A new microsporidium, Vairimorpha subcoccinellae n. sp. (Microsporidia: Burenellidae), isolated from Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae)

Baki, Hilal; Bekircan, Çağrı

doi:10.1016/j.jip.2017.12.004

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…Microsporidia are common pathogens in a wide range of insect taxa [6,8,14]. More than 100 microsporidia species have been identified in the Insecta until now, while almost 1 in 5 of them have been recorded from only lepidopteran [13].…”

Section: Conclusion and Discussionmentioning

confidence: 99%

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A New Microsporidian Pathogen of the Mediterranean Flour Moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

Bekircan¹,

Tosun²

2021

Biological Diversity and Conservation

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In this study, a new Vairimorpha isolate was defined from Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) in Turkey (Malatya). Infection was determined in the host's hindgut, both larval and adult stages. Two types spore originated from the polymorphic sporulation was detected during the observations. While the binucleate ovocylindrical fresh spores were measured 4.38 ± 0.31 μm (5.09-3.77 μm, n = 100) in length and 2.80 ± 0.18 μm (3.21-2.43 μm, n=100) in width, the ovoid meiospores were measured as 3.50 ± 0.46 μm (4.98-2.51 μm, n = 100) in length and 2.02 ± 0.28 μm (2.94-1.50 μm, n=100) in width. The multilocus genetic analyses based on SSU rRNA and RPB1 genes were carried for determining the phylogenetic status of the current microsporidium. In ML and BI trees, the current microsporidium was separated into a different branch from V. necatrix strains within the Vairimorpha clade

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Section: Conclusion and Discussionmentioning

confidence: 99%

“…In recent years, other combat techniques such as microbial control have attracted attention in pest control. Entomopathogenic organisms that cause infection in pests such as E. kuehniella frequently recorded in the literature, and there is increasing interest in isolating and identifying these organisms [6,7,8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

A New Microsporidian Pathogen of the Mediterranean Flour Moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

Bekircan¹,

Tosun²

2021

Biological Diversity and Conservation

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“…For transmission electron microscopy (TEM), infected tissues were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) for 1–2 h, washed with cacodylate buffer and postfixed in 1% aqueous OsO 4 for 2 h. After postfixation, the tissues were washed with cacodylate buffer and dehydrated through an ascending alcohol series and acetone before embedding in Spurr's resin (Spurr, 1969; Baki and Bekircan, 2018). Thin sections were taken with Leica EM UC7 ultramicrotome and mounted on Pioloform-coated copper grids which were then stained with saturated uranyl acetate and Reynolds’ lead citrate (Reynolds, 1963).…”

Section: Methodsmentioning

confidence: 99%

A new isolate ofNosema fumiferanae(Microsporidia: Nosematidae) from the date mothApomyelois(Ectomyelois)ceratoniae, Zeller, 1839 (Lepidoptera: Pyralidae)

Tosun¹

2020

Parasitology

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In this study, a microsporidian pathogen of the date moth (Apomyelois (Ectomyelois) ceratoniae, Zeller, 1839) also known as the carob moth, is described based on light microscopy, ultrastructural characteristics and comparative molecular analysis. The pathogen infects the gut and hemolymph of A. ceratoniae. All development stages are in direct contact with the host cell cytoplasm. Fresh spores with nuclei arranged in a diplokaryon are oval and measured 3.29 ± 0.23 μm (4.18–3.03 μm, n = 200) in length and 1.91 ± 0.23 μm (2.98–1.66 μm, n = 200) in width. Spores stained with Giemsa's stain measured 3.11 ± 0.31 μm (3.72–2.41 μm, n = 150) in length and 1.76 ± 0.23 μm (2.16–1.25 μm, n = 150) in width. Spores have an isofilar polar filament with 10-12 coils. An 1110 bp long alignment of the current microsporidium showed an SSU rRNA gene difference of only 0.0009, corresponding to >99.91% sequence similarity with Nosema fumiferanae, while RPB1 gene sequences were 98.03% similar within an alignment of 969 bp. All morphological, ultrastructural and molecular features indicate that the microsporidian pathogen of A. ceratoniae is the new isolate of the N. fumiferanae and is named here as Nosema fumiferanae TY61.

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“…To date, microsporidia are the most-studied protist-like insect parasites [ 111 ] and, in the last 150 years, around 1500 species in 200 genera have been described, with nearly half of them from insect hosts [ 112 ]. New microsporidia species are being discovered each year in different insect hosts, and more are being uncovered by molecular environmental sampling techniques [ 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 ]. Jaroslav Weiser, with his extensive experience researching microsporidia parasites, suggested that there might be a microsporidian in every living invertebrate species [ 121 ].…”

Section: Protist Parasites Including Microsporidia Within the Eu Rear...mentioning

confidence: 99%

Protists in the Insect Rearing Industry: Benign Passengers or Potential Risk?

Bessette

Williams

2022

Insects

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As the insects for food and feed industry grows, a new understanding of the industrially reared insect microbiome is needed to better comprehend the role that it plays in both maintaining insect health and generating disease. While many microbiome projects focus on bacteria, fungi or viruses, protists (including microsporidia) can also make up an important part of these assemblages. Past experiences with intensive invertebrate rearing indicate that these parasites, whilst often benign, can rapidly sweep through populations, causing extensive damage. Here, we review the diversity of microsporidia and protist species that are found in reared insect hosts and describe the current understanding of their host spectra, life cycles and the nature of their interactions with hosts. Major entomopathogenic parasite groups with the potential to infect insects currently being reared for food and feed include the Amoebozoa, Apicomplexa, Ciliates, Chlorophyta, Euglenozoa, Ichtyosporea and Microsporidia. However, key gaps exist in the understanding of how many of these entomopathogens affect host biology. In addition, for many of them, there are very limited or even no molecular data, preventing the implementation of molecular detection methods. There is now a pressing need to develop and use novel molecular tools, coupled with standard molecular diagnostic methods, to help unlock their biology and predict the effects of these poorly studied protist parasites in intensive insect rearing systems.

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A new microsporidium, Vairimorpha subcoccinellae n. sp. (Microsporidia: Burenellidae), isolated from Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae)

Cited by 11 publications

References 26 publications

A New Microsporidian Pathogen of the Mediterranean Flour Moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

A New Microsporidian Pathogen of the Mediterranean Flour Moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

A new isolate ofNosema fumiferanae(Microsporidia: Nosematidae) from the date mothApomyelois(Ectomyelois)ceratoniae, Zeller, 1839 (Lepidoptera: Pyralidae)

Protists in the Insect Rearing Industry: Benign Passengers or Potential Risk?

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