A New Monolithic-Type HPLC Column For Fast Separations

Cabrera, Karin; Lubda, Dieter; Eggenweiler, Hans-Michael; Minakuchi, H.; Nakanishi, K.

doi:10.1002/(sici)1521-4168(20000101)23:1<93::aid-jhrc93>3.3.co;2-u

Cited by 83 publications

(127 citation statements)

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“…ChiraDexbeta and ChiraDex-gamma are well-established CSPs in HPLC [26] but their use in packed CEC is investigated for the first time. A number of racemic dansyl-amino acids were separated into their enantiomers on a capillary packed with ChiraDex-beta by NA-CEC (see Table 1 and Fig.…”

Section: Resultsmentioning

confidence: 99%

Enantiomer separation by nonaqueous and aqueous capillary electrochromatography on cyclodextrin stationary phases

2001

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Native beta- and gamma-cyclodextrin bound to silica (ChiraDex-beta and ChiraDex-gamma) were packed into capillaries and used for enantiomer separation by capillary electrochromatography (CEC) under aqueous and nonaqueous conditions. Negatively charged analytes (dansyl-amino acids) were resolved into their enantiomers by nonaqueous CEC (NA-CEC). The addition of a small amount of water to the nonaqueous mobile phase enhanced the enantioselectivity but increased the elution time. The choice of the background electrolyte (BGE) determined the direction of the electroosmotic flow (EOF). With 2-(N-morpholino) ethanesulfonic acid (MES) or triethylammonium acetate (TEAA) as BGE an inverse EOF (anodic EOF) was observed while with phosphate a cathodic EOF was found. The apparent pH (pH*), the concentration of the BGE, and the nature of the mobile phase strongly influenced the elution time, the theoretical plate number and the chiral separation factor of racemic analytes.

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Section: Resultsmentioning

confidence: 99%

Enantiomer separation by nonaqueous and aqueous capillary electrochromatography on cyclodextrin stationary phases

2001

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“…Experimental parameters on conventional particulate and monolithic columns have been intensively compared [14,47,[50][51][52][53][54][55][56][57][58][59][60][61][62][63]. It has proven that methods for small drug molecules could be easily and successfully transferred to monolithic columns without the need of any modification in separation conditions [64].…”

Section: Monolithic Versus Conventional Columnsmentioning

confidence: 99%

Monolithic Silica for Fast HPLC: Current Success and Promising Future

Deeb

2011

Chromatographia

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As an approach for fast HPLC, monolithic silica has proven to be highly effective. It is especially successful for routinely obtaining fast isocratic HPLC analyses of small drug molecules. The low cost of monolithic compared with other approaches, such as UPLC, makes it more convenient for everyday application. It is also the more developed and widely applied technique compared with superficially porous particles. It offers the possibility for gaining high plate numbers through column coupling, but not at the expense of run time if a proper flow program is subsequently applied. Good precision and batch reproducibility are now achieved with commercially available monolithic silica columns. The application of monolithic silica columns is already well developed in various fields. It invades the field of bio-analysis and proteomics. Hundreds of analytical methods have already been successfully transferred to or developed on monolithic silica columns. An updated strategy based on Snyder's method for rapid method development using monolithic column has been provided. However, more is still to be expected from monolithic silica in term of chemistry variation, application, and instrument compatibility. The future of monolithic silica is promising when considering the high demand for fast chromatographic analysis.

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“…Recently, silica rod (monolithic) columns have become available that permit operation at high flow rates [73,74]. Dear et al [75] compare the results from human liver microsome filtrates obtained with a silica rod column to those using analytical columns.…”

Section: Sample Analysis By Direct Injectionmentioning

confidence: 99%

Fast LC/MS in the analysis of small molecules

Tiller

Romanyshyn

Neue

2003

Analytical and Bioanalytical Chemistry

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Liquid chromatography-mass spectrometry (LC/MS) has become one of the most widely used analytical techniques in both qualitative and quantitative analysis of small molecules. Recently, with the increasing demand for ever-higher sample throughput, the use of faster chromatographic separations has become popular, along with other LC/MS methods that decrease analytical cycle-time. The burgeoning use of LC/MS has meant that the primary expertise of many practitioners today is not in the field of LC/MS, which has been facilitated by the ease-of-use of modern LC/MS systems. An examination of the current state of the literature, relating to "fast LC/MS", should serve well to those new to LC/MS, and should help them in the development of fast LC/MS methods that are effective in terms of both the chromatography and the utilization of the mass spectrometer. This review paper focuses on fast LC/MS analyses of small molecules that have been reported in peer-reviewed publications.

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A New Monolithic-Type HPLC Column For Fast Separations

Cited by 83 publications

References 0 publications

Enantiomer separation by nonaqueous and aqueous capillary electrochromatography on cyclodextrin stationary phases

Enantiomer separation by nonaqueous and aqueous capillary electrochromatography on cyclodextrin stationary phases

Monolithic Silica for Fast HPLC: Current Success and Promising Future

Fast LC/MS in the analysis of small molecules

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