A new quantitative method for pseudouridine and uridine in human serum and its clinical application in acute myeloid leukemia

He, Ruijia; Qiao, Jing; Wang, Xiaoxia; Chen, Wenlian; Yin, Tong

doi:10.1016/j.jpba.2022.114934

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…The purpose of nucleoside extraction is to improve separation and detection efficiency and obtain higher signals of the analytes by removing salts, proteins, and uninterested nucleosides, especially large amounts of normal nucleosides, from the matrix. The initial extraction methods enrich free nucleosides from biological samples such as serum and urine, normally based on hydrophobic or hydrophilic interactions, ion exchange, and affinity with 1,2-cis-diol compounds with commercial materials, such as HLB [ 64 , 65 ] and WCX [ 66 ], as well as new materials such as graphene [ 67 ], polymers [ 68 ], metal oxides [ 69 , 70 , 71 ], and boronate-decorated substrates [ 72 , 73 , 74 ], which are also used for the extraction of nucleoside products after enzymatic digestion to further increase signal response. The shapes of substrate and extraction devices influence extraction efficiency [ 75 , 76 ].…”

Section: Qualitative and Quantitative Analysismentioning

confidence: 99%

Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery

Liu,

Dong,

Shen

et al. 2024

IJMS

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Nucleic acid modifications play important roles in biological activities and disease occurrences, and have been considered as cancer biomarkers. Due to the relatively low amount of nucleic acid modifications in biological samples, it is necessary to develop sensitive and reliable qualitative and quantitative methods to reveal the content of any modifications. In this review, the key processes affecting the qualitative and quantitative analyses are discussed, such as sample digestion, nucleoside extraction, chemical labeling, chromatographic separation, mass spectrometry detection, and data processing. The improvement of the detection sensitivity and specificity of analytical methods based on mass spectrometry makes it possible to study low-abundance modifications and their biological functions. Some typical nucleic acid modifications and their potential as biomarkers are displayed, and efforts to improve diagnostic accuracy are discussed. Future perspectives are raised for this research field.

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Section: Qualitative and Quantitative Analysismentioning

confidence: 99%

Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery

Liu,

Dong,

Shen

et al. 2024

IJMS

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Supercritical Fluid Chromatography in Bioanalysis–A Review

Toribio,

Martín,

Bernal

2024

J of Separation Science

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In the last decade, the instrumentation improvements in supercritical fluid chromatography (SFC) and the hyphenation to mass spectrometry (MS), have increased the SFC acceptance between scientists, becoming today a valuable tool in analytical chemistry. The unique selectivity, short analysis times, low consumption of organic solvents, and the greener mobile phase, have contributed to expanding its applicability which has led to an increase in the number of publications especially in the bioanalysis area. This work reviews the advantages and main applications of SFC in bioanalysis during the last 5 years. Fundamental aspects concerning mobile phase composition, stationary phase, hyphenation to MS as well as matrix effect have been discussed. Finally, the most relevant applications have been summarized.

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From Modification Maps to Clinical Impact: RNA Epigenetics in Cancer Diagnosis and Treatment

Sweef,

Asfar,

Choi

et al. 2024

Interdisciplinary Cancer Research

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A new quantitative method for pseudouridine and uridine in human serum and its clinical application in acute myeloid leukemia

Cited by 3 publications

References 31 publications

Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery

Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery

Supercritical Fluid Chromatography in Bioanalysis–A Review

From Modification Maps to Clinical Impact: RNA Epigenetics in Cancer Diagnosis and Treatment

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