A New Modular Chemiluminescence Immunoassay Analyser Evaluated

Abstract

Thyrotropin (TSH), free thyroxine (fT4) and testosterone assays have been used as a probe to evaluate the performances of a new modular chemiluminescence (CL) immunoassay analyser, the Abbott Architect 2000. The evaluation was run in parallel on other systems that use CL as the detection reaction: DPC Immulite, Chiron Diagnostics ACS-180 and ACS Centaur (TSH functional sensitivity only). TSH functional sensitivity was 0.0012, 0.009, 0.033 and 0.039 mU/I for the Architect, Immulite, ACS Centaur and ACS-180, respectively. Testosterone functional sensitivity was 0.38, 3.7 and 2.0 nmol/l for Architect, Immulite and ACS-180, respectively. Good correlation was obtained between the ACS-180 and Architect for all assays. The Immulite correlation did not agree well with the Architect or ACS-180 for fT4 and testosterone but was in good agreement for TSH. Regarding fT4 and testosterone, equilibrium dialysis and isotopic dilution gas-chromatography mass-spectrometry (GC-MS) respectively were used as reference methods. For both within- and between-run precision, the Architect showed the best reproducibility for all three analytes (CV < 6%).

Performance Characteristics of Six Third-Generation Assays for Thyroid-Stimulating Hormone

“…This assay is quite similar to the ADVIA Centaur assay that we used. A second report found functional sensitivities of 0.033 mIU/L for the ADVIA Centaur and 0.039 mIU/L for the ACS:180 when testing was conducted over a period of 6 weeks with a single lot of reagents (3 ). This report agrees well with our estimate of the functional sensitivity of the ADVIA Centaur.…”

The Biological Variation of Testosterone and Sex Hormone-Binding Globulin (SHBG) in Polycystic Ovarian Syndrome: Implications for SHBG as a Surrogate Marker of Insulin Resistance

“…There remains considerable debate about the accuracy (bias) of different total testosterone methods of measurements, especially when using automated platform analyzers such as the one used in this study. It is therefore reassuring that other studies have shown this method to compare well to reference techniques (10). Even if this were not the case, the findings of this study should not have been unduly affected, since both cases and controls used the same testosterone method.…”

“…The Architect analyzer uses chemiluminescent microparticle immunoassay technology that is based on a patented acridinium (nsulfonyl) carboxamide chemiluminescent assay. Results using this assay have been shown to demonstrate good agreement with a reference isotope dilution gas chromatography-mass spectrometry method (10). The analytical sensitivity of the insulin assay was 2 U/ml, and there was no stated cross-reactivity with proinsulin.…”

Evaluation of the UniCel™ DxI 800 Immunoassay Analyzer in Measuring Five Tumor Markers

“…The results on the linearity and imprecision of the assays by DxI were highly acceptable and in accordance with previous reports 12,14-16,21-23. In addition, we compared the results from DxI for CEA, AFP, and CA 125 with those measured by Centaur, and for CA 15-3 and CA 19-9 with those measured by ECi, as our laboratory currently uses Centaur and ECi, which were intended to be replaced with DxI, for assaying these respective tumor markers.…”