A new method for in vitro detection of microbially produced mitochondrial toxins

Hoornstra, Douwe; Andersson, Maria A.; Mikkola, Raimo; Salkinoja‐Salonen, Mirja

doi:10.1016/s0887-2333(03)00097-3

Cited by 74 publications

(82 citation statements)

References 19 publications

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“…The boar sperm motility inhibition assay, including all the controls for aging and vehicle-exposed cells and for the repeatability of the assay, was executed as described previously (2). The change in the mitochondrial potential (⌬⌿ m ) and the change in the plasma membrane potential (⌬⌿ p ) in boar spermatozoa were recorded by use of the fluorogenic dye JC-1 (5,5=,6,6=-tetrachloro-1,1=,3,3=-tetraethylbenzimidazolylcarbocyanine iodide) as described previously by Hoornstra et al (27). For the estimation of the 100% effective concentration (EC 100 ), 10 microscopic fields at ϫ10 and ϫ40 magnifications were inspected.…”

Section: Or 50 G Of Nacl Litermentioning

confidence: 99%

“…Acidification was also visible as a change of the indicator color in RPMI medium from pink to yellow. Mitochondrial depolarization was read by a microscope after 26 h of exposure to the toxicants from the monolayers double stained with the membrane-potential-responsive fluorogenic dye JC-1 and with propidium iodide to assess the relaxing of the plasma membrane permeability barrier, similarly to what was reported previously for sperm cells (27).…”

Section: Or 50 G Of Nacl Litermentioning

confidence: 99%

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Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Rasimus

Mikkola

Andersson

et al. 2012

Appl Environ Microbiol

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Section: Or 50 G Of Nacl Litermentioning

confidence: 99%

Section: Or 50 G Of Nacl Litermentioning

confidence: 99%

Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Rasimus

Mikkola

Andersson

et al. 2012

Appl Environ Microbiol

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“…Both toxins form complexes with alkali metal ions (22), but cereulide has been suggested to have a higher toxicity than valinomycin due to its higher affinity for potassium (23). The described effect of cereulide on mitochondria is used in several assays to detect the presence of cereulide in food products, like the sperm motility inhibition assay (2,11,19) and the HEp-2 (human carcinoma of the larynx) cell vacuolation assay (6,8,14).…”

mentioning

confidence: 99%

Quantification of the Emetic Toxin Cereulide in Food Products by Liquid Chromatography-Mass Spectrometry Using Synthetic Cereulide as a Standard

Biesta-Peters

Reij

Blaauw³

et al. 2010

Appl Environ Microbiol

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Bacillus cereus produces the emetic toxin cereulide, a cyclic dodecadepsipeptide that can act as a K ؉ ionophore, dissipating the transmembrane potential in mitochondria of eukaryotic cells. Because pure cereulide has not been commercially available, cereulide content in food samples has been expressed in valinomycin equivalents, a highly similar cyclic potassium ionophore that is commercially available. This research tested the biological activity of synthetic cereulide and validated its use as a standard in the quantification of cereulide contents in food samples. The synthesis route consists of 10 steps that result in a high yield of synthetic cereulide that showed biological activity in the HEp-2 cell assay and the boar sperm motility assay. The activity is different in both methods, which may be attributed to differences in K ؉ content of the test media used. Using cereulide or valinomycin as a standard to quantify cereulide based on liquid chromatography-mass spectrometry (LC-MS), the concentration determined with cereulide as a standard was on average 89.9% of the concentration determined using valinomycin as a standard. The recovery experiments using cereulide-spiked food products and acetonitrile as extraction solute showed that the LC-MS method with cereulide as a standard is a reliable and accurate method to quantify cereulide in food, because the recovery rate was close to 100% over a wide concentration range.

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“…To test the toxic effect of cadmium on spermatozoa we applied the approach of Hoornstra et al (2003) with modifications. Cadmium chloride (anhydrous 99.99%, 11860, Alfa Aesar) stock solution was prepared at the concentration of 1 mg/mL in distilled water.…”

Section: Methodsmentioning

confidence: 99%

Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium — Short communication

Nagy

Kakasi

Bercsényi

2016

Acta Veterinaria Hungarica

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The aim of the present pilot study was to apply a flow cytometric assay, the so-called OxyDNA test, to determine the level of oxidative DNA damage in fish spermatozoa exposed to different concentrations (0.01-10,000 mg/L) of cadmium. Milt was collected from three randomly selected Prussian carp (Carassius auratus gibelio) males. Oxidative DNA damage was assessed with the OxyDNA kit and using flow cytometry. The ratio of OxyDNA-positive events increased significantly at higher cadmium concentrations. The results indicate that direct contact of fish spermatozoa with cadmium-polluted water initiates genotoxic damage.

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A new method for in vitro detection of microbially produced mitochondrial toxins

Cited by 74 publications

References 19 publications

Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Quantification of the Emetic Toxin Cereulide in Food Products by Liquid Chromatography-Mass Spectrometry Using Synthetic Cereulide as a Standard

Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium — Short communication

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