A new method for absolute quantitation MRS metabolites

Barantin, Laurent; Pape, Alain Le; Akoka, Serge

doi:10.1002/mrm.1910380203

Cited by 144 publications

(148 citation statements)

References 15 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…All spectra were corrected for phase distortion and the baseline was manually corrected for each spectrum. To quantify this, the electronic reference to in vivo concentrations (ERETIC) signal (Barantin et al 1997) was used. It was generated using one of the frequency channels of the spectrometer and was sent to the carbon channel of the probe during the acquisition of the NMR signal (Akoka et al 1999).…”

Section: H-nmr Spectroscopymentioning

confidence: 99%

“…The NMR parameters of this ERETIC FID were under control. The ERETIC peak is provided as a quantitative reference signal that has the same area in all spectra (Barantin et al 1997). Spectral 1 H assignments were made based on the literature values of chemical shifts in various media and biofluids (Nicholson et al 1995, Maillet et al 1998.…”

Section: H-nmr Spectroscopymentioning

confidence: 99%

See 1 more Smart Citation

Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality

Bertoldo¹,

Nadal-Desbarats²,

Gérard³

et al. 2013

Reproduction

View full text Add to dashboard Cite

The microenvironment of the developing follicle is critical to the acquisition of oocyte developmental competence, which is influenced by several factors including follicle size and season. The aim of this study was to characterise the metabolomic signatures of porcine follicular fluid (FF) collected from good and poor follicular environments, using high-resolution proton nuclear magnetic resonance ( 1 H-NMR) spectroscopy. Sow ovaries were collected at slaughter, 4 days after weaning, in summer and winter. The contents of small (3-4 mm) and large (5-8 mm) diameter follicles were aspirated and pooled separately for each ovary pair. Groups classified as summer-small (nZ8), summer-large (nZ15), winter-small (nZ9) and winter-large (nZ15) were analysed by 1 H-NMR spectroscopy.The concentrations of 11 metabolites differed due to follicle size alone (P!0.05), including glucose, lactate, hypoxanthine and five amino acids. The concentrations of all these metabolites, except for glucose, were lower in large FF compared with small FF. Significant interaction effects of follicle size and season were found for the concentrations of glutamate, glycine, N-acetyl groups and uridine. Succinate was the only metabolite that differed in concentration due to season alone (P!0.05). The FF levels of progesterone, androstenedione and oestradiol were correlated with the concentrations of most of the metabolites examined. The results indicate that there is a distinct shift in follicular glucose metabolism as follicles increase in diameter and suggest that follicular cells may be more vulnerable to oxidative stress during the summer months. Our findings demonstrate the power of 1 H-NMR spectroscopy to expand our understanding of the dynamic and complex microenvironment of the developing follicle.

show abstract

Section: H-nmr Spectroscopymentioning

confidence: 99%

Section: H-nmr Spectroscopymentioning

confidence: 99%

Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality

Bertoldo¹,

Nadal-Desbarats²,

Gérard³

et al. 2013

Reproduction

View full text Add to dashboard Cite

show abstract

“…Concentration measurements of metabolites of interest in all samples were performed using the recently implemented Bruker 'Digital ERETIC' (digital electronic reference to access in vivo concentrations) tool package that is based on the ERETIC 48 and PULCON 49 (pulse length-based concentration determination) methods. Briefly, this method uses a reference sample of known concentration to first calibrate a digital ERETIC signal that is then added at a chosen frequency in the reference spectrum.…”

Section: Nmr Quantificationmentioning

confidence: 99%

Serum metabolome analysis by 1H-NMR reveals differences between chronic lymphocytic leukaemia molecular subgroups

et al. 2010

View full text Add to dashboard Cite

Chronic lymphocytic leukaemia (CLL) is a heterogeneous disease exhibiting variable clinical course and survival rates. Mutational status of the immunoglobulin heavy chain variable regions (IGHVs) of CLL cells offers useful prognostic information for high-risk patients, but time and economical costs originally prevented it from being routinely used in a clinical setting. Instead, alternative markers of IGHV status, such as zeta-associated protein (ZAP70) or messenger RNA levels are often used. We report a 1 H-NMR-based metabolomics approach to examine serum metabolic profiles of early stage, untreated CLL patients (Binet stage A) classified on the basis of IGHV mutational status or ZAP70. Metabolic profiles of CLL patients (n ¼ 29) exhibited higher concentrations of pyruvate and glutamate and decreased concentrations of isoleucine compared with controls (n ¼ 9). Differences in metabolic profiles between unmutated (UM-IGHV; n ¼ 10) and mutated IGHV (M-IGHV; n ¼ 19) patients were determined using partial least square discriminatory analysis (PLS-DA; R 2 ¼ 0.74, Q 2 ¼ 0.36). The UM-IGHV patients had elevated levels of cholesterol, lactate, uridine and fumarate, and decreased levels of pyridoxine, glycerol, 3-hydroxybutyrate and methionine concentrations. The PLS-DA models derived from ZAP70 classifications showed comparatively poor goodness-of-fit values, suggesting that IGHV mutational status correlates better with diseaserelated metabolic profiles. Our results highlight the usefulness of 1 H-NMR-based metabolomics as a potential non-invasive prognostic tool for identifying CLL disease-state biomarkers.

show abstract

“…Since analyte quantification is an important aspect of NMR hyphenation, use of the ERETIC method (Electronic REference To access In vivo Concentrations), originally introduced for internal calibration of in vivo NMR spectra [88], is of interest. The method, which relies on the generation of a synthetic reference signal with defined frequency and amplitude, was subsequently adapted for solution studies [89], [90], [91], and can be used as a means of quantification of HPLC-NMR data.…”

Section: Other New Trends In Nmr Hyphenationmentioning

confidence: 99%

Untitled

2005

Planta Med

View full text Add to dashboard Cite

IntroductionIn the first part of this review [1], the use of direct NMR hyphenation in natural products research has been discussed. The direct HPLC-NMR methods were defined as methods in which the NMR data acquisition is performed on the HPLC column eluate [1]. This implies either a direct connection between the NMR spectrometer and the HPLC apparatus, or use of storage capillaries in which the HPLC eluate is temporarily stored prior to acquisition of NMR data.Many useful results related to natural products have been obtained using direct HPLC-NMR methods [1] [30]. However, the direct combination of state-of-the-art separation technology with the most powerful and versatile tool for structure elucidation of organic molecules in solution suffers from various practical problems. In short, these practical limitations are due to somewhat contradictory requirements of HPLC and NMR with respect to solvents and time-scale of the experiment, and thus compromises are necessary in order to hyphenate these two techniques. For example, 1 H-NMR spectra are preferably acquired in deuterated solvents, but use of deuterated solvents for HPLC is expensive and the selection is limited. Some useful HPLC eluents, such as buffers with high ionic strengths, are directly undesirable in direct HPLC-NMR hyphenation. Gradient elution of HPLC columns, required in most separations, implies constantly changing magnetic susceptibility and field homogeneity in the NMR flowprobe, not to mention variations of analyte chemical shifts with solvent composition. Hyphenated NMR Methods in Natural Products AbstractThis review describes the principles and performance of a novel and highly promising hyphenated technique, HPLC-SPE-NMR, which is based on post-column analyte trapping by solid-phase extraction. The analytes are subsequently eluted from the SPE cartridges using deuterated solvents. This indirect HPLC-NMR hyphenation offers numerous advantages compared to direct HPLC-NMR methods. Multiple trapping leads to a dramatic increase of analyte amounts available for NMR, enabling acquisition of high-quality 2D NMR data within a short time. Other new developments, including combination of solenoidal coil capillary flow-probes with microflow HPLC, are also discussed. Fast extract dereplication using these techniques enables focusing of isolation efforts on truly novel and promising natural products, based on precise structural data obtained with crude extracts or fractions.

show abstract

A new method for absolute quantitation MRS metabolites

Cited by 144 publications

References 15 publications

Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality

Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality

Serum metabolome analysis by 1H-NMR reveals differences between chronic lymphocytic leukaemia molecular subgroups

Untitled

Contact Info

Product

Resources

About