A new method for quantification of melamine in milk by absorption diode-array thin-layer chromatography

Broszat, Melanie; Brämer, Regina; Spangenberg, Bernd

doi:10.1556/jpc.21.2008.6.14

Cited by 24 publications

(17 citation statements)

References 3 publications

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“…Currently, various methods-including thin-layer chromatography [6], gas chromatography (GC) [7,8], high-performance liquid chromatography [9][10][11][12], enzyme-linked immunosorbent assay technology [13], matrix-assisted laser desorption/ ionization mass spectrometry (MALDI-MS) [14], and surface desorption atmospheric pressure chemical ionization mass spectrometry [15]-have been used to detect melamine in food. However, these methods usually suffer from drawbacks, such as the need for a complicated sample preparation process, clean-up steps, and a time-consuming determination process, so they cannot fulfill the requirement for fast in situ detection.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of melamine with 4-mercaptopyridine-modified gold nanoparticles by surface-enhanced Raman scattering

et al. 2011

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A surface-enhanced Raman scattering (SERS) strategy based on 4-mercaptopyridine (MPY)-modified gold nanoparticles (AuNPs) was developed for the rapid and sensitive detection of melamine in milk powder. The SERS measurement of melamine strongly relied on the "hotspot" effect, in which AuNPs immediately aggregated upon the addition of melamine, leading to significantly enhanced Raman intensity of the reporter molecule MPY and a color change for the solution from red to blue-gray. The limit of detection based on a signal to noise of 3 (S/N=3) was found to be as low as 0.1 ppb of melamine, with an excellent linearity of 0.5-100 ppb, demonstrating a higher sensitivity and a wider quantitation range than direct SERS sensing methods based on enhanced substrate. An impressive specificity for melamine detection over various common metal ions and excipients in dairy products, even at concentrations of 100-fold higher than melamine, was achieved. Good recoveries of 88.5% and 111.7% were obtained from milk samples spiked to 20 and 100 ppb levels, respectively. The proposed method is potentially applicable for the rapid in situ determination of melamine in complex matrices.

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Section: Introductionmentioning

confidence: 99%

Rapid detection of melamine with 4-mercaptopyridine-modified gold nanoparticles by surface-enhanced Raman scattering

et al. 2011

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“…Loaded plates were developed to a distance of 80 mm using mobile phase (iso-propanol/ dichloromethane/water, 5:2.5:3, v/v/v) in Camag twin-trough plate development chamber (Broszat et al 2008). It was lined with a filter paper and pre-saturated for 5 min with mobile phase (10 mL) before use.…”

Section: Chromatographic Analysismentioning

confidence: 99%

“…Analytical methods such as gas chromatography (Toth and Bardalaye 1987), liquid chromatography/mass spectrometry (Fligenzi et al 2007), diode array thin layer chromatography (Broszat et al 2008), capillary electrophoresis (Garber 2008), and enzymelinked immunosorbent assay (Shan-Shan et al 1999) are officially recognized reference methods for the detection of melamine contamination in food stuffs. However, in order to develop a faster, low-cost, and routine method for economic adulteration of melamine in milk particularly for the laboratories not having expensive LC-MS infrastructure, high performance thin layer chromatography (HPTLC) is proved to be a boon.…”

Section: Introductionmentioning

confidence: 99%

HPTLC-MS analysis of melamine in milk: standardization and validation

Rani

Medhe

Srivastava

2014

Dairy Sci. & Technol.

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Melamine containing large amount of nitrogen may be deliberately added to milk in order to increase the protein content for economic adulteration in food stuffs. High performance thin layer chromatography-mass spectrometry (HPTLC-MS)-based method of quantification of melamine has been developed using Silica gel 60 F 254s HPTLC plates, with optimized mobile phase iso-propanol/dichloromethane/water, 5:2.5:3, v/v/v in a twin trough chamber saturated (5 min) at pH 6.8. Validation was carried out by testing its specificity, linearity (1-50 μg), accuracy (95.908-98.369%), inter-and intraday precision (1.578 and 1.670%), respectively, limits of detection (2.688 ng) and quantification (8.146 ng). HPTLC in combination with MS has been found suitable for routine analysis of melamine added as adulterant to market-purchased milk samples.

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“…2009; Zhou et al 2009a;Nkem Ihunegbo et al 2010;Desmarchelier et al 2009;Heller and Nochetto 2008;Kim et al 2010), gas chromatography ), thin-layer chromatography (Broszat et al 2008), ultra-performance liquid chromatography (Xia et al 2009) and some others, have been developed to monitor MEL in milk products, eggs as well as other foods and components.…”

Section: Introductionmentioning

confidence: 99%

Rapid and High-Throughput Determination of Melamine in Milk Products and Eggs by Full Automatic On-line Polymer Monolith Microextraction Coupled to High-Performance Liquid Chromatography

2010

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A rapid and high-throughput method for monitoring melamine (MEL) in milk products and eggs was presented. This method was based on a full automatic platform that was composed of on-line polymer monolith microextraction and high-performance liquid chromatography with ultraviolet detection. A poly(2-acrylamido-2-methyl-1-propanesulfonic acid-co-ethylene dimethacrylate) monolith was selected as the sorbent for purification and enrichment of MEL in milk products and eggs. A novel mixed-mode chromatographic column packed with octyl and sulfonic acid co-bonded silica was employed for quantitative determination of MEL in real samples. Several factors affecting extraction performance were investigated. Under the optimal conditions, the recoveries of MEL in milk products and eggs spiked at three levels of 0.5, 5.0, and 20.0 mg/Kg, ranged from 87.0% to 95.5%, with RSDs less than 6.4%. The limits of detection were 0.024 and 0.018 mg/Kg for MEL in the milk products and eggs, respectively.

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A new method for quantification of melamine in milk by absorption diode-array thin-layer chromatography

Cited by 24 publications

References 3 publications

Rapid detection of melamine with 4-mercaptopyridine-modified gold nanoparticles by surface-enhanced Raman scattering

Rapid detection of melamine with 4-mercaptopyridine-modified gold nanoparticles by surface-enhanced Raman scattering

HPTLC-MS analysis of melamine in milk: standardization and validation

Rapid and High-Throughput Determination of Melamine in Milk Products and Eggs by Full Automatic On-line Polymer Monolith Microextraction Coupled to High-Performance Liquid Chromatography

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