A New Peptide Nucleic Acid Fluorescence <i>In Situ</i> Hybridization Probe for the Specific Detection of <i>Salmonella</i> Species in Food Matrices

Sousa, Mário; Rocha, Rui; Araújo, Daniela; Castro, Joana; Barbosa, Ana; Azevedo, Nuno F.; Cerqueira, Laura; Almeida, Carina

doi:10.1089/fpd.2023.0127

Foodborne Pathogens and Disease

2024

DOI: 10.1089/fpd.2023.0127

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A New Peptide Nucleic Acid Fluorescence In Situ Hybridization Probe for the Specific Detection of Salmonella Species in Food Matrices

Mário Sousa,

Rui Rocha,

Daniela Araújo

et al.

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2024

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Cited by 2 publications

References 28 publications

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Active Quantum Biomaterials‐Enhanced Microrobots for Food Safety

Jyoti,

Castillo,

Jurado‐Sánchez

et al. 2024

Small

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Timely disruptive tools for the detection of pathogens in foods are needed to face global health and economic challenges. Herein, the utilization of quantum biomaterials‐enhanced microrobots (QBEMRs) as autonomous mobile sensors designed for the precise detection of endotoxins originating from Salmonella enterica (S. enterica) as an indicator species for food‐borne contamination globally is presented. A fluorescent molecule‐labeled affinity peptide functions as a specific probe, is quenched upon binding to the surface of QBEMRs. Owing to its selective affinity for endotoxin, in the presence of S. enterica the fluorescence is restored and easy to observe and quantifies optical color change to indicate the presence of Salmonella. The devised approach is designed to achieve highly sensitive detection of the S. enterica serovar Typhimurium endotoxin with exquisite selectivity through the utilization of QBEMRs. Notably, no fluorescence signal is observed in the presence of endotoxins bearing similar structural characteristics, highlighting the selectivity of the approach during food sample analysis. Technically, the strategy is implemented in microplate readers to extend microrobots‐based approaches to the routine laboratory. This new platform can provide fast and anticipated results in food safety.

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Active Quantum Biomaterials‐Enhanced Microrobots for Food Safety

Jyoti,

Castillo,

Jurado‐Sánchez

et al. 2024

Small

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Evaluation of Simultaneous Growth of Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes in Ground Beef Samples in Different Growth Media

Sousa,

Barbosa,

Araújo

et al. 2024

Foods

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Several multiplex approaches for the simultaneous detection of pathogens in food have been developed in recent years, but the use of a single enrichment medium remains a problem. In this study, six enrichment broths (five non-selective media, tryptic soy broth (TSB), brain heart infusion broth (BHI), buffered peptone water (BPW), universal pre-enrichment broth (UPB), no. 17 broth, and a selective, Salmonella Escherichia Listeria broth (SEL)), were studied for the simultaneous detection of E. coli O157:H7, Salmonella spp., and L. monocytogenes, to validate the suitable enrichment broth to be used for the detection methods. Different ratios of E. coli O157:H7, Salmonella spp., and L. monocytogenes were used. Almost all non-selective broths evaluated in this study showed similar growth parameters and profiles among each other. The only selective enrichment broth under analysis (SEL) showed distinct growth features compared to the non-selective media, allowing for a slower but balanced growth of the three pathogens, which could be beneficial in preventing the overgrowth of fast-growing bacteria. In addition, when tested in ground beef samples, SEL broth seems to be the most distinctive medium with a balanced growth pattern observed for the three pathogens. Overall, this study is intended to provide the basis for the selection of suitable enrichment broths according to the technology detection to be used, the desired time of enrichment, and the expected balanced concentration of pathogens.

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A New Peptide Nucleic Acid Fluorescence In Situ Hybridization Probe for the Specific Detection of Salmonella Species in Food Matrices

Cited by 2 publications

References 28 publications

Active Quantum Biomaterials‐Enhanced Microrobots for Food Safety

Active Quantum Biomaterials‐Enhanced Microrobots for Food Safety

Evaluation of Simultaneous Growth of Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes in Ground Beef Samples in Different Growth Media

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