1993
DOI: 10.1159/000238987
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A New Model Examining Intracellular and Extracellular Activity of Amoxicillin, Azithromycin, and Clarithromycin in Infected Cells

Abstract: An in vitro infection model was created using a suspension of macrophages, polymorphonuclear leukocytes, lymphocytes, fibroblasts, and human serum to which pathogen and antibiotic were added. Separate intracellular and extracellular antibiotic concentrations and activity against Staphylococcus aureus and Legionella pneumophila were assessed for three antimicrobial agents: amoxicillin, azithromycin and clarithromycin. Amoxicillin was found almost exclusively in extracellular fluid, where it was active; intracel… Show more

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Cited by 41 publications
(42 citation statements)
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“…THP-1 monocytes were heated at 60°C, treated for 30 min with 10% formalin, washed with RPMI-1640 twice and incubated with antibiotic, as indicated above. 6,9,13,14 Grepafloxacin was not found to be associated with these dead THP-1 monocytes. To determine the influence of temperature on antibiotic uptake by the monocytes, initial uptake studies were conducted from 0 to 2 h at 37°C and 0°C, where it was determined that FCS had no effect on grepafloxacin uptake.…”
Section: Methodsmentioning
confidence: 84%
“…THP-1 monocytes were heated at 60°C, treated for 30 min with 10% formalin, washed with RPMI-1640 twice and incubated with antibiotic, as indicated above. 6,9,13,14 Grepafloxacin was not found to be associated with these dead THP-1 monocytes. To determine the influence of temperature on antibiotic uptake by the monocytes, initial uptake studies were conducted from 0 to 2 h at 37°C and 0°C, where it was determined that FCS had no effect on grepafloxacin uptake.…”
Section: Methodsmentioning
confidence: 84%
“…Metronidazole and amoxicillin showed poor intracellular activity, as expected since they have been shown to have limited access to the intracellular space of phagocytic cells (17). However, the peak concentration of amoxicillin in serum is reported to vary from 5.17 to 12.20 g/ml (5).…”
mentioning
confidence: 83%
“…The "fl ask model" was adapted by Scaglione et al ( 1993 ), who used immune cells and human serum. This " immune model " shall simulate the bacterial timekilling behavior at the target side under drug and immune cell exposure.…”
Section: Flask Modelmentioning
confidence: 99%