A new method for measuring surface acoustic wave speeds by acoustic microscopes and its application in characterizing laterally inhomogeneous materials

Ghosh, T.; Maslov, Konstantin; Kundu, Tribikram

doi:10.1016/s0041-624x(97)00012-7

Cited by 7 publications

(3 citation statements)

References 35 publications

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“…The method of investigating cells on silicon rubber was introduced by Harris (Harris, 1982;Harris et al, 1980). V(f) curves have recently been used by materials scientists for characterizing horizontally and vertically layered materials (Nagy and Adler, 1990;Ghosh et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Cell Property Determination from the Acoustic Microscope Generated Voltage Versus Frequency Curves

Kundu

Bereiter‐Hahn

Karl

2000

Biophysical Journal

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Among the methods for the determination of mechanical properties of living cells acoustic microscopy provides some extraordinary advantages. It is relatively fast, of excellent spatial resolution and of minimal invasiveness. Sound velocity is a measure of the stiffness or Young's modulus of the cell. Attenuation of cytoplasm is a measure of supramolecular interactions. These parameters are of crucial interest for studies of cell motility, volume regulations and to establish the functional role of the various elements of the cytoskeleton. Using a phase and amplitude sensitive modulation of a scanning acoustic microscope (Hillman et al., 1994, J. Alloys Compounds. 211/212:625-627) longitudinal wave speed, attenuation and thickness profile of a biological cell are obtained from the voltage versus frequency or V(f) curves. A series of pictures, for instance in the frequency range 980-1100 MHz with an increment of 20 MHz, allows the experimental generation of V(f) curves for each pixel while keeping the lens-specimen distance unchanged. Both amplitude and phase values of the V(f) curves are used for obtaining the cell properties and the cell thickness profile. The theoretical analysis shows that the thin liquid layer, between the cell and the substrate, has a strong influence on the reflection coefficient and should not be ignored during the analysis. Cell properties, cell profile and the thickness of the thin liquid layer are obtained from the V(f) curves by the simplex inversion algorithm. The main advantages of this new method are that imaging can be done near the focal plane, therefore an optimal signal to noise ratio is achieved, no interference with Rayleigh waves occurs, and the method requires only an approximate estimate of the material properties of the solid substratum where the cells are growing on.

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Section: Introductionmentioning

confidence: 99%

Cell Property Determination from the Acoustic Microscope Generated Voltage Versus Frequency Curves

Kundu

Bereiter‐Hahn

Karl

2000

Biophysical Journal

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“…Bogy and Gracewski obtained an analytical expression of the reflection coefficient for a one-layer system, which can be used for the simulation of V(ω, z) [54 -56]. In SAM, the curve V(ω, z) is often called V(f) [37,57,58].…”

Section: Theory Of Time-resolved Acoustic Microscopymentioning

confidence: 99%

High-Frequency Time-Resolved Scanning Acoustic Microscopy for Biomedical Applications

Anastasiadis¹,

Zinin²

2018

TONIJ

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High-frequency focused ultrasound has emerged as a powerful modality for both biomedical imaging and elastography. It is gaining more attention due to its capability to outperform many other imaging modalities at a submicron resolution. Besides imaging, high-frequency ultrasound or acoustic biomicroscopy has been used in a wide range of applications to assess the elastic and mechanical properties at the tissue and single cell level. The interest in acoustic microscopy stems from the awareness of the relationship between biomechanical and the underlying biochemical processes in cells and the vast impact these interactions have on the onset and progression of disease. Furthermore, ultrasound biomicroscopy is characterized by its non-invasive and non-destructive approach. This, in turn, allows for spatiotemporal studies of dynamic processes without the employment of histochemistry that can compromise the integrity of the samples. Numerous techniques have been developed in the field of acoustic microscopy. This review paper discusses high-frequency ultrasound theory and applications for both imaging and elastography.

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“…The dispersion of the Rayleigh wave can be used to extract elastic mechanical parameters like the Young´s modulus, because the Rayleigh wave velocity depends on the density as well as the elastic constants. In this sense, the determination of the Rayleigh wave velocities is of high interest for the characterization of materials-especially of coatings-and was studied via LUS in, e.g., [3][4][5][6] and via SAM in, e.g., [8,[16][17][18][19]. In the past, I(z) measurements have been investigated analytically via ray theory [7,20,21] and via wave theory approaches [7,22].…”

Section: Introductionmentioning

confidence: 99%

Simulation of Acoustic Wave Propagation in Aluminium Coatings for Material Characterization

et al. 2017

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Aluminium coatings and their characterization are of great interest in many fields of application, ranging from aircraft industries to microelectronics. Here, we present the simulation of acoustic wave propagation in aluminium coatings via the elastodynamic finite integration technique (EFIT) in comparison to experimental results. The simulations of intensity (I)-defocus (z) curves, obtained by scanning acoustic microscopy (SAM), were first carried out on an aluminium bulk sample, and secondly on a 1 µm aluminium coating deposited on a silicon substrate. The I(z) curves were used to determine the Rayleigh wave velocity of the aluminium bulk sample and the aluminium coating. The results of the simulations with respect to the Rayleigh velocity were corroborated by non-destructive SAM measurements and laser ultrasonic measurements (LUS).

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A new method for measuring surface acoustic wave speeds by acoustic microscopes and its application in characterizing laterally inhomogeneous materials

Cited by 7 publications

References 35 publications

Cell Property Determination from the Acoustic Microscope Generated Voltage Versus Frequency Curves

Cell Property Determination from the Acoustic Microscope Generated Voltage Versus Frequency Curves

High-Frequency Time-Resolved Scanning Acoustic Microscopy for Biomedical Applications

Simulation of Acoustic Wave Propagation in Aluminium Coatings for Material Characterization

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