A new ovine Babesia species transmitted by Hyalomma anatolicum anatolicum

Guan, Guiquan; Ma, Miling; Moreau, Emmanuelle; Liu, Junlong; Lu, Bingyi; Bai, Quan; Luo, Jianxun; Jorgensen, W.K.; Chauvin, Alain; Yin, Hong

doi:10.1016/j.exppara.2009.05.001

“…Blast analysis of these sequences revealed highest identities with Babesia sp. Xinjiang [GenBank: DQ159073] (98.94 to 99.40% depending on the red deer sequence type) with 5 to 8 nucleotide changes (Table 2) [25]. Close identities with Babesia sp.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of Babesia pecorum sp. nov. from farmed red deer (Cervus elaphus)

Jouglin

¹

,

Fernández-de-Mera

²

,

Cotte

³

et al. 2014

Vet Res

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The diversity of Babesia species infecting cervids in parts of central and southern Spain was analyzed by collecting blood from farmed red deer (Cervus elaphus). Babesia sp. was isolated in vitro from two red deer herds in Cádiz and Ciudad Real. The number of Babesia sp. carriers differed between the two herds: 36/77 in Cádiz and 1/35 in Ciudad Real. Hyalomma lusitanicum was the most prevalent tick species identified on the Cádiz farm vegetation and on sampled animals, and is therefore a candidate vector. The molecular characteristics of 21 isolates were determined by complete (8 isolates) or partial (13 isolates) 18S rRNA gene sequencing. The sequences were highly similar (over 99.4% identity) and 6 sequence types were identified at the level of one herd only, demonstrating a rather high genetic diversity. They formed a monophyletic clade, and members of the three main sequence types shared a similar morphology and the same erythrocyte susceptibility pattern. This clade also included Babesia sp. Xinjiang isolated from sheep in China and Babesia sp. identified in giraffe in South Africa, with identities higher than 98.3% and statistically relevant phylogenetic support. None of the biological properties analyzed for both Babesia from red deer and Babesia sp. Xinjiang allowed their differentiation (ability to develop in vitro in erythrocytes from cattle and sheep, as well as in erythrocytes from different cervids, unsuccessful infection of calves). We propose the Babesia isolated from red deer as a new species named B. pecorum. Whether Babesia sp. Xinjiang and the Babesia characterized in South Africa belong to the same species is debated.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-014-0078-7) contains supplementary material, which is available to authorized users.

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“…Members of the monophyletic group which includes Babesia sp. Xinjiang from China [25], and the Babesia from South African ruminants [26] could be added later as members of this newly described species, since this infraorder groups their host families i.e. bovidae, giraffidae and cervidae.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of

Jouglin

¹

,

Fernández-de-Mera

²

,

Cotte

³

et al. 2014

Vet Res

Self Cite

View full text Add to dashboard Cite

The diversity of Babesia species infecting cervids in parts of central and southern Spain was analyzed by collecting blood from farmed red deer (Cervus elaphus). Babesia sp. was isolated in vitro from two red deer herds in Cádiz and Ciudad Real. The number of Babesia sp. carriers differed between the two herds: 36/77 in Cádiz and 1/35 in Ciudad Real. Hyalomma lusitanicum was the most prevalent tick species identified on the Cádiz farm vegetation and on sampled animals, and is therefore a candidate vector. The molecular characteristics of 21 isolates were determined by complete (8 isolates) or partial (13 isolates) 18S rRNA gene sequencing. The sequences were highly similar (over 99.4% identity) and 6 sequence types were identified at the level of one herd only, demonstrating a rather high genetic diversity. They formed a monophyletic clade, and members of the three main sequence types shared a similar morphology and the same erythrocyte susceptibility pattern. This clade also included Babesia sp. Xinjiang isolated from sheep in China and Babesia sp. identified in giraffe in South Africa, with identities higher than 98.3% and statistically relevant phylogenetic support. None of the biological properties analyzed for both Babesia from red deer and Babesia sp. Xinjiang allowed their differentiation (ability to develop in vitro in erythrocytes from cattle and sheep, as well as in erythrocytes from different cervids, unsuccessful infection of calves). We propose the Babesia isolated from red deer as a new species named B. pecorum. Whether Babesia sp. Xinjiang and the Babesia characterized in South Africa belong to the same species is debated.

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“…Several species were described in small ruminants: B. ovis, B. motasi, Babesia foliata, Babesia taylori and B. crassa, and the recently identified Babesia (Lintan) (Guan et al, 2008;Ranjbar-Bahadori et al, 2012). Ovine babesiosis is prevalent in Eastern Asia, Iran, the Mediterranean basin and North Africa (Guan et al, 2009;Liu et al, 2007;Ranjbar-Bahadori et al, 2012;Rjeibi et al, 2014;Sevinc et al, 2013;Uilenberg, 2006;Yeruham et al, 1985). Clinical symptoms of the disease include fever, anemia, loss of appetite, hemoglobinuria, icterus, and death (Yeruham et al, 1998b).…”

Section: Introductionmentioning

confidence: 94%