A New Method for Visualizing Drusen and Their Progression in Flood-Illumination Adaptive Optics Ophthalmoscopy

Rossi, Ethan A.; Norberg, Nathaniel; Eandi, Chiara M; Chaumette, Céline; Kapoor, Saloni; Le, Laura; Snyder, Valerie C.; Martel, Joseph N.; Gautier, Josselin; Gocho, Kiyoko; Dansingani, Kunal K.; Chhablani, Jay; Arleo, Angelo; Mrejen, Sarah; Sahel, José‐Alain; Grieve, Kate; Pâques, Michel

doi:10.1167/tvst.10.14.19

Cited by 9 publications

(15 citation statements)

References 30 publications

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“…In order to understand and quantify further the role of these structural markers on functional vision, we mapped these drusen onto the retina. To do so, we used a recently developed enface drusen identification method (Rossi 2021[22]).…”

Section: Discussionmentioning

confidence: 99%

“…The important aspect today is to detect them at the earliest possible moment to track its progression and understand its development. That is why the emergence of recent imaging methods that allow the precise visualization of small drusen is crucial, such as retromode imaging (Cozzi et al 2023[52]) or gaze-dependent imaging (Rossi et al 2021[22]) that can both detect smaller drusen before a classic SD-OCT analysis. Our findings show that those small drusen could also be detected by looking at the changes in fixational eye movements during a fixation task.…”

Section: Discussionmentioning

confidence: 99%

“…The latest group was renamed “drusen group” as the only certainty was the presence of foveal drusen, first revealed by OCT imaging. Before the beginning of the experiment, each participant from the older controls and the drusen group were imaged using a commercial flood-illumination adaptive optics ophthalmoscope (rtx1, Imaging Eyes) and following the gaze-dependent protocol described by Rossi et al (Rossi 2021[22]). This allowed for a more precise visualization of the drusen in the foveal region.…”

Section: Methodsmentioning

confidence: 99%

“…However, in order to get more precise information about the position and size of the drusen in the fovea, gaze-dependent imaging was performed on every participant from the older adults and the drusen groups using the same commercial FIAO device (rtx1, Imagine Eyes, Orsay, France). It is an imaging protocol which uses FIAO to reveal drusen with high contrast and resolution (Rossi 2021 [22]). The method allows for the visualization of small drusen (<63µm) in the fovea (zone covering 2° radius around the center of the fovea) better than previously known methods.…”

Section: Gaze-dependent Imaging and Drusen Visualizationmentioning

confidence: 99%

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Foveolar drusen decrease fixation stability in pre-symptomatic AMD

Murari,

Gautier,

Daout

et al. 2023

Preprint

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PurposeWe conducted a clinical study to characterize fine spatiotemporal alterations of FEM in controls and patients with pre-clinical signs of age-related macular degeneration (AMD).MethodsTwenty-seven participants were recruited from the SilverSight cohort of the Vision Institute - Quinze-Vingts National Vision Hospital, Paris. Subjects were included in three groups: healthy young, healthy aging controls, and aging subjects with drusen. Detection of microdrusen was done using gaze-dependent adaptive optics ophthalmology. Monocular retinal tracking was based on adaptive optics flood illumination ophthalmoscopy (AO-FIO). The system allows for sub-arcminute resolution, high-speed, distortion-free imaging of the foveal area. Local stimulation through digital micromirror (DMD) projection from a near infrared source enabled projection of optotypes. Eye movements were estimated using a phase-correlation registration algorithm.ResultsCompared to controls, patients with foveal drusen showed higher microsaccade amplitude, higher drift diffusion coefficient, and worse fixation stability -i.e. higher isoline area. Among the drusen group, the increase of microsaccade amplitude and ISOA was correlated with drusen eccentricity; the closer the drusen were to the center of the fovea, the more unstable were FEM.ConclusionsWe demonstrate the clinical interest of a novel high-precision retinal tracking technique to characterize FEM changes at the microscopic level. We found significant differences between the drusen group and both healthy young and older adult groups. Central drusen altered fixation stability characteristics, resulting in compensatory FEM changes. High speed, high resolution FEM analysis may help to define the functional signatures of early age-related changes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Gaze-dependent Imaging and Drusen Visualizationmentioning

confidence: 99%

See 2 more Smart Citations

Foveolar drusen decrease fixation stability in pre-symptomatic AMD

Murari,

Gautier,

Daout

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Use of single cell transcriptomics [300], anti-sense oligonucleotides [152], antibodies directed at inflammatory mediators [301], and use of specific micro-RNAs to lower IOP and perhaps provide neuroprotection [149] by exploiting recently discovered cellular nanotubes [302] are exciting new developments worthy of further pursuit. Last but not least, novel imaging technologies using 2-photon-excited fluorescence and flood-illumination adaptive optics [303,304], coupled with proteostasis drugs to eliminate excess phosphorylated proteins and misfolded proteins [305,306] from the ANC and from the retina/optic nerve/brain offer new treatment paradigms for cOHT/POAG/NTG/GON, especially if the combinatorial treatments [257,307] are reproduced in other clinical trials across the world.…”

Section: Novel Techniques and Technologies To Help Preserve Eyesightmentioning

confidence: 99%