A new method for the size estimation of the RNA genome segments of influenza virus

Sleigh, M.J.; Both, Gerald W.; Brownlee, George G.

doi:10.1093/nar/6.4.1309

Cited by 43 publications

(32 citation statements)

References 38 publications

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“…On this basis, we propose that RNP a may be a triplet and RNP c a doublet; the proposed relationship between RNPs and RNA segments is shown. We have also calculated the relative molar abundance of the RNPs, based on their relative amounts and the length of each RNA segment expressed as the number of nucleotides (Sleigh et al, 1979). The values obtained show the large RNPs to be present in less than equimolar amounts and are in general agreement with the values published for the RNA segments (McGeoch et al, 1976).…”

Section: Assignment Of Rna Segments To Rnp Size Classessupporting

confidence: 79%

“…Band separation was greater at lower * RNPs were located by autoradiography, excised from the gel, and Cerenkov radiation was measured. t The molar representation of each RNP band was calculated relative to RNP e from the 3zp content of the RNP bands and from the size of the RNA segments (Sleigh et aL, 1979). Values are compared with those for the RNA of FP/R (McGeoch et aL, 1976).…”

Section: Electrophoretic Separation Of Rnpsmentioning

confidence: 99%

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Electrophoretic Separation of Influenza Virus Ribonucleoproteins

Rees

Dimmock

1981

Journal of General Virology

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SUMMARYA new procedure for the separation of influenza virus particle ribonucleoproteins (RNPs) by electrophoresis on a slab of polyacrylamide gel resolves five discrete bands. The largest is a triplet containing the three largest RNAs (1 to 3), the intermediate sized RNP is a doublet containing RNAs 5 and 6 and the others each contain a single RNA. In addition, each RNP is composed of NP protein and an amount of M which is independent of the size of the RNP. This suggests that some virus particle M is specifically associated with influenza ribonucleoprotein.

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Section: Assignment Of Rna Segments To Rnp Size Classessupporting

confidence: 79%

Section: Electrophoretic Separation Of Rnpsmentioning

confidence: 99%

Electrophoretic Separation of Influenza Virus Ribonucleoproteins

Rees

Dimmock

1981

Journal of General Virology

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“…However, this possibility depends on there being sufficient nucleotides in segment 8 to code for the two polypeptides. Recent estimates of the size of RNA segment 8 from cDNA copies suggest that it contains 890-970 nucleotides (37,38). NS1 has been estimated to have a molecular weight of nt23,000, and NS2 -11,000 (7,14).…”

Section: Discussionmentioning

confidence: 99%

Segment 8 of the influenza virus genome is unique in coding for two polypeptides.

Lamb¹,

Choppin²

1979

Proc. Natl. Acad. Sci. U.S.A.

138

111

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In previous studies we showed that a ninth polypeptide with a molecular weight of t11,000 (NS2) found in influenza virus-infected cells was unique, that it could be synthesized in vitro, and that its expression in vivo required early protein synthesis. On the basis of these results we suggested that one of the eight genome RNA segments of influenza virus codes for two polypeptides [Lamb, R. A., Etkind, P. R. & Choppin, P.W. (1978) Virology 91, 60-78]. We describe here differences in the electrophoretic mobility of the NS2 polypeptides of different strains of influenza A virus. These results provided further evidence that NS2 is virus coded and also made possible genetic studies using recombinants between two virus strains (HK and PR8) whose NS2 polypeptides differ. These studies showed that the gene for NS2 reassorts with that of the nonstructural polypeptide NS,, which is coded by genome segment 8. A mRNA for NS2 has been separated from that of NS1 and the other viral polypeptides by centrifugation and has been translated in vitro.Hybridization of genome segment 8 to the total mRNAs from infected cells specifically prevented the synthesis of NS2 and NS1. These results indicate that influenza virus genome segment 8 is transcribed into two separate mRNAs that code for two polypeptides, NS1 and NS2. Possible mechanisms for the transcription of the two mRNAs from either contiguous or overlapping genes are discussed.

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“…Influenza virus has a segmented genome of eight single-stranded RNAs [1] totalling about 14,000 nucleotides [2,3]. Each gene has a region of 12 nucleotides in common with the others at the 3' end, and another region of 13 nucleotides in common at the 5' end [4,5].…”

Section: Introductionmentioning

confidence: 99%

The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment 8 of influenza virus (A/PR/8/34)

et al. 1981

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Complete double-stranded DNA copies of the RNA genes of the human influenza virus A/PR/8/34 have been synthesized by using two synthetic oligodeoxynucleotide primers. The gene encoding the non-structural proteins NS1 and NS2, prepared with these primers, has been cloned into the bacteriophage M13mp7 and sequenced. The sequence is compared with that from another human strain and from an avian strain.

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A new method for the size estimation of the RNA genome segments of influenza virus

Cited by 43 publications

References 38 publications

Electrophoretic Separation of Influenza Virus Ribonucleoproteins

Electrophoretic Separation of Influenza Virus Ribonucleoproteins

Segment 8 of the influenza virus genome is unique in coding for two polypeptides.

The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment 8 of influenza virus (A/PR/8/34)

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