A new preservation solution for lung transplantation: Evaluation in a porcine transplantation model

Pizanis, Nikolaus; Petrov, Andrey; Heckmann, Jens; Wiswedel, Ingrid; Wohlschläger, J.; Groot, Herbert de; Jakob, Heinz; Rauen, Ursula; Kamler, Markus

doi:10.1016/j.healun.2011.11.009

Cited by 27 publications

(33 citation statements)

References 19 publications

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“…With partial substitution of histidine with N-α-acetyl-L-histidine and the complement of iron chelators and AAs, HTK-N was expected to be a promising alternative to the currently used standard preservation solutions UW and HTK. Previous studies documented nontoxic property of HTK-N in a variety of organs and grafts including liver, kidney, lung, pancreas, intestine and heart [51][52][53][54][55][56][57]. In addition, animal models showed protective effects of HTK-N on different organs including liver [18,51,52,58], lung [53] and heart [54,59,60].…”

Section: Discussionmentioning

confidence: 94%

“…Previous studies documented nontoxic property of HTK-N in a variety of organs and grafts including liver, kidney, lung, pancreas, intestine and heart [51][52][53][54][55][56][57]. In addition, animal models showed protective effects of HTK-N on different organs including liver [18,51,52,58], lung [53] and heart [54,59,60]. On the contrary, no noticeable superiority of HTK-N to HTK was found in studies on kidney [55], pancreas [56] and vascularized tissue isograft [61].…”

Section: Discussionmentioning

confidence: 96%

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A novel histidine–tryptophan–ketoglutarate formulation ameliorates intestinal injury in a cold storage and ex vivo warm oxygenated reperfusion model in rats

et al. 2020

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Correspondence: Linus Kebschull (Kebschull@googlemail.com)Aim: The present study aims to evaluate protective effects of a novel histidine-tryptophan-k etoglutarate solution (HTK-N) and to investigate positive impacts of an additional luminal preservation route in cold storage-induced injury on rat small bowels. Methods: Male Lewis rats were utilized as donors of small bowel grafts. Vascular or vascular plus luminal preservation were conducted with HTK or HTK-N and grafts were stored at 4 • C for 8 h followed by ex vivo warm oxygenated reperfusion with Krebs-Henseleit buffer for 30 min. Afterwards, intestinal tissue and portal vein effluent samples were collected for evaluation of morphological alterations, mucosal permeability and graft vitality. Results: The novel HTK-N decreased ultrastructural alterations but otherwise presented limited effect on protecting small bowel from ischemia-reperfusion injury in vascular route. However, the additional luminal preservation led to positive impacts on the integrity of intestinal mucosa and vitality of goblet cells. In addition, vascular plus luminal preservation route with HTK significantly protected the intestinal tissue from edema. Conclusion: HTK-N protected the intestinal mucosal structure and graft vitality as a luminal preservation solution. Additional luminal preservation route in cold storage was shown to be promising.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 96%

A novel histidine–tryptophan–ketoglutarate formulation ameliorates intestinal injury in a cold storage and ex vivo warm oxygenated reperfusion model in rats

et al. 2020

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“…The individual preservation capacity of the five storage solutions was first analyzed for cocultures of HUVECs with macrophages and HepaRG with LX‐2 cells under static culture conditions. The storage solutions used were the vascular preservation solution TiProtec (termed low_Defer solution), a previously used derivative of it with a higher concentration of the iron chelator deferoxamine (termed standard), a chloride‐poor variant of the standard cell preservation solution in which the organic anion lactobionate was used as substitute for most of the chloride ions (termed low_Cl − solution), a variant of the standard cell preservation solution supplemented with dextran 40 000, a macromolecular additive which has been shown to enhance hypothermic lung storage, here designated dextran solution, and a variant in which PEG (average m.w. 35 000) was used as macromolecular alternative to dextran (termed PEG solution).…”

Section: Resultsmentioning

confidence: 99%

“…Cold storage of porcine lungs in Custodiol‐N could be improved by the addition of 50 g L −1 dextran 40 000 . This additive has also been used for the cold perfusion of porcine kidneys .…”

Section: Discussionmentioning

confidence: 99%

“…This additive has also been used for the cold perfusion of porcine kidneys . Functional studies in the cold‐stored lungs indicated less edema formation and better oxygenation after addition of dextran to Custodiol‐N . To test whether dextran also possesses beneficial effects on the storage of cocultures/simplified liver‐on‐chip models dextran 40 000 was added to dextran storage solution in the same concentration that has previously been used successfully as additive to the parent preservation solution Custodiol‐N, i.e., 50 g L −1 .…”

Section: Discussionmentioning

confidence: 99%

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Preservation of Cell Structure, Metabolism, and Biotransformation Activity of Liver‐On‐Chip Organ Models by Hypothermic Storage

Gröger

Dinger

Kiehntopf

et al. 2017

Adv Healthcare Materials

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The liver is a central organ in the metabolization of nutrition, endogenous and exogenous substances, and xenobiotic drugs. The emerging organ-on-chip technology has paved the way to model essential liver functions as well as certain aspects of liver disease in vitro in liver-on-chip models. However, a broader use of this technology in biomedical research is limited by a lack of protocols that enable the short-term preservation of preassembled liver-on-chip models for stocking or delivery to researchers outside the bioengineering community. For the first time, this study tested the ability of hypothermic storage of liver-on-chip models to preserve cell viability, tissue morphology, metabolism and biotransformation activity. In a systematic study with different preservation solutions, liver-on-chip function can be preserved for up to 2 d using a derivative of the tissue preservation solution TiProtec, containing high chloride ion concentrations and the iron chelators LK614 and deferoxamine, supplemented with polyethylene glycol (PEG). Hypothermic storage in this solution represents a promising method to preserve liver-on-chip function for at least 2 d and allows an easier access to liver-on-chip technology and its versatile and flexible use in biomedical research.

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NMR HRMAS spectroscopy of lung biopsy samples: Comparison study between human, pig, rat, and mouse metabolomics

Benahmed

Elbayed

Daubeuf

et al. 2013

Magnetic Resonance in Med

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A new preservation solution for lung transplantation: Evaluation in a porcine transplantation model

Cited by 27 publications

References 19 publications

A novel histidine–tryptophan–ketoglutarate formulation ameliorates intestinal injury in a cold storage and ex vivo warm oxygenated reperfusion model in rats

A novel histidine–tryptophan–ketoglutarate formulation ameliorates intestinal injury in a cold storage and ex vivo warm oxygenated reperfusion model in rats

Preservation of Cell Structure, Metabolism, and Biotransformation Activity of Liver‐On‐Chip Organ Models by Hypothermic Storage

NMR HRMAS spectroscopy of lung biopsy samples: Comparison study between human, pig, rat, and mouse metabolomics

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