A new method for controlling rice blast (Magnaporthe grisea) using effective microbes as biocontrol agents

Abstract

Rice blast caused by Magnaporthe grisea is a noteworthy ailment influencing rice developed zone. Use of biocontrol agents (BCA) seen as an option in contrast to the utilization of synthetic fungicide since biocontrol is an environmentally friendly plant disease controlling approach. The goal of this exploration is planning to screen natural control agents for controlling M. grisea, and considering biocontrol is a natural un-hurtful plant disease controlling original thought. 639 bacterial isolates were screened from various parts of rice tissues, of which potential biocontrol scores were distinguished by their capacities in antagonism inhibition and secreting extracellular hydrolytic protein. Biocontrol tests against M. grisea were done on 30 bacterial isolates with potential biocontrol limit after pre-choice through ARDRA and novel investigation on strains with high assessment scores. 9 BCAs with clear biocontrol activity (over 50%) in greenhouse tests were obtained. Bacillus cereus H-L out 6 and Bacillus subtilis D-S in 61 performed well in greenhouse tests. In general, correlation coefficient is 0.80 between analysis points of 35 tried BCA strains and correlation coefficient between antagonism test and biocontrol efficacy demonstrate 0.76 against M.grisea. Biocontrol efficacies results in greenhouse tests indicated positive correlation with analysis points, suggesting that the BCAs screening and method developed is adaptable for screening and controlling M.grisea.