A new protein-coupled antigen of α-conotoxin MI displays high immunogenicity and can produce antiserum with high detoxification activity

Zhang, Min; Yu, Shuo; Zhang, Xin; Huang, Qiuyuan; Huang, Yue; Luo, Min; Wei, Yuanmei; Chen, Wenwen; Chen, Ze; Zhou, Xiaowei; Dai, Qiuyun

doi:10.1016/j.toxicon.2022.01.009

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…At present, the methods for CTX-MI detection are extremely scarce; only high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) have been reported [ 13 , 36 ]. The former is more widely used in the purification of synthetic CTX-MI [ 12 , 36 ]. The latter is widely used to identify peptides and proteins, which was proved feasible for CTX-MI detection according to Yasushi’s report [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

“…CTX-MI is a short peptide of 14 amino acids (GRCCHPACGKNYSC-NH 2 ), which forms two disulfide bonds (Cys3-Cys8 and Cys1-Cys14) and selectively inhibits muscular acetylcholine receptors [ 11 ]. Due to a lack of clinical therapy and the unknown curative effect of antitoxic serums, the robust virulence of CTX-MI creates difficulties for prevention and clinical treatment [ 12 ]. In addition, the detection methods of CTX-MI rely on chromatography and mass spectrometry (MS) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI

Guo¹,

Deng²,

Zhao³

et al. 2022

Toxins

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Conotoxins (CTXs) are a variety of mixed polypeptide toxins, among which α-conotoxin MI (CTX-MI) is the most toxic. Serious toxic symptoms, a lack of counteracting drugs, and cumbersome detection processes have made CTX-MI a hidden danger for humans. One of the obstacles to resolving this problem is the absence of specific recognition elements. Aptamers have shown great advantages in the fields of molecule detection, drug development, etc. In this study, we screened and characterized aptamers for CTX-MI through a programmed process. MBMI-01c, the isolated aptamer, showed great affinity, with an affinity constant (KD) of 0.524 μM, and it formed an antiparallel G-quadruplet (GQ) structure for the specific recognition of CTX-MI. Additionally, an aptasensor based on the biolayer interferometry (BLI) platform was developed and displayed high precision, specificity, and repeatability with a limit of detection (LOD) of 0.26 μM. This aptasensor provides a potential tool for the rapid detection of CTX-MI in 10 min. The aptamer can be further developed for the enrichment, detoxification, and biological studies of CTX-MI. Additionally, the programmed process is applicable to screening and characterizing aptamers for other CTXs.

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