A new model for chronic in vivo analysis of the periosteal microcirculation

Stuehmer, Constantin; Schumann, Paul; Bormann, Kai-Hendrik; Laschke, Matthias W.; Menger, Michael D.; Gellrich, Nils‐Claudius; Rücker, Martin

doi:10.1016/j.mvr.2008.09.008

Cited by 12 publications

(22 citation statements)

References 26 publications

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“…After soft-tissue expansion with hydrogel expander for 14 days, bone ingrowth of the augmentation material was detectable, whereas granulation tissue between the calvarial bone and the augmentation material was visible without tissue expansion (haematoxylin and eosin staining). (Stuehmer et al 2008). This chamber was successfully used in the present study and revealed the absence of inflammatory signs.…”

Section: Discussionmentioning

confidence: 59%

“…By contrast, intravital microscopy allows the perfusion of individual microvessels to be monitored even over a prolonged period of time (Menger et al 1992; Vollmar & Menger 1998). We developed and established a periosteal chamber that permits us to study periosteal microcirculation in vivo in rats (Stuehmer et al 2008). This chamber was successfully used in the present study and revealed the absence of inflammatory signs.…”

Section: Discussionmentioning

confidence: 99%

“…In Group 2, hydrogel expanders were inserted for 21 days in order to expand the soft tissue above the area to be augmented. In both groups, we used a periosteal chamber to monitor microcirculation over a period of 19 days after graft implantation (Stuehmer et al 2008). Additional eight animals were used as donors of bone tissue.…”

Section: Methodsmentioning

confidence: 99%

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Bone augmentation after soft‐tissue expansion using hydrogel expanders: effects on microcirculation and osseointegration

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Gellrich

Jachmann

et al. 2010

Clinical Oral Implants Res

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

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Bone augmentation after soft‐tissue expansion using hydrogel expanders: effects on microcirculation and osseointegration

See

Gellrich

Jachmann

et al. 2010

Clinical Oral Implants Res

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“…The statistically established difference in bone volume was concluded to be the result of bone resorption based on the reasoning outlined above. The importance of periosteal blood supply to the bone cortex as well as bone necrosis and deformities that result from periosteal deterioration indicate that resection of the periosteum in this animal model may contribute to the bone resorption that was observed . However, the reason for the difference in total bone volume in the BMP‐2 releasing constructs as compared to the empty group is unknown.…”

Section: Discussionmentioning

confidence: 95%

Tissue response to composite hydrogels for vertical bone augmentation in the rat

Kinard

Dahlin

Henslee

et al. 2013

J Biomedical Materials Res

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The objective of the present study was to develop a preclinical animal model for evaluating bone augmentation and to examine the level of bone augmentation induced by hydrogel composites. Design criteria outlined for the development of the animal model included rigid immobilization of bilateral implants apposed to the parietal bone of the rat, while avoiding the calvarial sutures. The animal model was evaluated through the implantation of hydrogel composites of oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles releasing bone morphogenetic protein-2 (BMP-2). The BMP-2 release profile was varied and compared to the implantation of a material control without BMP-2. Each hydrogel composite was implanted within a polypropylene cassette, which was immobilized to the calvarial bone using screws, and empty cassettes were implanted as a control. The design criteria for the animal model were realized; however, the level of bone augmentation did not vary between any of the groups after 4 weeks. Osteoclastic bone resorption occurred to a higher extent in groups releasing BMP-2, but the cause could not be elucidated. In conclusion, a promising bone augmentation model was established in the rat; however, refinement of the hydrogel composites was suggested to optimize the constructs for bone augmentation applications.

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“…rhodamine-6G was injected intravenously for visualizing the leukocyte adhesion. Five observation views were chosen and recorded to further determine leukocyte adhesion [38]. The leukocytes which remained stationary to postcapillary venules for 30 seconds were considered to be leukocyte adhesion [33, 39].…”

Section: Methodsmentioning

confidence: 99%

Molecular Mechanisms of Curcumin on Diabetes-Induced Endothelial Dysfunctions: Txnip, ICAM-1, and NOX2 Expressions

Wongeakin

Bhattarakosol

Patumraj

2014

BioMed Research International

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We aim to investigate the effects of curcumin on preventing diabetes-induced vascular inflammation in association with its actions on Txnip, ICAM-1, and NOX2 enzyme expressions. Male Wistar rats were divided into four groups: control (CON), diabetic (DM; streptozotocin (STZ), i.v. 55 mg/kg BW), control-treated with curcumin (CONCUR; 300 mg/kg BW), and diabetes treated with curcumin (DMCUR; 300 mg/kg BW). 12th week after STZ injection, iris blood perfusion, leukocyte adhesion, Txnip, p47phox, and malondialdehyde (MDA) levels were determined by using laser Doppler, intravital fluorescent confocal microscopy, Western Blot analysis, and TBAR assay, respectively. The iris blood perfusion of DM and DMCUR was decreased significantly compared to CON and CONCUR (P < 0.001). Plasma glucose and HbA1c of DM and DMCUR were increased significantly compared to CON and CONCUR (P < 0.001). Leukocyte adhesion, ICAM-1, p47phox expression, and MDA levels in DM were increased significantly compared to CON, CONCUR, and DMCUR (P < 0.05). Txnip expression in DM and DMCUR was significantly higher than CON and CONCUR (P < 0.05). From Pearson's analysis, the correlation between the plasma MDA level and the endothelial functions was significant. It suggested that curcumin could ameliorate diabetic vascular inflammation by decreasing ROS overproduction, reducing leukocyte-endothelium interaction, and inhibiting ICAM-1 and NOX2 expression.

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A new model for chronic in vivo analysis of the periosteal microcirculation

Cited by 12 publications

References 26 publications

Bone augmentation after soft‐tissue expansion using hydrogel expanders: effects on microcirculation and osseointegration

Bone augmentation after soft‐tissue expansion using hydrogel expanders: effects on microcirculation and osseointegration

Tissue response to composite hydrogels for vertical bone augmentation in the rat

Molecular Mechanisms of Curcumin on Diabetes-Induced Endothelial Dysfunctions: Txnip, ICAM-1, and NOX2 Expressions

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