A New Rat Colon Cancer Cell Line Metastasizes Spontaneously: Biologic Characteristics and Chemotherapeutic Response

Abstract

A new cell line (RCN‐9) was established in culture from a transplantable rat colon adenocarcinoma, which was induced in the colon of a male Fischer F344 rat by subcutaneous administration of 1,2‐dimethylhydrazine. When RCN‐9 cells were injected subcutaneously or into the cecal subserosa of syngeneic rats, carcinomas with progressive growth were obtained and the development of lung (63.6%) and liver (40.0%) metastases, respectively, ensued. Antitumor effects of 5‐fluorouracil (5‐FU), adriamycin (ADM) and mitomycin C (MMC) against RCN‐9 were examined in vivo and in vitro. 5‐FU and ADM had antitumor effects both in vivo and in vitro; MMC had antitumor effects in vitro. These results show that the RCN‐9 cell line can be used both as a model to study mechanisms of metastasis from colon carcinoma and as a model in chemotherapeutic studies of metastatic disease from colon carcinoma.

Kupffer cells of cirrhotic rat livers sensitize colon cancer cells to Fas-mediated apoptosis

“…The colon adenocarcinoma cell line RCN-9, a dimethylhydrazineinduced colon carcinoma of Fischer rats (Inoue et al, 1991), was obtained from ATCC (Rockville, MD). RCN-9 cells were maintained in DMEM with 10% FCS and 0.2 mmol l -1 l L-glutamine (Sigma Chemical Co, St Louis, MO, USA).…”

Polysaccharide-K (PSK) may suppress surgical stress-induced metastasis in rat colon cancer

“…This cell line, a subclone of RCN-9 established by Inoue et al [11], has high potential for liver metastasis. RCN-H4 cells were cultured in RPMI 1640 medium (Sigma, St. Louis, MO, USA) supplemented with 10% fetal calf serum (Gibco, Grand Island, NY, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin, in an incubator at 37°C with an atmosphere of 95% air and 5% CO 2 .…”

Leukocyte Behavior in Angiogenic Vessels Is Affected by Tumor-Derived Nitric Oxide

“…The line of RCN-9 tumor cells (Riken Cell Bank, Tsukuba, Japan) established from Fischer 344 rat colon carcinoma [21] was intraperitoneally inoculated according to the method described by the authors [22]. Briefly, 1ϫ10 7 cells were inoculated into the peritoneal cavity of 7-week-old male Fischer 344 rats (136-198 g; Charles River, Kanagawa, Japan).…”