A new method for meniscus repair using type I collagen scaffold and infrapatellar fat pad

Oda, Shunsuke; Otsuki, Shuhei; Kurokawa, Yoshitaka; Hoshiyama, Yoshiaki; Nakajima, Mikio; Neo, Masashi

doi:10.1177/0885328215568984

Cited by 34 publications

(35 citation statements)

References 37 publications

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“…The increased expression of Ki67 marker in UCM treated cells compared to control, clearly indicates that supplementation of UCM to DMEM significantly influence meniscal cell proliferation. Similar increase in Ki67 positive expression was reported in meniscus cells grown in collagen type I scaffold along with infrapatellar fat pad (Oda et al 2015). CD34 marker expression was found to be absent in both the control and UCM supplemented meniscal cells indicating that the UCM does not induce any differentiation during the proliferation.…”

Section: Discussionsupporting

confidence: 79%

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study

et al. 2015

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The present study investigates the impact of biomolecules (biotin, glucose, chondroitin sulphate, proline) as supplement, (individual and in combination) on primary human meniscus cell proliferation. Primary human meniscus cells isolated from patients undergoing meniscectomy were maintained in Dulbecco's Modified Eagle's Medium (DMEM). The isolated cells were treated with above mentioned biomolecules as individual (0-100 lg/ml) and in combinations, as a supplement to DMEM. Based on the individual biomolecule study, a unique combination of biomolecules (UCM) was finalized using one way ANOVA analysis. With the addition of UCM as supplement to DMEM, meniscal cells reached 100 % confluency within 4 days in 60 mm culture plate; whereas the cells in medium devoid of UCM, required 36 days for reaching confluency. The impact of UCM on cell viability, doubling time, histology, gene expression, biomarkers expression, extra cellular matrix synthesis, meniscus cell proliferation with respect to passages and donor's age were investigated. The gene expression studies for E-cadherin and peroxisome proliferator-activated receptor (PPARD) using RT-qPCR and immunohistochemical analysis for Ki67, CD34 and Vimentin confirmed that UCM has significant impact on cell proliferation. The extracellular collagen and glycosaminoglycan secretion in cells supplemented with UCM were found to increase by 31 and 37 fold respectively, when compared to control on the 4th day. The cell doubling time was reduced significantly when supplemented with UCM. The addition of UCM showed positive influence on different passages and age groups. Hence, this optimized UCM can be used as an effective supplement for meniscal tissue engineering.

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Section: Discussionsupporting

confidence: 79%

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study

et al. 2015

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“…In this study, a full thickness cylindrical meniscal defect was used to evaluate scaffold performance. This defect type has been previously described in small animal models [11,26] and is optimal to ensure that the scaffold remains in situ during the experimental period. Previous studies using partial meniscectomy models have reported scaffold loss as a significant impact on the analysis of results [4] .…”

Section: Discussionmentioning

confidence: 99%

A novel biphasic scaffold supports meniscal tissue repair in ex vivo and in vivo models

2018

Musculoskelet Regen

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“…Radial-longitudinal tears [173] Electro spun type I collagen scaffolds and vascular/avascular region meniscal cells Avascular meniscal tears [174] Radio opaque electro spun scaffold Meniscal regrowth [176] Wrapping of meniscal defects with collagen membrane and injection of MSCs Tears in avascular zone [156] Aligned electro spun nano fibrous scaffold Radial tear [178] Collagen gel scaffold or HA hydrogel delivery of meniscal, synovial and adipose cells Bucket handle tear [177] Type I collagen scaffold/ infrapatellar fat pad Anterior 2 mm round holes [179] Chondrocyte + PLGA mesh scaffold + PRP Chondrocytes evaluated [180] Meniscal cells in fibre reinforced collagen-GAG scaffold + PRP Gene profiling study [168]…”

Section: Scaffolds Lesion and Study Type Referencesmentioning

confidence: 99%

“…Collagen gel scaffolds containing meniscal, synovial and adipose stem cells have been employed for meniscal repair [177] or in electrospun nanofibrous scaffolds [178]. The use of a type I collagen scaffold and infrapatellar fat pad for meniscal repair has been evaluated in rabbits [179]. PLGA mesh and fibre reinforced collagen-GAG scaffolds seeded with chondrocytes [180] or meniscal cells [168] supplemented with PRP have been evaluated for meniscal repair.…”

Section: Bioscaffolds Bioactive Substances and Bioadhesives And Menimentioning

confidence: 99%

Novel Approaches in Meniscal Repair Utilizing Mesenchymal Stem Cells, New Generation Bioscaffolds and Biological Adhesives as Cell Delivery Vehicles

Melrose¹

2019

Meniscus of the Knee - Function, Pathology and Management

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Mesenchymal stem cells (MSCs) have been widely applied in the repair of the knee-joint menisci which have a limited ability to undergo spontaneous repair. The menisci stabilise the knee-joint and are weight-bearing structures subjected to considerable tensional and compressive forces during flexion-extension and torsional loading of the knee. Traumatic loading of the knee-joint menisci can generate a number of lesions in the inner avascular meniscal regions. These have a limited capability of intrinsic repair and predispose the underlying articular cartilages to premature osteoarthritis. A number of strategies have therefore been developed for meniscal repair employing MSCs, bioscaffolds, hydrogels, biological glue cell delivery systems and agents which promote cell proliferation/matrix synthesis. Meniscal implants have also been developed in combination with the above procedures. It is important that meniscal defects be repaired not only to maintain kneejoint stability but also to prevent further degenerative changes in other knee joint tissues. Degenerative menisci contribute degradative proteinases and inflammatory mediators to the total synovial degradative proteinase pool. Partial or total surgical removal of the menisci is not a solution since this leads to premature osteoarthritis. Meniscal integrity needs to be maintained or repair strategies implemented in a timely manner to maintain knee joint function.

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A new method for meniscus repair using type I collagen scaffold and infrapatellar fat pad

Abstract: This new method for meniscal repair by combinative transplantation with type I collagen scaffold and infrapatellar fat pad showed meniscal regeneration and potential for suppressing inflammation.

Cited by 34 publications

References 37 publications

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study

A novel biphasic scaffold supports meniscal tissue repair in ex vivo and in vivo models

Novel Approaches in Meniscal Repair Utilizing Mesenchymal Stem Cells, New Generation Bioscaffolds and Biological Adhesives as Cell Delivery Vehicles

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