A new membraneless device for preparative disc electrophoresis permitting concentrated elution

Welzen, H. Van; Zuidweg, M.H.J.

doi:10.1016/0003-2697(74)90038-4

Cited by 6 publications

(1 citation statement)

References 22 publications

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“…The major challenge in implementing EFGF is the creation of a separation channel with the required EFG. EFGs have been constructed based on changing cross sections, ,− concentration gradients, ,, electrode arrays, and temperature gradients …”

mentioning

confidence: 99%

Electric Field Gradient Focusing of Proteins Based on Shaped Ionically Conductive Acrylic Polymer

Humble

Kelly²,

Woolley³

et al. 2004

Anal. Chem.

110

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Electric field gradient focusing (EFGF) is a separation technique that uses an electric field gradient and an opposing hydrodynamic flow to separate and concentrate charged analytes. This work describes miniaturized EFGF devices that are used for protein analysis. These devices employ a unique ionically conductive polymer that enables the required electric field gradient to be established. This polymer has good protein compatibility and allows the transport of small buffer ions while retaining large analytes such as proteins. With the use of an EFGF device, green fluorescent protein was concentrated 10 000-fold and the separation of a protein mixture was demonstrated. The development of these ionically conductive polymer-based devices represents a step toward making EFGF a useful analytical tool for proteomics investigations.

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mentioning

confidence: 99%

Electric Field Gradient Focusing of Proteins Based on Shaped Ionically Conductive Acrylic Polymer

Humble

Kelly²,

Woolley³

et al. 2004

Anal. Chem.

110

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Design types and performances of preparative polyacrylamide gel electrophoresis apparatus

Akaiwa¹

1982

Electrophoresis

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poly acrylamide gel electrophoresis apparatusThe designs of both gel and elution chambers in preparative electrophoresis apparatuses are key elements for their performance. The present paper describes four types of designs which were developed by the author to alleviate the shortcomings found in the preceding designs. Performance tests, using human hemoglobin (Hb), partially purified haptoglobin 2-1 type (Hp 2l), and bis-albumin-containing serum, show that insufficient dissipation of Joule heat and a heterogeneous electric field cause curvature and slant of the sample zones, and that only a chamber of optimal shape and size leads to minimal dilution, giving a good resolution. Moreover, notch, angle, and rim (Fig. 5), designed to support the gel, and tapering of the elution chamber to minimize its volume, are also important ministructures for the performance of the instrument. Unoptimal sets of these structures also cause concave sample bands and result in bad resolution and excessive dilution. These findings emphasize that in scaling up the apparatus, the structure must be designed on the basis of the detailed fundamental experiments from macrostructure to microstructure by using models of actual size. 0 Verlag Chemie GmbH, D-6940 Weinheim, 1982 0 1 73-0835/82/06 12-032 1 $2.50/0

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Electrophoresis of ribonucleic acid on a polyacrylamide gel which contains disulfide cross-linkages

Hansen

1976

Analytical Biochemistry

103

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A new membraneless device for preparative disc electrophoresis permitting concentrated elution

Cited by 6 publications

References 22 publications

Electric Field Gradient Focusing of Proteins Based on Shaped Ionically Conductive Acrylic Polymer

Electric Field Gradient Focusing of Proteins Based on Shaped Ionically Conductive Acrylic Polymer

Design types and performances of preparative polyacrylamide gel electrophoresis apparatus

Electrophoresis of ribonucleic acid on a polyacrylamide gel which contains disulfide cross-linkages

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