The main goal of this study is to bring together the spinors, which have a major place in several disciplines from mathematics to physics, and Positional Adapted Frame (PAF) which is a new type frame that attracts the attention of many researchers. In accordance with this purpose, we introduce the spinor representations for the trajectories endowed with PAF in the Euclidean 3-space $\mathbb{E}^3$, and construct the spinor equations of PAF vectors. Then, we find the relations between spinor representations of PAF and Serret-Frenet frame. Also we give some results and present some geometric interpretations with respect to this relationship. Moreover, we present an illustrative numerical example in order to support the given theorems and results.