A New Polymorphism of Nucleolar Organizer Regions (NORs) of Indian Muntjac (Muntiacus muntjak) in Laos PDR

Abstract

Summary This work is the first description of a cytogenetic study of the Indian muntjac (Muntiacus muntjak) in Laos PDR. Blood samples were taken from 1 male and 1 female Indian muntjac kept in Laos Zoo, Laos PDR. Lymphocytes were cultured following standard methods. Cell harvesting was performed and followed by metaphase chromosome spreading on microscopic slides. Conventional staining, G-banding and high-resolution techniques were then applied using Giemsa's stain. The results showed that the number of diploid chromosomes (2n) of the Indian muntjac was 6 in female and 7 in male. The short arm of chromosome pair 3 referred to the X chromosome. Idiograms represent the banding pattern at the metaphase chromosome and prometaphase chromosome were 149 and 192 bands, respectively. The polymorphism of nucleolar organizer regions (NORs) was found at 2 sites on chromosome pair 1, and 1 site on chromosome pair 3 (heteromorphic, 3a3b) in the female, whereas they were found at 2 sites on chromosome pair 1 and 2 sites on chromosome pair 3 (homomorphic, 3a3a) in the male. These results will be useful for providing basic genetic information and future studies of the breeding, conservation and chromosome evolution in this animal.

Karyotype and Idiogram of the Axis Deer (<i>Axia axis</i>, Cervidae) by Conventional Staining, GTG-, High-Resolution GTG-, and Ag-NOR-Banding Techniques

“…The extant deer species have diverse karyotypes; their diploid chromosome numbers range from 2n= 6 in the female Indian muntjac (Muntiacus muntjak vaginalis) (Wurster and Benirschke 1970) to 2n=80 in the Capreolus capreolus pygargus, which make them an ideal group for chromosomal evolution studies. It is proposed that the ancestral cervid karyotype is 2n=70 based on comparative karyotype analysis of many deer species (Neitzel 1987, Tanomtong et al 2005, 2010, Huang et al 2005.…”

Karyotype and Idiogram of Indian Hog Deer (<i>Hyelaphus porcinus</i>) by Conventional Staining, GTG-, High-Resolution and Ag-NOR Banding Techniques

“…The extant deer species have diverse karyotypes; their diploid chromosome numbers range from 2n= 6 in the female Indian muntjac (Muntiacus muntjak vaginalis) to 2n=80 in the Siberian roe deer (Capreolus capreolus pygargus) (Wurster and Benirschke 1970, Neitzel 1987, Tanomtong et al 2005, Huang et al 2005, Tanomtong et al 2010, Khongcharoensuk et al 2017.…”