A new point-of-care test for the rapid detection of urinary tract infections

Abstract

Urinary tract infections (UTIs) are among the most common infections in all age groups. Fast and accurate diagnosis is essential to ensure a timely and effective therapy. Alongside with reference culture-based methods, several point-of-care tests (POCTs) for early detection of UTIs have been developed, but they have not been significantly implemented in current clinical practice. The Micro Biological Survey (MBS) POCT is a simple test developed by MBS Diagnostics Ltd. (London, UK) for the detection and management of UTIs. The present study has been undertaken to investigate the potentials and limits of the MBS POCT. A total of 349 patients were enrolled in two open-label, monocentric, non-interventional clinical trials in collaboration with an Emergency Medicine department and the outpatient clinic of two hospitals in Rome. Results of urine analysis using the MBS POCT were compared with those of the routine culture-based tests for UTI diagnosis performed by the hospital laboratory. The MBS POCT provided fast results revealing high bacterial count UTIs (≥ 10 5 CFU/ml) with 97% accuracy, 92% sensitivity, 100% specificity, 99% PPV, and 96% NPV within a 5-h analytical time threshold.

Validación de un dispositivo point-of-care para la detección rápida de infección urinaria y susceptibilidad antimicrobiana

“…Además, estos dos últimos necesitan un mínimo de 12 h de incubación para su posterior interpretación. En con-traposición, un nuevo dispositivo, que también utiliza un indicador redox para detectar el crecimiento bacteriano y tecnologías basadas en biosensores funcionalizados con un panel de sondas de ADN, permiten diagnosticar ITU con sensibilidades de 91,9 y 98,5% y especificidades de 99,5 y 96,6%, respectivamente 15,16 . Aunque estos valores son más altos que los obtenidos para el dispositivo KAR ® , estas metodologías conllevan un coste económico más elevado y no se podrían implementar en zonas con bajos recursos.…”

Anthocran® Phytosome®: Prevention of Recurring Urinary Infections and Symptoms after Catheterization

“…frequency, dysuria, nocturia, urgency, pain), presence of increased bacterial charge in the urines, visible presence of blood in the urine and need for consultation and specialist's evaluation. Clinical efficacy of the supplement ingredient was determined by evaluating main target parameters: signs/symptoms (on a visual analogue scale ranging from 0 to 4), the presence of blood cells in the urine and bacterial contamination (as in Table 1) (30,31) and recurrence of a UTI. Safety and tolerability were also assessed by weekly contacts and laboratory measurements.…”

Smart Diaper Based on Integrated Multiplex Carbon Nanotube-Coated Electrode Array Sensors for In Situ Urine Monitoring

“…It is thus necessary to develop a rapid, easy-to-perform urine screening method to determine metabolic indicators for assessing the health status of infants, children, bedside patients, seniors, and other patients with urinary incontinence. , Point-of-care testing (POCT) meets the needs for a more timely diagnosis and rapid clinical decision making for targeted therapy, with urine test strips being the prominent example of a commercial POCT tool for rapid detection of urinary tract infections. , However, commercial diapers or urine test strips are methods based on optical detection of color changes but cannot provide real-time monitoring or recording from mobile devices. Optical detection is also limited to describing low-concentration indicators due to their low detection accuracy and sensitivity. , …”