A new model for investigating hair cell degeneration in the guinea pig following damage of the stria vascularis using a photochemical reaction

Abstract

We have established a new model for investigating the relationship between cochlear lateral wall damage and sensory cell degeneration in guinea pigs by using a photochemical reaction between the systemic injection of Rose Bengal (RB) and controlled green light irradiation to the cochlea. The photochemical reaction produced a reactive oxygen species, which then damaged the endothelium. This triggered platelet adhesion and aggregation at the site of endothelial injury to produce thrombi and affect microcirculation in the lateral wall at the site of irradiation. Changes were studied under a scanning electron microscope (SEM), and compound action potentials (CAP) were measured. SEM observations after tangential illumination of the cochlear wall revealed degeneration of the stria vascularis (SV). Specific morphological findings at 24 h included delayed degeneration of the outer hair cells concurrent with a significant increase in the CAP. Based on these findings, we suggest that degeneration of the SV was a direct result of the photochemical reaction, but CAP changes and sensory hair cell damage were secondarily caused by SV degeneration.

Estrogen inhibits apoptosis of pericytes in the cochlea stria vascularis through downregulating TMEM16A

“…Early studies have reported cochlear SV contraction in gerbils, and the contraction rate increases with age (Gratton and Schulte 1995). When the atrophy of the cochlear SV reaches a certain extent, the capillaries and PCs are reduced or lost (Ocho et al 2000), the PC cell structure is abnormal, the cytoplasm and chromatin are lost, and the cell vacuoles are increased (Neng et al 2015). Our results showed that in the 12 m group, the ABR threshold was increased, which was consistent with the process of presbycusis.…”

Recovery from cisplatin-induced ototoxicity: A case report and review

“…It has been proposed that damage to the marginal cells of the stria vascularis secondarily causes damage to the OHC [22,23]. In a study by Miyasha et al, photochemically induced damage to the cochlea with Rose Bengal dye and green light irradiation resulted in marginal cell extrusion and rupture, followed by OHC damage 12 or more hours after strial insult as seen by TEM.…”

Estrogen inhibits apoptosis of pericytes in the cochlea stria vascularis through downregulating TMEM16A

“…Early studies have reported cochlear SV contraction in gerbils, and the contraction rate increases with age (Gratton and Schulte 1995). When the atrophy of the cochlear SV reaches a certain extent, the capillaries and PCs are reduced or lost (Ocho et al 2000), the PCs cell structure is abnormal, the cytoplasm and chromatin are lost, and the cell vacuoles are abundant (Neng et al 2015). The results of our experiment showed more cochlear SV atrophy in the 12-month-old mouse group than in the 3month group.…”