A new method to effectively and rapidly generate neurons from SH-SY5Y cells

Abstract

It is well known that neurons differentiated from SH-SY5Y cells can serve as cell models for neuroscience research; i.e., neurotoxicity and tolerance to morphine in vitro. To differentiate SH-SY5Y cells into neurons, RA (retinoic acid) is commonly used to produce the inductive effect. However, the percentage of neuronal cells produced from SH-SY5Y cells is low, either from the use of RA treatment alone or from the combined application of RA and other chemicals. In the current study, we used CM-hNSCs (conditioned medium of human neural stem cells) as the combinational inducer with RA to prompt neuronal differentiation of SH-SY5Y cells. We found that neuronal differentiation was improved and that neurons were greatly increased in the differentiated SH-SY5Y cells using a combined treatment of CM-hNSCs and RA compared to RA treatment alone. The neuronal percentage was higher than 80% (about 88%) on the 3rd day and about 91% on the 7th day examined after a combined treatment with CM-hNSCs and RA. Cell maturation and neurite growth of these neuronal cells were also improved. In addition, the use of CM-hNSCs inhibited the apoptosis of RA-treated SH-SY5Y cells in culture. We are the first to report the use of CM-hNSCs in combination with RA to induce neuronal differentiation of RA-treated SH-SY5Y cells. Our method can rapidly and effectively promote the neuronal production of SH-SY5Y cells in culture conditions.

Neural stem cell-conditioned medium ameliorates Aβ25–35-induced damage in SH-SY5Y cells by protecting mitochondrial function

“…However, in recent years, NSC-CDM has received increasing attention due to the extensive study of the bystander behavior of NSCs in vivo, particularly that of the microvesicles released by NSCs. In addition, NSC-CDM has been shown to exert anti-apoptotic effects both in vitro [24] and in vivo [11]. To circumvent these potential obstacles and find new therapeutic strategies to treat AD, we first explored the effect of NSC-CDM in Aβ 25-35 -induced SH-SY5Y cells and we consider that NSC-CDM may be an effective treatment for AD.…”

General Anesthetics Regulate Autophagy via Modulating the Inositol 1,4,5-Trisphosphate Receptor: Implications for Dual Effects of Cytoprotection and Cytotoxicity

“…To differentiate neurons, 0.5ml SH-SY5Y cells were seeded from a confluent T75 into a 6-well plate, and 10µM retinoic acid (RA) and B27 were added into the medium, which was changed every two days. Under those conditions, neurons could be obtained after one week 54 .…”

The SH-SY5Y cell line in Parkinson’s disease research: a systematic review

“…To obtain derivative cells with a neuronal phenotype, multiple differentiation protocols have been described [21–25], but details about the final population of cells, regarding the fate-choices and fate-specification of cells that undergo terminal differentiation, have not been systematically reported. Since PD is characterized by the death of SN DAergic neurons, the degree to which this cell line displays a DAergic phenotype is a key aspect regarding the validity of the model.…”