A New Method for the Determination of Sucrose Concentration in a Pure and Impure System: Spectrophotometric Method

Borji, Abdelali; Borji, Fz; Jourani, A.

doi:10.1155/2017/8214120

Cited by 12 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protease (EC 3.4.21) activity was measured using the Folin-phenol method ( McDonald and Chen, 1965 ); amylase (EC 3.2.1.1) activity was measured using the starch-iodine method ( Xiao et al, 2006 ); lipase (EC 3.1.1.3) activity was measured using the polyvinyl alcohol olive oil emulsion hydrolysis method ( Andersson, 1980 ); cellulase (EC 3.2.14) activity was measured using the 3,5-dinitrosalicylic acid method ( Breuil and Saddler, 1985 ). The protein content was measured using the Coomassie brilliant blue dual-wavelength method ( Sedmak and Grossberg, 1977 ); the glucose content was measured using the 3,5-dinitrosalicylic acid method ( Borji et al, 2017 ). A suitable amount of freeze-dried feces was mixed with five times of its weight of sterile water, and then it was used to measure the pH.…”

Section: Methodsmentioning

confidence: 99%

The effects of food provisioning on the gut microbiota community and antibiotic resistance genes of Yunnan snub-nosed monkey

Li,

Jing,

Tang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Yunnan snub-nosed monkeys (Rhinopithecus bieti) are the highest elevation lived non-human primate, and their survival has been threatened for decades. To promote their population growth, a reserve provides a typical monkey population with supplemental food. However, the influences of this food provisioning on their gut microbiota and antibiotic resistance genes (ARGs) were unknown. Therefore, we investigated the gut microbiota and ARGs of the food-provisioned monkey population compared with another wild foraging population. We found that food provisioning significantly increased the gut microbiota diversity and changed the community composition, particularly increased both the Firmicutes abundance and Firmicutes/Bacteroidetes ratio. Meanwhile, the food provisioning decreased the complex and stable gut microbiota network. KEGG functions were also influenced by food provisioning, with wild foraging monkeys showing higher functions of metabolism and genetic information processing, especially the carbohydrate metabolism, while food-provisioned monkeys exhibited increased environmental information processing, cellular processes, and organismal systems, including valine, leucine, and isoleucine degradation. In addition, food provisioning increased the abundance of ARGs in the gut microbiota, with most increasing the abundance of bacA gene and changing the correlations between specific ARGs and bacterial phyla in each population. Our study highlights that even food provisioning could promote wildlife nutrient intake, and it is necessary to pay attention to the increased ARGs and potential effects on gut microbiota stability and functions for this human conservation measure.

show abstract

Section: Methodsmentioning

confidence: 99%

The effects of food provisioning on the gut microbiota community and antibiotic resistance genes of Yunnan snub-nosed monkey

Li,

Jing,

Tang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Sucrose and fructose contents were determined according to Borji et al [38]. Sucrose synthase (SS), sucrose phosphate synthase (SPS), neutral invertase (NI), and acid invertase (AI) activity were all determined with kits (purchased from Beijing Solarbio Technology Co., Ltd., Beijing, China).…”

Section: Determination Of Biochemical Indices Relating To Fruit Qualitymentioning

confidence: 99%

Improving the Yield and Quality of Tomato by Using Organic Fertilizer and Silicon Compared to Reducing Chemical Nitrogen Fertilization

Zhang,

Liu,

Liang

et al. 2024

Agronomy

View full text Add to dashboard Cite

Essential macronutrient nitrogen (N) is crucial for plant growth and yield, but excessive chemical N fertilizer not only increases unnecessary production costs but also causes environmental pollution. Therefore, reducing N fertilizer use by increasing organic fertilizer use is crucial for sustainable agriculture. In this study, we investigated the effects of three nitrogen levels—the recommended rate (N), a 20.0% reduced rate (0.8N), and a 40.0% reduced rate (0.6N)—and two levels of organic fertilizer—a normal dose (M) and a four-times the normal dose (4M)—combined with root application of the beneficial element silicon (Si) on the photosynthetic characteristics, yield, and fruit quality of the tomato cultivar ‘Tianxi No. 5’. Compared with M + N treatment, the longitudinal diameter, transverse diameter, fruit weight, and fruit yield of tomato fruit in 4M + 0.6N treatment significantly increased by 12.4%, 14.6%, 14.5%, and 12.8%, respectively, while the yield was further improved with Si application. In addition, a reduction in N fertilizer and an increase in organic fertilizer, combined with Si application, improved fruit quality parameters such as concentrations of vitamin C, lycopene, phenols, flavonoids, sucrose, fructose, etc., and promoted sugar metabolism-related enzyme activity (sucrose synthase, invertase, and sucrose phosphate synthase) and the accumulation of N in the fruit. The principal component analysis and three-factor analysis of variance (ANOVA) of the fruit quality and yield indices showed that nitrogen fertilizer, organic fertilizer, silicon fertilizer, and the interaction of the three had significant effects on the quality and yield of tomato fruits, and that the 4M + 0.6N + Si treatment had the best combined effect on the yield and quality of the tomatoes. Thus, a moderate reduction in chemical N fertilizer, combined with increased organic fertilizer and Si, could be an effective agronomic practice for improving the yield and quality of tomatoes.

show abstract

“…Soluble, insoluble, and total carbohydrates were estimated by reaction with cupric ions and arsenomolybdate reagent [22]. Sucrose was calorimetrically estimated using the method described by [23], while trehalose was assayed by its conversion to glucose with trehalase, measured using a glucose assay kit [24]. Amylase (3.2.1.1) was extracted using sodium acetate buffer (pH 5.5), following the method outlined by [25], and then assayed using nonsolubilized starch, as per the method described by [26].…”

Section: Estimation Of Sugars Content and Photosynthetic Pigmentsmentioning

confidence: 99%

Allelopathic Impact of Callistemon Citrinus on Sorghum Bicolor Growth under Salinity Stress

ElAal,

Mona

2024

Asian J. Res. Crop Sci.

View full text Add to dashboard Cite

The current study assessed the impact of 0.09% aqueous leaf extract from Callistemon citrinus on the growth of two cultivars of Sorghum bicolor L. (Giza 15 - G15 and Dorado - Dor) under two salinity levels (100 mM and 200 mM NaCl). Results revealed that salinity reduced various growth criteria, including insoluble and total carbohydrates, photosynthetic pigments, protein, total phenols, and reduced glutathione, while soluble carbohydrates (sucrose and trehalose) were influenced. Salinity also affected free amino acids (proline and glycine betaine), malondialdehyde, and ascorbic acid. The activity of amylase decreased, while antioxidant enzymes (catalase, peroxidase, superoxide dismutase, ascorbic acid oxidase, and glutathione peroxidase) increased. Additionally, concentrations of K+, Ca++, and Mg++ were reduced, while Na+ accumulated. Application of Callistemon extract improved plant growth under stressed or normal growth conditions. The aqueous leaf extract (0.09%) of Callissstemon sp., with its enriched phenolic allelochemicals, mitigated the impact of salinity by enhancing photosynthesis, osmoregulation, and the antioxidant defense system of sorghum plants. Furthermore, results indicated that Sorghum cultivar G15 exhibited more resistance than Dor, with this resistance correlating with the activity of the antioxidant system.

show abstract

A New Method for the Determination of Sucrose Concentration in a Pure and Impure System: Spectrophotometric Method

Cited by 12 publications

References 12 publications

The effects of food provisioning on the gut microbiota community and antibiotic resistance genes of Yunnan snub-nosed monkey

The effects of food provisioning on the gut microbiota community and antibiotic resistance genes of Yunnan snub-nosed monkey

Improving the Yield and Quality of Tomato by Using Organic Fertilizer and Silicon Compared to Reducing Chemical Nitrogen Fertilization

Allelopathic Impact of Callistemon Citrinus on Sorghum Bicolor Growth under Salinity Stress

Contact Info

Product

Resources

About