A new pseudo-peptide of Arg-Gly-Asp (RGD) inhibits intrahepatic metastasis of orthotopically implanted murine hepatocellular carcinoma

Basement Membrane Proteins Play an Important Role in the Invasive Processes of Human Pancreatic Cancer Cells

“…The RGD sequence is present in many ECM proteins and intracellular proteins, including FN, LN, osteopontin, and various collagens [23,24]. Synthetic RGD-containing peptides may bind several integrins, especially ␣ 3 ␤ 1 and ␣ 5 ␤ 1 , and they competitively occupy integrin receptor-binding sites, thus suppressing communication between tumor cells and BM proteins, with a resulting effect on adhesion, migration, and tumor metastasis [25][26][27]. Despite these observations, the definite roles of BM proteins in the invasive process of malignant tumors are still not clear, and studies on the interaction of BM proteins and integrins have been rare.…”

In vitro assessment of the cytotoxic effects of novel RGD analogues

“…The peptide purity was monitored on a RP-HPLC column Atlantis TM dC 18 , 4.6×150 mm, particle size 5μm, mobile phase: acetonitrile/deionised water 40/60 (v/v), at 25°C, flow rate: 1ml/min, and UV detection -206 nm. The correct molecular masses were confirmed by ESI-MS. For R(NO 2 )GD, GF -C 12 …”

“…Examples of such radio-imaging agents include [99mTc]apticide, which can be used in imaging deep vein thrombosis [10]. Several modifications of RGD peptides, including polymerisation, coupling with carriers and substitution by peptidomimetics, were performed to enhance the anti-tumour properties and lengthen the degradation time in vivo [11][12][13][14]. RGD peptides conjugated with different cytostatic agents are likely to exhibit an antitumour and antiangiogenic synergetic effect.…”

Basement membrane proteins play an active role in the invasive process of human hepatocellular carcinoma cells with high metastasis potential

“…We also found that GRGDS, in vitro, can bind to b1 integrins and switch on the downstream signal transduction, consequently enhancing the mobility and MMP secretion of HCC cells, so the effects of GRGDS on invasion and metastasis are ambivalent. Recent reports showed that some chemical modification of the original RGD peptide can achieve the property of inhibiting the degradation of the extracellular matrix by MMP-2 and MMP-9 in vitro (Fujii et al 1998;Tsuchiya et al 2002).…”

“…The RGD sequence is present in many extracellular matrix proteins and intracellular proteins, including FN, LN, osteopontin, and various collagens (Cachau et al 1989). Synthetic RGD-containing peptides may bind several integrins, especially a3b1 (VLA-3) and a5b1 (VLA-5), and competitively occupy the binding sites of the integrin receptors, thus suppress the communication of tumor cells with BM proteins, and affect adhesion, migration, and tumor metastasis (Zitzmann et al 2002;Tsuchiya et al 2002;Okroj et al 2003).…”