A new method for molecular sex identification in the emu (
            <i>Dromaius novaehollandiae</i>
            )

Jia, Xuebo; Lü, Jing; Tang, Xiujun; Fan, Yanfeng; YuShi, Gao

doi:10.1080/00071668.2022.2163876

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Sex identification using the CHD gene was first proposed by Griffiths ( Griffiths, 1991 ). At present, this method has achieved early sex identification in chickens ( Ayers et al, 2015 ), ducks ( Li et al, 2015 ), pigeons ( Abou Elez et al, 2023 ), partridges ( Jia et al, 2023 ), ostriches ( Huang C and Huang M, 2008 ), and other species. There are two homologous copies of the CHD gene, of which CHD1-W is linked to the W chromosome and CHD1-Z is linked to the Z chromosome ( Lai et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

The development rule of feathers and application of hair root tissue in sex identification of Yangzhou geese

Qi,

Xu,

Liu

et al. 2024

Poultry Science

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Section: Discussionmentioning

confidence: 99%

The development rule of feathers and application of hair root tissue in sex identification of Yangzhou geese

Qi,

Xu,

Liu

et al. 2024

Poultry Science

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New primers for sex identification in the Chukar partridges ( Alectoris chukar )

Jia,

Chen,

Tang

et al. 2024

British Poultry Science

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Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfishOplegnathus fasciatus

Xiao,

Liu

et al. 2024

GigaScience

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Background The use of sex-specific molecular markers has become a prominent method in enhancing fish production and economic value, as well as providing a foundation for understanding the complex molecular mechanisms involved in fish sex determination. Over the past decades, research on male and female sex identification has predominantly employed molecular biology methodologies such as restriction fragment length polymorphism, random amplification of polymorphic DNA, simple sequence repeat, and amplified fragment length polymorphism. The emergence of high-throughput sequencing technologies, particularly Illumina, has led to the utilization of single nucleotide polymorphism and insertion/deletion variants as significant molecular markers for investigating sex identification in fish. The advancement of sex-controlled breeding encounters numerous challenges, including the inefficiency of current methods, intricate experimental protocols, high costs of development, elevated rates of false positives, marker instability, and cumbersome field-testing procedures. Nevertheless, the emergence and swift progress of PacBio high-throughput sequencing technology, characterized by its long-read output capabilities, offers novel opportunities to overcome these obstacles. Findings Utilizing male/female assembled genome information in conjunction with short-read sequencing data survey and long-read PacBio sequencing data, a catalog of large-segment (>100 bp) insertion/deletion genetic variants was generated through a genome-wide variant site-scanning approach with bidirectional comparisons. The sequence tagging sites were ranked based on the long-read depth of the insertion/deletion site, with markers exhibiting lower long-read depth being considered more effective for large-segment deletion variants. Subsequently, a catalog of bulk primers and simulated PCR for the male/female variant loci was developed, incorporating primer design for the target region and electronic PCR (e-PCR) technology. The Japanese parrotfish (Oplegnathus fasciatus), belonging to the Oplegnathidae family within the Centrarchiformes order, holds significant economic value as a rocky reef fish indigenous to East Asia. The criteria for rapid identification of male and female differences in Japanese parrotfish were established through agarose gel electrophoresis, which revealed 2 amplified bands for males and 1 amplified band for females. A high-throughput identification catalog of sex-specific markers was then constructed using this method, resulting in the identification of 3,639 (2,786 INS/853 DEL, ♀ as reference) and 3,672 (2,876 INS/833 DEL, ♂ as reference) markers in conjunction with 1,021 and 894 high-quality genetic sex identification markers, respectively. Sixteen differential loci were randomly chosen from the catalog for validation, with 11 of them meeting the criteria for male/female distinctions. The implementation of cost-effective and efficient technological processes would facilitate the rapid advancement of genetic breeding through expediting the high-throughput development of sex genetic markers for various species. Conclusions Our study utilized assembled genome information from male and female individuals obtained from PacBio, in addition to data from short-read sequencing data survey and long-read PacBio sequencing data. We extensively employed genome-wide variant site scanning and identification, high-throughput primer design of target regions, and e-PCR batch amplification, along with statistical analysis and ranking of the long-read depth of the variant sites. Through this integrated approach, we successfully compiled a catalog of large insertion/deletion sites (>100 bp) in both male and female Japanese parrotfish.

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A new method for molecular sex identification in the emu ( Dromaius novaehollandiae )

Cited by 3 publications

References 15 publications

The development rule of feathers and application of hair root tissue in sex identification of Yangzhou geese

The development rule of feathers and application of hair root tissue in sex identification of Yangzhou geese

New primers for sex identification in the Chukar partridges ( Alectoris chukar )

Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfishOplegnathus fasciatus

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