A New Mutation Affecting FRQ-Less Rhythms in the Circadian System of Neurospora crassa

Li, Sanshu; Motavaze, Kamyar; Kafes, Elizabeth; Suntharalingam, Sujiththa; Lakin-Thomas, Patricia L.

doi:10.1371/journal.pgen.1002151

Cited by 13 publications

(22 citation statements)

References 49 publications

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“…For homokaryon isolation (35), a single colony was picked either from uninucleate microconidia, which were prepared as previous description (36) or from streaked cultures. Insertions of the target genes in the transformants were verified by PCR and sequencing.…”

Section: Methodsmentioning

confidence: 99%

Eukaryotic TPP riboswitch regulation of alternative splicing involving long-distance base pairing

Breaker

2013

Nucleic Acids Research

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100

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Thiamin pyrophosphate (TPP) riboswitches are found in organisms from all three domains of life. Examples in bacteria commonly repress gene expression by terminating transcription or by blocking ribosome binding, whereas most eukaryotic TPP riboswitches are predicted to regulate gene expression by modulating RNA splicing. Given the widespread distribution of eukaryotic TPP riboswitches and the diversity of their locations in precursor messenger RNAs (pre-mRNAs), we sought to examine the mechanism of alternative splicing regulation by a fungal TPP riboswitch from Neurospora crassa, which is mostly located in a large intron separating protein-coding exons. Our data reveal that this riboswitch uses a long-distance (∼530-nt separation) base-pairing interaction to regulate alternative splicing. Specifically, a portion of the TPP-binding aptamer can form a base-paired structure with a conserved sequence element (α) located near a 5′ splice site, which greatly increases use of this 5′ splice site and promotes gene expression. Comparative sequence analyses indicate that many fungal species carry a TPP riboswitch with similar intron architecture, and therefore the homologous genes in these fungi are likely to use the same mechanism. Our findings expand the scope of genetic control mechanisms relying on long-range RNA interactions to include riboswitches.

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Section: Methodsmentioning

confidence: 99%

Eukaryotic TPP riboswitch regulation of alternative splicing involving long-distance base pairing

Breaker

2013

Nucleic Acids Research

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100

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“…Although the products of these genes have not been identified yet, they are possible candidates for components of the FLO. Very recently, a mutagenesis screen identified the UV90 mutation that probably affects a factor required for a functional FLO (92).…”

Section: Frq-less Rhythms In Neurosporamentioning

confidence: 99%

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

Gyöngyösi

Káldi

2014

Antioxidants & Redox Signaling

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Significance: Both circadian rhythm and the production of reactive oxygen species (ROS) are fundamental features of aerobic eukaryotic cells. The circadian clock enhances the fitness of organisms by enabling them to anticipate cycling changes in the surroundings. ROS generation in the cell is often altered in response to environmental changes, but oscillations in ROS levels may also reflect endogenous metabolic fluctuations governed by the circadian clock. On the other hand, an effective regulation and timing of antioxidant mechanisms may be crucial in the defense of cellular integrity. Thus, an interaction between the circadian timekeeping machinery and ROS homeostasis or signaling in both directions may be of advantage at all phylogenetic levels. Recent Advances: The Frequency-White Collar-1 and White Collar-2 oscillator (FWO) of the filamentous fungus Neurospora crassa is well characterized at the molecular level. Several members of the ROS homeostasis were found to be controlled by the circadian clock, and ROS levels display circadian rhythm in Neurospora. On the other hand, multiple data indicate that ROS affect the molecular oscillator. Critical Issues: Increasing evidence suggests the interplay between ROS homeostasis and oscillators that may be partially or fully independent of the FWO. In addition, ROS may be part of a complex cellular network synchronizing non-transcriptional oscillators with timekeeping machineries based on the classical transcription-translation feedback mechanism. Future Directions: Further investigations are needed to clarify how the different layers of the bidirectional interactions between ROS homeostasis and circadian regulation are interconnected. Antioxid. Redox Signal. 20,[3007][3008][3009][3010][3011][3012][3013][3014][3015][3016][3017][3018][3019][3020][3021][3022][3023]

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“…In a frq + background, UV90 damps the amplitude of the conidiation rhythm and also damps the FRQ protein rhythm. The response of frq + cultures to phase resetting by pulses of either light or high temperature is increased in the UV90 mutant background, consistent with a decrease in the amplitude of the circadian oscillator (Li et al ., 2011). These results suggest that the UV90 gene product is an important component of the FLO and is also required to maintain the amplitude of the FRQ/WCC oscillator, indicating an influence of the FLO on FRQ/WCC.…”

Section: Frq-less Rhythmsmentioning

confidence: 63%

“…Using the chol-1 frq 10 double mutant, a mutagenesis screen was carried out to look for genes that affect the FRQ-less rhythm on low choline (Li et al ., 2011). A new mutation was found, named UV90, that abolishes the FRQ-less conidiation rhythm seen in low-choline cultures.…”

Section: Frq-less Rhythmsmentioning

confidence: 99%

The Genetics of Circadian Rhythms in Neurospora

Lakin-Thomas¹,

Bell-Pedersen²,

Brody³

2011

The Genetics of Circadian Rhythms

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This chapter describes our current understanding of the genetics of the Neurospora clock and summarizes the important findings in this area in the past decade. Neurospora is the most intensively studied clock system, and the reasons for this are listed. A discussion of the genetic interactions between clock mutants is included, highlighting the utility of dissecting complex mechanisms by genetic means. The molecular details of the Neurospora circadian clock mechanism are described, as well as the mutations that affect the key clock proteins, FRQ, WC-1, and WC-2, with an emphasis on the roles of protein phosphorylation. Studies on additional genes affecting clock properties are described and place these genes into two categories: those that affect the FRQ/WCC oscillator and those that do not. A discussion of temperature compensation and the mutants affecting this property is included. A section is devoted to the observations pertinent to the existence of other oscillators in this organism with respect to their properties, their effects, and their preliminary characterization. The output of the clock and the control of clock-controlled genes are discussed, emphasizing the phasing of these genes and the layers of control. In conclusion, the authors provide an outlook summarizing their suggestions for areas that would be fruitful for further exploration.

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A New Mutation Affecting FRQ-Less Rhythms in the Circadian System of Neurospora crassa

Cited by 13 publications

References 49 publications

Eukaryotic TPP riboswitch regulation of alternative splicing involving long-distance base pairing

Eukaryotic TPP riboswitch regulation of alternative splicing involving long-distance base pairing

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

The Genetics of Circadian Rhythms in Neurospora

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