A new method for the determination of virus specific IgG and IgM antibodies

A newly developed ion exchange chromatography is reported; it is a simple and quick method to separate a highly purified IgM fraction from blood serum. The immunofluorescence test was used to demonstrate that these fractions contain only virus-specific IgM antibodies, but no demonstrable IgG antibodies, which can often block the specific IgM reactivity by competitive inhibition. The specificity of the reaction was tested on sera from 145 persons: the IgM test was always positive in clinically proven viral infections; the reaction was negative in all patients whose infection had occurred some time in the past. A series of suspected virus infections could be verified by the demonstration of specific IgM antibodies.

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Eine Schnellmethode zur Bestimmung virusspezifischer IgM-Antikörper

Goetz

Peller

Oettrich

1982

Infection

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Der Latex-IgM-Test in der Virusdiagnostik : Bestimmung von Zytomegalie-Antikörpern

Schmidt

Klein

Brade

1981

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. 1. A

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Infection with Cytomegalovirus During Pregnancy: Specific IgM Antibodies as a Marker of Recent Primary Infection

Griffiths¹,

Stagno²,

Pass³

et al. 1982

Journal of Infectious Diseases

121

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Specific IgM antibodies that persisted for up to four months were detected by radioimmunoassay (RIA) in the sera of 16 (55%) of 29 women with primary infections due to cytomegalovirus (CMV). The RIA for IgM detected primary infections in six (86%) of seven sera obtained within four months of seroconversion. In contrast, IgM antibodies were never detected by RIA in 104 serum samples from 18 women with recurrent infections due to CMV, irrespective of whether intrauterine transmission of virus had occurred. Specific IgM antibodies were also detected in the earliest samples during pregnancy of serum from three (14%) of 21 women whose type of infection with CMV was unknown but who had been delivered of congenitally infected infants. All of these results show that primary infection with CMV in the first trimester of pregnancy can be diagnosed by testing a single serum sample by RIA for IgM antibodies. Attempts to measure IgM antibodies by immunofluorescence gave a high frequency (19 [18%] of 104) of false-positive reactions.

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A new method for the determination of virus specific IgG and IgM antibodies

Cited by 3 publications

References 3 publications

Eine Schnellmethode zur Bestimmung virusspezifischer IgM-Antikörper

Eine Schnellmethode zur Bestimmung virusspezifischer IgM-Antikörper

Der Latex-IgM-Test in der Virusdiagnostik : Bestimmung von Zytomegalie-Antikörpern

Infection with Cytomegalovirus During Pregnancy: Specific IgM Antibodies as a Marker of Recent Primary Infection

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