A new organic phase enzyme electrode for the analysis of organophosphorus pesticides and carbamates

Campanella, L.; Luca, S. De; Sammartino, Maria Pia; Tomassetti, M.

doi:10.1016/s0003-2670(98)00806-x

Cited by 58 publications

(23 citation statements)

References 32 publications

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“…According to Mionetto et al, the activity of immobilized AChE remained in hydrophobic solvents like hydrocarbons but decreased in polar solvents such as alcohols. Similar results were obtained by Campanella et al [25]. They developed a bienzymatic amperometric oxygenelectrode with immobilized butyrylcholinesterase and choline oxidase for the detection of organophosphates and carbamates working in chloroform / n-hexane mixtures.…”

Section: Introductionsupporting

confidence: 84%

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Schulze

Schmid

Bachmann

2002

Analytical and Bioanalytical Chemistry

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The extensive use of pesticides to protect agricultural crops necessitates reliable tools for the detection of residues in food and water, thus ensuring environmental protection and consumer safety. Neuroinhibitors such as organophosphates and carbamates in particular, represent a potential hazard to human health. These compounds are frequently found in food but conventional methods of analysis are limited as they are either time consuming or not sufficiently sensitive. As a result, a rapid and sensitive biosensor test based on AChE-inhibition was developed. The disposable AChE-biosensor was directly applied in solvent extracts of food samples using isooctane as extraction solvent. A complete assay could be performed in less than 2 hours. Recovery rates of 84 % were obtained in tests with spiked orange juice samples. Tests in food samples with a lower water content resulted in reduced recovery rates (44 % for peach pap baby food). Phosphorothionate insecticides could be detected after direct oxidation in food with N-bromosuccinimide and solvent extraction. The assay displayed a detection limit of 2 µg/kg paraoxon which was sufficient for the monitoring of maximum residue limits in food according to EU regulations.3

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Section: Introductionsupporting

confidence: 84%

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Schulze

Schmid

Bachmann

2002

Analytical and Bioanalytical Chemistry

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“…A different method was chosen by Campanella et al, demonstrating the suitability of a bi-enzymatic biosensor (BChE + ChOx) to work in the organic phase (chloroformn-hexane 1:1 mixture) [166].…”

Section: Measurement Of Insecticides and Nerve Agents In Presence Of mentioning

confidence: 99%

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

et al. 2010

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The present review reports the research carried out during last 9 years on biosensors based on cholinesterase inhibition for nerve agents, organophosphorus and carbammic insecticides, and aflatoxin B 1 detection. Relative applications in environmental and food areas are also reported. Special attention is paid to the optimization of parameters such as enzyme immobilization, substrate concentration, and incubation time in the case of reversible inhibition by aflatoxin B 1 or irreversible inhibition by organophosphorus and carbamic insecticides, and nerve agents in order to optimize and improve the analytical performances of the biosensor. Evaluation of selectivity of the system is also discussed.

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“…Although these parameters are deeply affected by various factors such as biosensors fabrication method, experimental conditions and type of organophosphorous material, nonetheless, the analytical parameters of the proposed mono-enzyme biosensor favorably compare with most of the data reported in the literature. For instance, in the case of incubation time, La Rosa [25] used an incubation time of 3-5 min for paraoxon and carbaryl detection while Bernabei and Campanella used 30 and 20 min, respectively [26,27] for aldicarb and paraoxon detection. Nunes used 10 min incubation time for aldicarb, and an incubation time as high as 1 h was reported by Skladal and Cho [28,29].…”

Section: Resultsmentioning

confidence: 99%

Mono-Enzyme Biosensor for the Detection of Organophosphorous Compounds

Tavakoli

Ghourchian

2010

JICS

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Organophosphorous compounds are important neuroactive molecules whose presents significant analytical challenges. New monoenzyme biosensor introduced here, is used for the measurement of the level of two organophosphorous compounds, paraoxon (POX) and ethyl parathion (EPA), in phosphate buffer. Prussian blue electrodepositionized on a graphite electrode served as a template for immobilization of monoenzyme layer. A monoenzyme layer containing choline oxidase was immobilized along with nafion and bovine serum albumin, by cross-linking with glutaraldehyde. The results revealed that the response of the proposed biosensor was linear between 5 μM and 80 μM in the presence of POX and EPA. Detection limits were 0.4 μM in both cases. Also, for the optimization of the biosensor performance, temperature and pH dependency, concentration of choline chloride as substrate of choline oxidase and incubation time were determined. The results showed that the optimum values for temperature, pH, substrate concentration and incubation time were room temperature, pH 7, 4 mM and 30 min, respectively.

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A new organic phase enzyme electrode for the analysis of organophosphorus pesticides and carbamates

Cited by 58 publications

References 32 publications

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

Mono-Enzyme Biosensor for the Detection of Organophosphorous Compounds

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