A new ophthalmic formulation containing antiseptics and dexpanthenol: In vitro antimicrobial activity and effects on corneal and conjunctival epithelial cells

Mencucci, Rita; Favuzza, Eleonora; Bottino, Paolo; Mazzantini, Costanza; Zanotto, Elisa; Pellegrini‐Giampietro, Domenico E.; Landucci, Elisa

doi:10.1016/j.exer.2020.108269

Cited by 19 publications

(17 citation statements)

References 33 publications

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“…Human corneal epithelial cells (HCE-2) were cultured in keratinocyte medium that was changed twice a week and were incubated at 37 °C in an atmosphere of humidified air and 5% CO 2 , in precoated flasks [ 6 ].…”

Section: Methodsmentioning

confidence: 99%

“…Human conjunctival epithelial cells (HConEC) were cultured in conjunctival epithelial cell medium, plated in flasks previously treated with poly- l -lysine, in an atmosphere of humidified air and 5% CO 2 at 37 °C as previously reported [ 6 ].…”

Section: Methodsmentioning

confidence: 99%

“…Eye infections, and especially endophthalmitis, can have a devastating effect on the patient's visual function [1]. As a result of the increase in antibiotic resistance in ocular bacterial strains caused by the excessive use of antibiotics in the treatment and prophylaxis of ocular infections [2], interest in antiseptics in ophthalmology is growing [3][4][5][6]. Nevertheless, antiseptics can be toxic for the ocular surface, causing corneal epithelial defects or epithelial damage, especially at high concentrations [7].…”

Section: Introductionmentioning

confidence: 99%

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Antiseptics and the Ocular Surface: In Vitro Antimicrobial Activity and Effects on Conjunctival and Corneal Epithelial Cells of a New Liposomal Ocular Spray Containing Biosecur® Citrus Extract

et al. 2022

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Introduction:The study aimed to evaluate the in vitro antimicrobial activity of a new liposomal ocular spray containing the antiseptic Biosecur Ò citrus extract (Oftasecur, OFF-HEALTH, Florence, Italy) and its in vitro effects on cultured human corneal and conjunctival cells. Methods: Minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) of Oftasecur against Candida albicans and Gram-positive and Gram-negative bacteria, including antibiotic-resistant strains, were determined. Human corneal and conjunctival epithelial cells in vitro were incubated for 10 and 30 min with Oftasecur or its components. The cytotoxicity was assessed through the release of cytoplasmic enzyme lactate dehydrogenase (LDH) into the medium; the 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate the cell viability. Results: Oftasecur was active at dilutions ranging from 1:2 to 1:16 and it displayed bactericidal and fungicidal effect against all assayed microorganisms. Most of the reduction of Staphylococcus epidermidis vitality (65%) occurred within the first minute of exposure. The cytotoxicity of Oftasecur was similar to its vehicle, and the cell viability was significantly reduced only by Oftasecur in its undiluted form. Conversely, Biosecur induced a significant cytotoxicity in all the experiments. Conclusion: Oftasecur showed a rapid and wide-spectrum antibacterial activity, with an optimal in vitro tolerability profile.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antiseptics and the Ocular Surface: In Vitro Antimicrobial Activity and Effects on Conjunctival and Corneal Epithelial Cells of a New Liposomal Ocular Spray Containing Biosecur® Citrus Extract

et al. 2022

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“…Damage in human corneal and conjunctival epithelial cells was quantitatively assessed by measuring the amount of LDH released by the damaged cells into the extracellular fluid, 15 min and 1 h after drug exposure, using the LDH kit, as previously described [26]. The LDH level corresponding to complete cell death was determined for each experiment by analyzing sister cultures exposed to 0.01% BAK.…”

Section: Ldh Assaymentioning

confidence: 99%

Preparation of Liposomal Formulations for Ocular Delivery of Thymoquinone: In Vitro Evaluation in HCEC-2 e HConEC Cells

et al. 2021

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Thymoquinone (TQ) is the main constituent of Nigella sativa L. essential oil. In vitro studies have shown its protective effect against H2O2-induced oxidative stress in human retinal pigment epithelium cells, and in vivo experiments have demonstrated its effect in decreasing corneal neovascularization and reducing the inflammation in an experimental dry eye model in mice. Its therapeutic use is limited by poor bioavailability, low solubility, and scarce permeability. In this study, two liposomal formulations have been developed, both of which consist of phosphatidylcholine and Plurol Oleique, a liquid lipid, and one of which is coated with 0.1% w/v hyaluronic acid (HA) to increase both TQ solubility and its ocular therapeutic potential. Each formulation has a size <200 nm and an EE% around 70%, determined by scattering techniques and the HPLC-DAD analytical method, respectively, and they result in a 2-fold increase in TQ solubility. HA-coated liposomes are stable over 2 months at +4 °C, and coated and uncoated liposomes present a gradual and prolonged release of TQ. Two cell lines, human corneal epithelial cells (HCEC-2) and human conjunctival epithelial cells (HConEC) were used to investigate the safety of the liposomal formulations. Uptake studies were also performed using fluorescent liposomes. Both liposomes and, in particular, HA-coated liposomes reduce the TQ toxicity observed at high doses in both HCEC-2 and HConEC cells, and both formulations increase the absorption at the cellular level and especially at the nucleus level, with a more pronounced effect for HA-coated liposomes.

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“…It has a well-known effect in promoting epithelial healing. The effects on the OS were recently confirmed by Mencucci et al in an in vitro study of human conjunctival and corneal epithelial cells treated for 18 h with a solution containing dexpanthenol 5% and polyvinyl alcohol [37]. Additionally, the role of vitamin B12 in the maintenance of a correct nerve trophism has been largely demonstrated in preclinical studies [2].…”

Section: Vitamin B 321 Preclinical Evidencementioning

confidence: 75%

A Review of Topical and Systemic Vitamin Supplementation in Ocular Surface Diseases

et al. 2021

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In the homeostasis of the ocular surface, vitamins play a critical role in regulating inflammatory responses and promoting cell differentiation, development and correct function. Systemic vitamin supplementation has been available for many decades; in recent years, thanks to pharmacological advancements, topical vitamin delivery has also become available in an attempt to better treat ocular surface disease (OSD) and dry eye disease (DED). In this paper, we reviewed the current evidence on the role of vitamin supplementation in OSD and DED. We originally searched the PubMed archive, inspected the references and restricted the search to pertinent papers. The body of evidence was evaluated using the amelioration of both signs and symptoms as the outcome, when available. We found that in patients with vitamin deficiency, systemic supplementation of Vitamin A is effective in treating OSD, reducing both DED signs and symptoms. Additionally, systemic supplementation of vitamin D is useful in reducing DED symptoms and increasing tear volume. Vitamin A is also effective in reducing DED signs and symptoms when administered locally. The efficacy of supplementation with other vitamins is still not fully proven. In conclusion, the inclusion of vitamins into the treatment strategies for OSD and DED allows for better treatment customization and better outcomes in these patients.

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A new ophthalmic formulation containing antiseptics and dexpanthenol: In vitro antimicrobial activity and effects on corneal and conjunctival epithelial cells

Cited by 19 publications

References 33 publications

Antiseptics and the Ocular Surface: In Vitro Antimicrobial Activity and Effects on Conjunctival and Corneal Epithelial Cells of a New Liposomal Ocular Spray Containing Biosecur® Citrus Extract

Antiseptics and the Ocular Surface: In Vitro Antimicrobial Activity and Effects on Conjunctival and Corneal Epithelial Cells of a New Liposomal Ocular Spray Containing Biosecur® Citrus Extract

Preparation of Liposomal Formulations for Ocular Delivery of Thymoquinone: In Vitro Evaluation in HCEC-2 e HConEC Cells

A Review of Topical and Systemic Vitamin Supplementation in Ocular Surface Diseases

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