A new methanol assimilating yeast, Ogataea parapolymorpha, the ascosporic state of Candida parapolymorpha

Abstract

Ogataea parapolymorpha sp. n. (NRRL YB-1982, CBS 12304, type strain), the ascosporic state of Candida parapolymorpha, is described. The species appears homothallic, assimilates methanol as is typical of most Ogataea species and forms hat-shaped ascospores in asci that become deliquescent. O. parapolymorpha is closely related to Ogataea angusta and Ogataea polymorpha. The three species can be resolved from gene sequence analyses but are unresolved from fermentation and growth reactions that are typically used for yeast identification. On the basis of multiple isolates, O. angusta is known only from California, USA, in association with Drosophila and Aulacigaster flies, O. parapolymorpha is predominantly associated with insect frass from trees in the eastern USA but O. polymorpha has been isolated from various substrates in the USA, Brazil, Spain and Costa Rica.

Complete DNA Sequence of Kuraishia capsulata Illustrates Novel Genomic Features among Budding Yeasts (Saccharomycotina)

“…By comparison, O. polymorpha and O. parapolymorpha have kept an almost complete synteny (supplementary fig. S6, Supplementary Material online) in agreement with the fact that the two species are very close to each other (Kurtzman 2011). However, they both share only very short blocks of conserved synteny with D. bruxellensis , clearly indicating the broad evolutionary range covered by this group of yeasts.…”

“…This species, previously designated Hansenula capsulata (Wickerham 1952) or Pichia capsulata (Kurtzman 1984), is usually recovered from frass or tunnels of insect larvae underneath the bark of certain conifers (Kurtzman et al 2011). It belongs to a genus phylogenetically broadly related to the genera Citeromyces and Nakasawaea for which no genome data are presently available (Kurtzman et al 2011) and is even more distantly related to methanol-assimilating yeasts such as O. polymorpha and O. parapolymorpha (Suh and Zhou 2010; Kurtzman 2011). In K. capsulata , conjugation usually precedes ascus formation and ascospores (usually 1–2 per ascus) are hat-shaped.…”

Genomic insights into the evolution of industrial yeast speciesBrettanomyces bruxellensis

“…Brettanomyces naardinensis has not been isolated from fermentation environs, although its source (carbonated beverages) is still industrial (Kolfschoten & Yarrow, 1970), and the matrix in question is high in sugar with a low pH. They have been isolated from various sources, including spoilt orange juice, but are most commonly found in soil, tree exudates, insect frass and wood-ingesting insects (Suh & Zhou, 2010;Kurtzman, 2011;Morales et al, 2013). They have been isolated from various sources, including spoilt orange juice, but are most commonly found in soil, tree exudates, insect frass and wood-ingesting insects (Suh & Zhou, 2010;Kurtzman, 2011;Morales et al, 2013).…”

“…'Whole-genome'-based phylogenies(Curtin et al, 2012;Pi skur et al, 2012;Morales et al, 2013;Ravin et al, 2013) place B. bruxellensis in an intermediate evolutionary group with methylotropic species Komagetalla pastoris, Kuraishia capsulata and Ogataea angusta/O. The relative positions of other Brettanomyces species have been estimated based upon a separate multi-gene phylogeny(Kurtzman, 2011) (Right, purple branches). A multi-gene phylogeny(Kurtzman & Robnett, 2013) expands upon the relationship between these species and places K. pastoris outside of the B. bruxellensis containing clade (Right, red branches).…”

Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1

“…This strain is phylogenetically distinct from the majority of the Ogataea species complex [28] and is currently classified as Ogataea parapolymorpha DL-1 [23]. Such characteristics as resistance to heavy metals, oxidative stress, and thermotolerance also make the DL-1 strain an attractive host for various metabolic engineering purposes, for instance for development of novel ethanol producers [29].…”