A new mitochondria-targeting fluorescent probe for ratiometric detection of H2O2 in live cells

Wang, Yuming; Wang, Yang; Wang, Guanyang; Huang, Chusen; Jia, Nengqin

doi:10.1016/j.aca.2019.11.024

Cited by 66 publications

(15 citation statements)

References 37 publications

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“…Recently, many reviews on SERS substrate materials have been published 13–19 . Compared with the above reviews, this study pays more attention to the design of substrate materials, that is, tuning the localized surface plasmon resonance position, assembling of nanoparticles, precise controlling of nanogaps.…”

Section: Introductionmentioning

confidence: 99%

Smart design of high‐performance surface‐enhanced Raman scattering substrates

Meng

Qiu

et al. 2021

SmartMat

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Surface‐enhanced Raman scattering (SERS) spectroscopy has renowned its fame for the ultra‐high sensitivity and single‐molecule detection ability, and listed as a fingerprint spectrum representative in various trace detection fields. Considerable efforts have been made by researchers to design high‐sensitive SERS‐active substrates ranging from noble metals to semiconductors. This review summarizes the fundamental theories for SERS technique, that is, the electromagnetic enhancement mechanism and chemical enhancement mechanism and the state‐of‐the‐art design strategies for noble metal and semiconductor substrates. It also sheds light on the effective approaches to improve the SERS activity for noble metal substrates, that is, tuning the localized surface plasmon resonance position, the assembling of hot spots, and precise controlling of nanogaps. Although charge transfer is considered as the main reason for the enhancement mechanism for semiconductors at the present stage, the underlying theoretical basis remains mysterious. This review summarized the critical points for SERS‐active substrates design and prospected the future development direction of SERS technology.

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Section: Introductionmentioning

confidence: 99%

Smart design of high‐performance surface‐enhanced Raman scattering substrates

Meng

Qiu

et al. 2021

SmartMat

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“…The mitochondria organelle signal activation ability of TAT was checked using commercially available mitochondrial dye Mito-Tracker Deep Red FM as a co-localization reference . The probe TAT and Mito-Tracker red were incubated with PC3 cells at 37 °C for 30 min and then incubated with 2.4 μM Zn 2+ for another 30 min.…”

Section: Resultsmentioning

confidence: 99%

Successive Detection of Zinc Ion and Citrate Using a Schiff Base Chemosensor for Enhanced Prostate Cancer Diagnosis in Biosystems

Muthusamy

Zhu

Rajalakshmi

et al. 2021

ACS Appl. Bio Mater.

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Sensitive and quantitative detection of prostate cancer (PC) requires a chemosensor with an applicable sensing strategy. A star-shaped Schiff base triaminoguanidine-integrated thiophene fluorophore TAT was rationally designed with nitrogen and sulfur atoms to coordinate with Zn 2+ as the initial step and to chelate with citrate as the following step. Formation of the complex TAT-Zn 2+ induced an intramolecular charge transfer and caused a red-shifted, Zn 2+ concentration-dependent fluorescence at 507 nm. Chelation of TAT-Zn 2+ with citrate led to an emission band at 692 nm upon an aggregation-induced emission mechanism. The distinctive fluorescence emissions of Zn 2+ and citrate biomarkers were demonstrated first in on-site paper-based test strips showing gradually enhanced colors at yellow and red channels and second in both in vitro and in vivo by using PC3 cells and BALB/c nude mouse animal models, respectively. The in vitro test confirmed the mitochondria organelle-targeting property of TAT, and the in vivo performance manifested the successful application of the probe in recognizing the prostate cancer. This is the first applicable chemosensor that could be in continuous recognition of dual PC biomarkers Zn 2+ and citrate in cancer diagnosis with a mitochondria organelle-targeting ability.

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“…[ 75–79 ] In addition, targeting ability can be achieved by combining the sensor with a specific cellular label or tagging molecule, whose emission can also be used for ratiometric sensing. [ 80–82 ]…”

Section: Sensing Techniquesmentioning

confidence: 99%

“…[75][76][77][78][79] In addition, targeting ability can be achieved by combining the sensor with a specific cellular label or tagging molecule, whose emission can also be used for ratiometric sensing. [80][81][82] Multiple emissions can be utilized to overcome the intrinsic limitations of certain sensors. For example, ROS probes are based on molecular compounds (e.g., boron dipyrromethene) that change their structure when they react (redox process) with these highly oxidative compounds.…”

Section: Sensing Techniquesmentioning

confidence: 99%

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Rodríguez‐Sevilla

Thompson

Jaque

2022

Advanced NanoBiomed Research

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Fluorescent microscopy has enabled the study of intracellular processes and revealed the most intricate details of the subcellular structure. This has benefitted not only the basic biological science, but also has had an impact in numerous biomedical applications. Basic fluorescent sensing techniques use the change in the absolute emission of a fluorescent sensor. This entails some disadvantages as the signal might be influenced by factors not directly related to the process under study (e.g., fluctuations in the excitation source). To overcome these drawbacks, one can use multiple emissions of a single or various fluorophores. There are numerous examples of multichannel fluorescence microscopy techniques that have given rise to numerous ratiometric methods and multiplexing assays. Herein, how the use of multiple emission channels has impacted fluorescence microscopy in terms of speed, sensitivity, and resolution is reviewed. Using recent examples, how the easy implementation of multichannel detection can overcome current limitations of the main used fluorescence techniques and promote the development of novel microscopy methods is shown.

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A new mitochondria-targeting fluorescent probe for ratiometric detection of H2O2 in live cells

Cited by 66 publications

References 37 publications

Smart design of high‐performance surface‐enhanced Raman scattering substrates

Smart design of high‐performance surface‐enhanced Raman scattering substrates

Successive Detection of Zinc Ion and Citrate Using a Schiff Base Chemosensor for Enhanced Prostate Cancer Diagnosis in Biosystems

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

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