A new method for the determination of electrical properties of supported bilayer lipid membranes by cyclic voltammetry

Supported Bilayer Lipid Membranes as Ion and Molecular Probes

“…The previous report using LAPS technique has studied the system by consideration of the solution impedance affection. 42 However the value is not so high that it approaches to the R m . Because in our previous research the non-membrane resistance was not found so high in conventional planar BLM, and the technique for forming self-organized s-BLMs is based conceptually on interactions between a nascent metallic surface and amphipathic lipid molecules, it is supposed that a very possible principle composition of R n is aroused by the metal-lipid interface (interface resistance).…”

“…The most important feature is that, when R n reach the same order as R n (≈R m ), the voltammogram does not display the shape of parallelogram any longer, and it is very similar to that observed for the s-BLMs system. 42 The intercept with the i axis drops down sharply. The slope decreases during potential sweeping, and it is much lower than the slope in the case of BLM-simulater without R n or with a low value of R n .…”

“…42 A detailed observation of the effect of R n on the shape of I/V cycle is carried out. In our experiment, R n varies in a wide range from 10 0 to 10 9 Ω, while the other membrane properties are simulated holding R m at 10…”

“…To make clear the effect of R n on the I-V response, the quantitative relationship of the membrane current with the sweep wave potential should be derived rigorously from the solution of coupled equations based on the equivalent circuit. 42 The set of differential equations is as follows:…”

“…In this section, an unusual method is proposed which can determine the properties accurately without any iteration. 42 So the real-time measurement can be carried for the dynamic analysis.…”