High-throughput sequencing and quantitative real-time PCR showed a significant compositional shift, specifically in the genus Pseudomonas in Nod2–/– mice. To confirm whether Pseudomonas species directly impair wound healing, wild-type mice were infected with Pseudomonas aeruginosa biofilms and, akin to Nod2–/– mice, were found to exhibit a significant delay in wound repair. Collectively, these studies show the importance of the microbial communities in skin wound healing outcome.

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Section: Methodsmentioning

confidence: 99%

Cutaneous Nod2 Expression Regulates the Skin Microbiome and Wound Healing in a Murine Model

Williams

Crompton

Thomason

et al. 2017

Journal of Investigative Dermatology

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“…On the other hand, we do not exclude a possibility that membrane-anchored cyt c 4 works in growing cells. Our previous study on the marine purple non-sulfur bacterium R. sulfidophilum showed that the membrane-bound cyt c 2m is converted into the soluble form, cyt c 549 , by an unknown proteolytic reaction when the cell was aged . If similar conversion occurs on R. gelatinosus cyt c 4 , the rate of electron transfer to the RC may be changed according to the cell growth conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Some species among the R-subclass have membrane-bound cytochromes c, which show high sequence similarities to cytochrome c 2 but possess a hydrophobic anchor stretch at the N-terminal region. These cytochromes, referred to as cytochrome c y or c 2m , act as efficient electron donors to the RC in addition to the water-soluble cytochrome c 2 in Rhodobacter capsulatus (2) and Rhodovulum sulfidophilum (3).…”

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confidence: 99%

Cytochrome c₄ Can Be Involved in the Photosynthetic Electron Transfer System in the Purple Bacterium Rubrivivax gelatinosus

et al. 2009

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Three periplasmic electron carriers, HiPIP and two cytochromes c 8 with low- and high-midpoint potentials, are present in the purple photosynthetic bacterium Rubrivivax gelatinosus. Comparison of the growth rates of mutants lacking one, two, or all three electron carrier proteins showed that HiPIP is the main electron donor to the photochemical reaction center and that high-potential cytochrome c 8 plays a subsidiary role in the electron donation in photosynthetically growing cells. However, the triple deletion mutant was still capable of photosynthetic growth, indicating that another electron donor could be present. A new soluble cytochrome c, which can reduce the photooxidized reaction center in vitro, was purified. Based on amino acid sequence comparisons to known cytochromes, this cytochrome was identified as a diheme cytochrome c of the family of cytochromes c 4. The quadruple mutant lacking this cytochrome and three other electron carriers showed about three times slower growth than the triple mutant under photosynthetic growth conditions. In conclusion, cytochrome c 4 can function as a physiological electron carrier in the photosynthetic electron transport chain in R. gelatinosus.

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Functional Coupling Between Reaction Centers and Cytochrome bc 1 Complexes

Lavergne

Vermeglio

Joliot

2009

The Purple Phototrophic Bacteria

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A New Membrane-bound Cytochrome c Works as an Electron Donor to the Photosynthetic Reaction Center Complex in the Purple Bacterium, Rhodovulum sulfidophilum

Cited by 7 publications

References 37 publications

Cutaneous Nod2 Expression Regulates the Skin Microbiome and Wound Healing in a Murine Model

Cutaneous Nod2 Expression Regulates the Skin Microbiome and Wound Healing in a Murine Model

Cytochrome c₄ Can Be Involved in the Photosynthetic Electron Transfer System in the Purple Bacterium Rubrivivax gelatinosus

Functional Coupling Between Reaction Centers and Cytochrome bc 1 Complexes

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A New Membrane-bound Cytochrome c Works as an Electron Donor to the Photosynthetic Reaction Center Complex in the Purple Bacterium, Rhodovulum sulfidophilum

Cited by 7 publications

References 37 publications

Cutaneous Nod2 Expression Regulates the Skin Microbiome and Wound Healing in a Murine Model

Cutaneous Nod2 Expression Regulates the Skin Microbiome and Wound Healing in a Murine Model

Cytochrome c4 Can Be Involved in the Photosynthetic Electron Transfer System in the Purple Bacterium Rubrivivax gelatinosus

Functional Coupling Between Reaction Centers and Cytochrome bc 1 Complexes

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Cytochrome c₄ Can Be Involved in the Photosynthetic Electron Transfer System in the Purple Bacterium Rubrivivax gelatinosus