A new potent natural antioxidant mixture provides global protection against oxidative skin cell damage

Abstract

Oxidative stress occurs when there is an over production of free radicals and cells are not able to neutralize them by their own antioxidant mechanisms. These excess of free radicals will attack cellular macromolecules leading to cell damage, function impairment or death. Because of that, antioxidant substances have been largely used in products to offer complementary protection. In this study a new mixture of three known antioxidants (cocoa, green tea and alpha-tocopherol) was evaluated and its antioxidant protection was assessed focusing on its capacity to protect main cell macromolecules. Results have shown that it has a high antioxidant capacity by protecting lipids, DNA and proteins against oxidative damage. The antioxidant effect of the mixture on cells was also investigated and it was able to reduce oxidative stress generated by lipopolisacharide in human fibroblasts. Finally, as the mixture has proved to be highly antioxidant, its effect on cell senescence was evaluated, and it was demonstrated that fibroblasts in culture had delayed senescence when treated with these actives on a mixture. All results together provide important data about a new antioxidant mixture that uses a small amount of actives and is able to protect cell against oxidative damages in a global way.

Anti-Ageing Effects of Sonchus oleraceus L. (pūhā) Leaf Extracts on H2O2-Induced Cell Senescence

“…This suggested that S. oleraceus extracts can not only assist cells to recover from acute H 2 O 2 stress, but can also protect cells from chronic stress applied for five consecutive days as tested in the present study. Similar to the present results, a mixture of cocoa and green tea extracts, rich in polyphenols, and vitamin E significantly reduced UV stress-induced premature senescence from 51.67% to 38.81% in human dermal fibroblasts [46]. Oxidative DNA damage has been demonstrated to be proportional to the age of WI-38 human fibroblast cells [9] and was proposed to be another biomarker for cellular senescence.…”

Eckol Inhibits Particulate Matter 2.5-Induced Skin Keratinocyte Damage via MAPK Signaling Pathway

“…To explore the mechanism in detail, we checked the state of intracellular molecules such as lipids, protein, and DNA, which play various important roles in the cells [31]. Furthermore, intracellular macromolecular damage can be recognized as oxidative stress [32].…”

Functional Properties of Ascidians By-products: Nutritional and Medicinal Value

“…Therefore, antioxidant-rich sources and supplements are under the spotlight to offer extra protection. Although many chemical substances have been proved for their antioxidant activity, there has been a continuous search for new antioxidants from natural sources because of the poor bioavailability, cumulative effects inside the body, and adverse side effects of synthetic substances (Jorge et al 2011 ). Many researches among ascidians tunics showed their strong antioxidant effects and they have similar or higher defense activity as the raw contents of ascidians (Table 27. 6 , Jo et al 2010 ;Kim et al 2005 ;Nacional et al 2011 ;Park et al 2010 ).…”