A New Microengineered Platform for 4D Tracking of Single Cells in a Stem‐Cell‐Based In Vitro Morphogenesis Model

Samal, Pinak; Maurer, Philipp; Blitterswijk, Clemens van; Truckenmüller, Roman; Giselbrecht, Stefan

doi:10.1002/adma.201907966

Cited by 15 publications

(18 citation statements)

References 70 publications

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“… 580 Another system for real-time cell assessment is the so-called 4D cell culture imaging systems, 581 which are able to track cell migration, matrix remodeling, proliferation, and phenotypical changes in a spatial and temporal manner. Samal et al 582 have recently presented a microengineered high-content imaging platform for 4D tracking of single cells in an in vitro morphogenesis model. The platform fabrication used a free-forming variant of negative microscale pressure thermoforming, where a transparent polycarbonate film was patterned into microcavities arranged in an array fashion, with a ∼500 μm width and a ∼500–1250 μm length.…”

Section: Microarray Strategies Applied In Biomaterials Screening and Used To Model The Cellular Microenvironmentmentioning

confidence: 99%

“…The platform fabrication used a free-forming variant of negative microscale pressure thermoforming, where a transparent polycarbonate film was patterned into microcavities arranged in an array fashion, with a ∼500 μm width and a ∼500–1250 μm length. 582 The thin-walled cavities allowed for multidimensional time-lapse imaging using confocal microscopy, translatable to many common laboratories. The authors created a data analysis pipeline using free software that could be used to evaluate and quality-control single-cell migration and aggregate morphogenesis, making this time/spatial single-cell tracking method an open-access resource.…”

Section: Microarray Strategies Applied In Biomaterials Screening and Used To Model The Cellular Microenvironmentmentioning

confidence: 99%

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High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

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The complex interaction of cells with biomaterials (i.e., materiobiology) plays an increasingly pivotal role in the development of novel implants, biomedical devices, and tissue engineering scaffolds to treat diseases, aid in the restoration of bodily functions, construct healthy tissues, or regenerate diseased ones. However, the conventional approaches are incapable of screening the huge amount of potential material parameter combinations to identify the optimal cell responses and involve a combination of serendipity and many series of trial-and-error experiments. For advanced tissue engineering and regenerative medicine, highly efficient and complex bioanalysis platforms are expected to explore the complex interaction of cells with biomaterials using combinatorial approaches that offer desired complex microenvironments during healing, development, and homeostasis. In this review, we first introduce materiobiology and its high-throughput screening (HTS). Then we present an in-depth of the recent progress of 2D/3D HTS platforms (i.e., gradient and microarray) in the principle, preparation, screening for materiobiology, and combination with other advanced technologies. The Compendium for Biomaterial Transcriptomics and high content imaging, computational simulations, and their translation toward commercial and clinical uses are highlighted. In the final section, current challenges and future perspectives are discussed. High-throughput experimentation within the field of materiobiology enables the elucidation of the relationships between biomaterial properties and biological behavior and thereby serves as a potential tool for accelerating the development of high-performance biomaterials.

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Section: Microarray Strategies Applied In Biomaterials Screening and Used To Model The Cellular Microenvironmentmentioning

confidence: 99%

Section: Microarray Strategies Applied In Biomaterials Screening and Used To Model The Cellular Microenvironmentmentioning

confidence: 99%

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

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“…[246] These membrane-based confinement devices have been used to study morphogenesis in early embryonic development (Figure 3d). [247] Moreover, thermoformed membrane-based microwell devices have been used to culture pancreatic islets. [248,249] A similar membrane-based carrier will allow extrahepatic implantation of these islets in the not too distant future.…”

Section: Biomaterials For Cell Aggregation and Confinementmentioning

confidence: 99%

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Guttenplan

Birgani

Giselbrecht

et al. 2021

Adv Healthcare Materials

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In recent years, the use of microfabrication techniques has allowed biomaterials studies which were originally carried out at larger length scales to be miniaturized as so-called "on-chip" experiments. These miniaturized experiments have a range of advantages which have led to an increase in their popularity. A range of biomaterial shapes and compositions are synthesized or manufactured on chip. Moreover, chips are developed to investigate specific aspects of interactions between biomaterials and biological systems. Finally, biomaterials are used in microfabricated devices to replicate the physiological microenvironment in studies using so-called "organ-on-chip," "tissue-on-chip" or "disease-on-chip" models, which can reduce the use of animal models with their inherent high cost and ethical issues, and due to the possible use of human cells can increase the translation of research from lab to clinic. This review gives an overview of recent developments at the interface between microfabrication and biomaterials science, and indicates potential future directions that the field may take. In particular, a trend toward increased scale and automation is apparent, allowing both industrial production of micron-scale biomaterials and high-throughput screening of the interaction of diverse materials libraries with cells and bioengineered tissues and organs.

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“…Further, designing novel multiplexed microfluidic assays and largescale, high-throughput screening platforms compatible with the embryo models will help to learn more about how biological and environmental factors affect development. [193] The success of synthetic embryology also depends on the development of new imaging techniques, such as 4D live cell imaging [194] or light sheet microscopy, and how well these imaging techniques are compatible with the increasing complexity of the engineered microenvironments. Computational resources to validate the findings of the in vitro system to the in vivo scenario and computational modeling have already taken an important position in ensuring the progress of this field to keep up with new technologies.…”

Section: Wwwadvancedsciencenewscom Wwwadvancedsciencecommentioning

confidence: 99%

From Snapshots to Development: Identifying the Gaps in the Development of Stem Cell‐based Embryo Models along the Embryonic Timeline

et al. 2021

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In recent years, stem cell-based models that reconstruct mouse and human embryogenesis have gained significant traction due to their near-physiological similarity to natural embryos. Embryo models can be generated in large numbers, provide accessibility to a variety of experimental tools such as genetic and chemical manipulation, and confer compatibility with automated readouts, which permits exciting experimental avenues for exploring the genetic and molecular principles of self-organization, development, and disease. However, the current embryo models recapitulate only snapshots within the continuum of embryonic development, allowing the progression of the embryonic tissues along a specific direction. Hence, to fully exploit the potential of stem cell-based embryo models, multiple important gaps in the developmental landscape need to be covered. These include recapitulating the lesser-explored interactions between embryonic and extraembryonic tissues such as the yolk sac, placenta, and the umbilical cord; spatial and temporal organization of tissues; and the anterior patterning of embryonic development. Here, it is detailed how combinations of stem cells and versatile bioengineering technologies can help in addressing these gaps and thereby extend the implications of embryo models in the fields of cell biology, development, and regenerative medicine.

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A New Microengineered Platform for 4D Tracking of Single Cells in a Stem‐Cell‐Based In Vitro Morphogenesis Model

Cited by 15 publications

References 70 publications

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

From Snapshots to Development: Identifying the Gaps in the Development of Stem Cell‐based Embryo Models along the Embryonic Timeline

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