A new method for mutation inducing in rice by using DC electrophoresis bath and its mutagenic effects

Abstract

Mutation breeding is a significant means of increasing breeding efficiency and accelerating breeding process. In present study, we explored a new method for mutations inducing in rice (Oryza sativa L.) by using direct current electrophoresis bath (DCEB). The results showed that 20 mM NaCl solution is the optimal buffer, and the mortality of rice seeds followed an upward trend with increasing voltage and processing time of DCEB. By exploring the mutagenic effects of γ-irradiation and DCEB on seed vigor and physiological damages, we found that the physiological damages induced by DCEB on seed vigor were significant compared with that by γ-irradiation. We screened two mutants with low filled grain percentage and one mutant with abnormal hull from the M2 generations. These three mutants were confirmed to be authentic mutants based on 48 SSR markers followed by the protocol NY/T 1433–2014. Whole-genome resequencing detected a total of 503 and 537 polymorphisms in the two mutants, respectively, and the DCEB mutagenesis induced mainly InDel variants, while the exon region of mutant genes occupied a large proportion, especially the SNP variants, which occupied about 20% of the mutation sites in the exon region.

Exploring the general public’s and experts’ risk and benefit perceptions of cultured meat in Singapore: A mental models approach

“…For instance, a study by Byram et al [ 28 ] examining perceptions of local complications related to breast implants found that women’s beliefs about their personal risk of complications often diverged from those of medical specialists (i.e., experts) when comparing their mental models. Hagemann and Scholderer [ 26 ] also conducted a study comparing the mental models of experts and laypersons regarding mutation-bred rice, a type of rice varieties that have been developed through the process of induced mutation that involves the deliberate exposure of rice seeds or plant tissues to radiation or chemicals to induce genetic changes or mutations [ 32 ]. They found that experts were more focused on risks assessable within legal frameworks, whereas consumers expressed other concerns, such as environmental risks that fell outside these frameworks.…”

Phenotypic and genetic characterization of an Avena sativa L. germplasm collection of diverse origin: implications for food-oat breeding in Chile

“…In oats, TILLING allowed to induce variation in genes encoding for enzymes involved in the pathways of lignin and beta-glucan biosynthesis (Chawade et al, 2010). Recently, mutagenesis by direct current electrophoresis bath (DCEB) was investigated in rice (Zou et al, 2023). Mutation by exposing seeds or plants to cosmic radiation in outer space, or space-breeding, has allowed the release of at least 66 varieties in China (Liu et al, 2009;Mohanta et al, 2021).…”