A new pancreatic lipase inhibitor isolated from the roots of Actinidia arguta

Abstract

A new coumaroyl triterpene, 3-O-trans-p-coumaroyl actinidic acid (1), as well as five known triterpenes, ursolic acid (2), 23-hydroxyursolic acid (3), corosolic acid (4), asiatic acid (5) and betulinic acid (6) were isolated from an EtOAc-soluble extract of the roots of Actinidia arguta. The structure of compound 1 was elucidated from interpretation of the spectroscopic data, particularly by extensive 1D and 2D NMR studies. All the isolates (1-6) were evaluated in vitro for their inhibitory activities on pancreatic lipase (PL). Of the isolates, the new compound 1 possessed the highest inhibitory activity on PL, with an IC(50) of 14.95 microM, followed by ursolic acid (2, IC(50) = 15.83 microM). The other four triterpenes (3-6) also showed significant PL inhibitory activity, with IC(50) values ranging from 20.42 to 76.45 microM.

Anti-lipase and lipolytic activities of ursolic acid isolated from the roots of Actinidia arguta

“…We previously reported that ursolic acid was isolated from the roots of A. arguta (Jang et al, 2008).…”

“…There are a number of reports showing that ursolic acid exerts various biological activities including anti-tumor, anti-inflammatory, anti-microbial, anti-fungal and anti-hyperlipidemic properties (Huang et al, 1994;Lim et al, 2007;Manez et al, 1997;Min et al, 2008;Najid et al, 1992;Ohigashi et al, 1986). In a previous study, ursolic acid isolated from the roots of A. arguta, was shown to exert pancreatic lipase inhibition in vitro (Jang et al, 2008). Based on the inhibitory activity of ursolic acid on pancreatic lipase in vitro, we tested whether ursolic acid could inhibit fat absorption in vivo.…”

“…Previous phytochemical investigations on A. arguta resulted in the isolation of flavonoids (Webby and Markham, 1990), phenolic compounds (Lim et al, 2006), triterpenes (Whang et al, 2000) and lignans (Whang et al, 2000). Recently, a new pentacyclic triterpene, 3-O-trans-pcoumaroyl actinidic acid, was isolated from the roots of A. arguta by our group (Jang et al, 2008).…”

Effect of Peucedanum japonicum Thunb Extract on High-fat Diet-induced Obesity and Gene Expression in Mice

“…The anti-obesity activities of several natural compounds have been ascribed to increased fecal fat excretion via the inhibition of lipase activity in previous studies 20,21 . The present study, therefore, compared the lipase inhibition activities of the extracts, as shown in Fig.…”

Inhibition of growth and biofilm formation of Staphylococcus aureus by corosolic acid

“…Corosolic acid는 pentacyclic triterpenoid 형태로서 바나바 나뭇 잎(Lagerstroemia speciosa L.), 다래나무(Actinidia arguta)잎, 아몬 드(Prunus dulcis) 등으로부터 추출되어 부작용 없이 비만, 당뇨 병, 고지혈증 및 종양 치료에 효과적이라고 보고된 바 있다 (8)(9)(10)(11)(12) Table 3).…”